Prevalence of Salmonellosis in Broilers and Native Breed Chicks in Beni Suef Governorate, Egypt

Fahmy, Samia Sayed; Shany, Salama; Dahshan, Al-Hussein; Nasef, Soad; El Sawah, Azza

doi:10.21608/jvmr.2023.221528.1088

Prevalence of Salmonellosis in Broilers and Native Breed Chicks in Beni Suef Governorate, Egypt

Document Type : Original Article

Authors

¹ Department of Poultry Diseases, Faculty of Veterinary Medicine, Beni-Suef University, Beni Suef 65211, Egypt.

² Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute.

10.21608/jvmr.2023.221528.1088

Abstract

Salmonellosis is an economic burden on poultry producers’ shoulders and also, it is a very critical problem to human health. Resistance of non-typhoidal Salmonellae to antimicrobials is a serious problem. In the current study, prevalence of avian salmonellosis in both commercial broiler and native breed farms in Beni Suef from March, 2018 through October, 2019 were investigated. Samples were collected from 31 chicken farms including broilers (N=13) and native breed (N=18). Specimens were collected from liver and caecum (5 chicks per flock) of clinically suspect cases aging 1-15 days old. Bacteriological examination revealed that 6 isolates (19.35% isolation rate) were successfully cultivated including 2/13 (15.38%) and 4/18 (22.22%) isolates for broilers and native breed farms, respectively. Serotyping of different isolates revealed the following serovars; S. kentucky 8,20:i:z 6 in 4 farms (1 broiler and 3 native breed), S. bonariensis 6,8:i:e,n,x in one broiler farm and S. rechovot 8,20:e,h:z6 in one native breed flock. Antimicrobial sensitivity testing of different Salmonella serovars showed 100% sensitivity to fosfomycin, 66.6% to amoxicillin/clavulinic acid, 66.6% to florfenicol, 66.6% to polymyxin- B, 33.3% to gentamycin and 16.66% sensitivity to each of kanamycin, neomycin, apramycin and enrofloxacin. S. bonarensis was found resistant to amoxicillin/clavulinic acid and of intermediate sensitivity to ciprofloxacin. Molecular screening of different serovars to virulence and drug resistance genes indicated 100% positivity to invA, stn, avrA virulence genes and also the tested antimicrobial genes except qnrA that was detected in only one of the tested Salmonellae.

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