Two hundred, five-week old chickens were divided into six equal groups (30 chicks/group) and vaccinated with different live NDV vaccines in Nigella sativa oil. The vaccinated birds were bled at one-week interval post-vaccination over six weeks and the collected sera were tested by the HI test. Half of each group was challenged 21-days post-vaccination by intramuscular route of inoculation with 2X105EID50 of the velogenic viscerotropic Newcastle disease virus (VVNDV). Birds were observed for 15 days post-challenge for any clinical signs of Newcastle disease. Deaths within this period were subjected to post mortem examination. The remaining birds in each group were observed after challenge till 42-days post-vaccination.
Hussien, A. S., & Madbouly, H. M. (2005). Vaccination of chickens with live Newcastle disease virus vaccines adjuvanated with Nigella sativa oil. Journal of Veterinary Medical Research, 15(2), 123-126. doi: 10.21608/jvmr.2005.77942
MLA
A. S. Hussien; H. M. Madbouly. "Vaccination of chickens with live Newcastle disease virus vaccines adjuvanated with Nigella sativa oil". Journal of Veterinary Medical Research, 15, 2, 2005, 123-126. doi: 10.21608/jvmr.2005.77942
HARVARD
Hussien, A. S., Madbouly, H. M. (2005). 'Vaccination of chickens with live Newcastle disease virus vaccines adjuvanated with Nigella sativa oil', Journal of Veterinary Medical Research, 15(2), pp. 123-126. doi: 10.21608/jvmr.2005.77942
VANCOUVER
Hussien, A. S., Madbouly, H. M. Vaccination of chickens with live Newcastle disease virus vaccines adjuvanated with Nigella sativa oil. Journal of Veterinary Medical Research, 2005; 15(2): 123-126. doi: 10.21608/jvmr.2005.77942
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