Molecular characterization of Corynebacterium pseudotuberculosis isolated from sheep by random amplified polymorphic DNApolymerase chain reaction (RAPD-PCR)

Goda, Azza S.A.; Mahmoud, Mona A.; Osman, Wafaa A.; Ziada, N.A.; Moussa, I. M.

doi:10.21608/jvmr.2007.77892

Molecular characterization of Corynebacterium pseudotuberculosis isolated from sheep by random amplified polymorphic DNApolymerase chain reaction (RAPD-PCR)

Document Type : Original Article

Authors

¹ Animal Health Department, Desert Research Center, Materia,Cairo, Egypt.

² Animal Health Department, Desert Research Center, Materia,Cairo, Egypt

³ Department. of Microbiology, Faculty of Veterinary Medicine, Cairo University, Gizza, Egypt

10.21608/jvmr.2007.77892

Abstract

The phenogenetic and phylogenetic relationships among 7 identified strains of C.
pseudotuberculosis isolated from sheep were checked by PCR specific primers demonstrated with random amplified polymorphic DNA – polymerase chain reaction (RAPD – PCR) by using five arbitrarily primers. C pseudotuberculosis isolates were distinguished according to the banding patterns of their amplified DNA on agarose gel. The variation can be used for diagnostic differentiation among C. pseudotuberculosis strains. Differences were observed in RAPD patterns between the 7 isolates and this may be due to the presence of novel serovars of this microorganism.

Keywords