Comparative studies on anti-avian sera conjugated with fluorescin isothiocyanate

Khodeir, M. H.; M. El-Sadek, Ghada; A. El-Ibiary, Elham

doi:10.21608/jvmr.2020.77621

Comparative studies on anti-avian sera conjugated with fluorescin isothiocyanate

Document Type : Original Article

Authors

¹ Veterinary Serum and Vaccine Research Institute, Cairo

² Central Laboratory for Quality Control of Veterinary Biologics, Cairo, Egypt.

³ Central Laboratory for Quality Control of Veterinary Biologics, Cairo, Egyp

10.21608/jvmr.2020.77621

Abstract

Rabbit antisera were successfully prepared against chickens; turkey; ducks; geese; pigeons and quails as antispecies and conjugated with fluorescein isothiocyanate (FITC). The ability to use these antisera as homologous and heterologous antispecies was studied through the application of indirect fluorescent antibody technique (FAT) using specific antigens and antibodies of Newcastle; infectious bursal disease virus; duck plague virus; duck hepatitis virus; fowl cholera and pigeon paramyxo virus. The results showed that Titration of the prepared anti avian sera conjugated with FITC induced strong positive FAT reactions up to dilutions of 1:10000; 1:1000; 1: 100000; 1: 1000; 1: 100000 and 1:1000 for anti- chicken; anti-Turkey; ant-duck; ant-goose; anti-pigeon and antiquill sera respectively. It was found that homolgous species anti-sera showed strong positive FAT reaction (green apple fluorescing) scored as 4+ while hetrologous species anti-sera showed less degree of positive reactions ranged from 3+ to ± reaction showing that the usefulness of anti-sera as reagents in serological techniques is limited by the homogeneity and heterogeneity of such antisera. So, the present preparations could be used to low extent as heterologous anti-avian sera.

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