Preparation of diagnostic lysate antigens for rapid evaluation of local entero-3 vaccine

Document Type : Original Article


1 Veterinary Serum and Vaccine Researches Institute, Abbassia, Cairo, Egypt.

2 Central Laboratory for Quality control of Veterinary Biologics, Abbassia, Cairo, Egypt


Studies were conducted to determine the utility of lysate antigens for rapid
evaluation of the local entero-3 vaccine, antigens were prepared from cell cultures
infected with bovine rota virus (BRV) and bovine corona virus (BCV) as well as
from Enterotoxigenic E. coli strain K99. Prepared antigens were tested with field
serum samples collected from both late pregnant entero-3 vaccinated cows and their offsprings using different serological assays including: microagglutination test,
indirect ELISA and immunofluorescent antibody technique. Results of this endeavor
were correlated to that of the standard virus neutralization test. The locally prepared
antigens were proved useful for vaccine evaluation. Moreover, these antigens are
recommended for both detection and assessment post vaccination or post infection of sero-conversion against BRV, BCV and E. coli.


Main Subjects