Bovine Neurobrucellosis: Pathological and Bacteriological Studies

Document Type : Original Article

Authors

1 Department of Pathology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt

2 Department of Microbiology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt

Abstract

Thirty two cattle, had no neurological syndrome, were serologically positive to brucellosis by using Tube agglutination and Rose Bengal Tests, by the official veterinary authority in Beni Suef province, Egypt. These animals were slaughtered in Beni Suef abattoirs during the project of control and eradication of brucella positive animals in Beni Suef province. Postmortem examination was performed and the brain was mechanically removed. Longitudinally the brain was cut; one half was fixed in formalin 25% for 2 weeks and the other one were sent to microbiology department for bacteriological isolation. Transverse sections were done in the fixed
tissue and samples were collected from cerebrum, cerebellum, medulla oblongata, thalamus, hypothalamus, and caudate nucleus. These samples were processed according to Bancroft and Gamble (2008). From the thirty two brain samples, no isolates of brucella species were recovered. From the thirty two brain samples, no isolates of brucella species were recovered. Brains of slaughtered animals showed no pathological lesions grossly. Microscopically, inflammatory reactions, degeneration, malacia, demyelination, pigmentation,and vascular changes were detected.

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