Phenotypic and genetic characterization of fowl pox and turkey pox viruses

E. Nakhla, Olfat; Soliman, Y. A.; Taha, M. M.

doi:10.21608/jvmr.2020.77620

Phenotypic and genetic characterization of fowl pox and turkey pox viruses

Document Type : Original Article

Authors

¹ Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo

² Central Laboratory for Evaluation of Veterinary Biologics, Abbasia, Cairo, Egypt

³ Central Laboratory for Evaluation of Veterinary Biologics, Abbasia, Cairo, Egypt.

10.21608/jvmr.2020.77620

Abstract

Fowl and turkey pox viruses were analyzed for their heterogeneity on the basis of protein profile, western blotting, PCR analysis and restriction endonuclease analysis. On the protein level, only a single band with a WM of 115KDa was seen with turkey pox virus but not with the fowl strain, and a band reacted at 10KDa with Turkey but not fowl pox virus in western blotting analysis other protein profiles were nearly the similar. PCR amplification of the lateral terminal repeat (LTR) region of both viruses confirmed a single band migrating about 900 bp with both strains and restriction digestion proved the homology between both strains.

Main Subjects