Comparative Efficacy of Manuka Honey and its Combination with Fusidic Acid in Surgically Inflicted Methicillin Resistance Staphylococcus aureus Infected Wounds in Rabbits

Document Type : Original Article

Authors

1 Pioneer Pets Hospital, Rawalpindi, Pakistan.

2 National Veterinary Laboratory, Pakistan.

3 Clinical Medicine & Surgery, University of Agriculture, Faisalabad, Pakistan.

Abstract

Wound healing is a complex mechanism that involves recruitment and pragmatic proliferation of cells at the site of injury and it may be delayed due to many reasons including wound infection. Topical use of antimicrobials/antibiotics is one of rationale for wound care. However, development of resistance to antimicrobials is an emerging and serious problem for the clinicians over the globe. The present study was, therefore, been planned to evaluate the comparative efficacy of Manuka honey (MH) alone and in combination with fusidic acid (FA) in the treatment of surgically inflicted full thickness MRSA infected wounds in rabbits. Adult male rabbits (n=30) were randomly divided into 3 groups (A to C) of equal size. A set of full-thickness skin wound (3 on each rabbit) were inflicted on the dorsolateral aspects of lumber region of each rabbit. Then infection was induced with 10 µl of 0.5 McFarland turbidity standard suspension of MRSA and waited for 24 hours to let the infection develop. Wounds in groups A and B were respectively treated with MH and FA, whereas, group C received a combined therapy comprising a mixture of MH and FA in equal proportions. The wound healing was evaluated in terms of rate of wound contraction, healing time and histopathological evaluations. The data was then subjected to statistical analyses using analysis of variance (ANOVA) considering it significant at p<0.05 and highly significant at p<0.01.

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Journal of Veterinary Medical Research

Articles in Press, Accepted Manuscript
Available Online from 24 August 2024

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