The role of corynebacterial phospholipase D vaccine in activation of macrophages

Document Type : Original Article

Authors

1 Biotechnology Center for Services and Researches, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt.

2 Department of Microbiology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt.

Abstract

The role of phospholipase D toxoid (PLD) vaccine in enhancing killing activity of macrophages was demonstrated in this study. Four groups of Balb/c mice were vaccinated with different forms of current vaccines against Corynebacterium pseudotuberculosis (C. pseudotuberculosis). The first group was vaccinated with purified recombinant mutated PLD protein adjuvated vaccine; the second with formalin inactivated whole cells of C. pseudotuberculosis adjuvated vaccine, the third group with combined bacterin-toxoid adjuvated vaccine and the fourth was given viable C. pseudotuberculosis cells. Mononuclear peritoneal cells from each vaccinated groups were collected and inoculated intraperitoneally into naïve recipient Balb/c mice that were subsequently challenged by equal number of live C. pseudotuberculosis cells. Killing activity of peritoneal macrophages collected from each recipient group of mice was assayed by cultivation of lysed macrophages on plates of count brain heart agar. It was reported that the highest killing activity of macrophages were those collected from mice vaccinated with recombinant PLD adjuvated vaccine that reaches 95% of phagocytosed C. pseudotuberculosis living bacteria; where those given viable C. pseudotuberculosis bacteria (80%); then combined vaccine (69.5%) and the least killing activity was performed by macrophages obtained from bacterin vaccinated animals

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