Staphylococcus aureus protein "A" as a rapid and sensitive test in comparative with other serological tests for detection of Rift Valley Fever antibodies in vaccinated camels

Document Type : Original Article

Authors

1 Animal Reproduction Research Institute, Al-Haram, Giza

2 Animal Health Research Institute, Dokki, Giza.

3 Veterinary Serum and Vaccine Research Institute, Abbassia, Cairo.

Abstract

Rift Valley Fever (RVF) is a viral zoonotic disease transmitted by mosquitoes leading to abortion and death mainly in livestock. The present study evolved serum samples obtained from 100 RFV vaccinated as well as 10 non vaccinated camels tested for RVF antibodies using different serological techniques. Freshly prepared Staphylococcus aureus protein "A" (SPA) was used as a rapid, sensitive, specific and cheap screening test which did not need specific or expensive equipment. Also uses of SPA-ELISA test without need antispecies conjugate, SNT and AGPT. Inactivated RVF virus was used in all serological tests except SNT. The results showed that Staphylococcus aureus Protein Acoagglutination test (SPA-COAT) was 78%, SPA-ELISA 82%, SNT 75% and AGPT 30%. The degree of positive samples with (++++) and (+++) of SPA-COAT were also positive in SPA-ELISA and SNT, but samples with (++++) SPA-COAT were only positive in AGPT. We could recommend the use of SPA-COAT, as a universal technique, in wide range of veterinary medicine as an effective and economic method which acts a non species specific for serological screening field test as well as a laboratory test

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