Preparation of a trivalent oil-emulsion vaccine of inactivated Newcastle disease virus, infectious bronchitis virus and Haemophilus paragallinarum

Document Type : Original Article

Authors

1 Veterinary Serum and Vaccine Research Institute , Abbasia. Cairo, Egypt

2 Veterinary Serum and Vaccine Research Institute , Abbasia. Cairo, Egypt.

Abstract

Monvalent inactivated oil-emulsion, Newcastle disease virus (NDV), infectious bronchitis virus (IBV) and Haemophilus paragallinarum vaccines as well as a trivalent one (NDV + IBV + HP) were prepared. The cellular and humoral immune responses of chicken to these vaccines were evaluated by using lymphocytes blastogenesis assay, haemagglutination-inhibition, serum neutralization, tube agglutination and challenge tests. The results showed that the trivalent vaccine was safe, efficient as monovalent vaccines and protected chicken against Newcastle, Infectious bronchitis and infectious coryza

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