In-vitro Evaluation of Different Commercial Antimycotics and Disinfectants against Trichophyton verrucosum Isolated from Beef Farm in Beni Suef, Egypt

Document Type : Original Article


1 Department of Hygiene, Zoonoses and Epidemiology, Faculty of Veterinary Medicine, Beni-Suef University

2 Veterinary medicine department, Faculty of veterinary medicine, Beni-suef university


Dermatophytosis is a fungal disease that affects both animals and humans. The improper use of antimycotics and disinfectants led to an acquired resistance of dermatophytes to most of the commonly used antimycotic agents. The aim of the present study was to estimate the prevalence of dermatophytosis in a calf-beef farm and to evaluate the in-vitro efficacy of six antifungal agents and three disinfectants. Out of the 120 examined calves, 42 (35%) showed ringworm lesions. Trichophyton verrucosum was isolated from a total of 45 samples including 42 skin scrapping from infected animals and 3 hair samples from farm workers followed by molecular identification using PCR. Antimicrobial sensitivity profile was performed by agar-based disk diffusion method using six antifungal agents. Three disinfectants with different concentrations were tested against 45 strains of T. verrucosum isolates using broth macro-dilution at different contact times (20 sec, 30min, 1h and 24h). Animal isolates were sensitive to Fluconazole (100%), meanwhile human strains were sensitive to Itraconazole (66.7%) (P<0.001). Recovered isolates were sensitive to iodine (7%) at contact time 1h and 24h (47 and 69 %, respectively) at P<0.001, and to Virkon S (1%) after 24 h contact time (55%) at P<0.001. Results prove the antimycotic action of Fluconazole and Itraconazole on Trichophyton verrucosum and highlights the significant role of increasing the contact time on decreasing the resistance pattern of dermatophytes to Virkon S (1%) and Iodine (7%) that give a promising results when used to control the infections in the surrounding environment.


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