Identification and Characterization of Salmonella Species Isolated from Broiler Chickens

Document Type : Original Article


1 Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt.

2 Chef Researcher of Microbiology, Reference Lab. of Quality Control on Poultry Production-Animal Health Research Institute (AHRI).

3 Department of Poultry Diseases, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt.

4 Department of Bacteriology, Animal Health Research Institute, Beni-Suef Laboratory, Agricultural Research Center, Egypt.


Salmonellosis is one of the most important problems in poultry industry and a critical food safety hazard. In the present study the prevalence of avian Salmonellosis was studied in different farms of broiler chickens in Beni Suef Governorate, Egypt during the period from January to April 2020. A total of 140 samples were taken from slaughtered diseased or freshly dead broiler chickens aged from one to 35 days. Bacteriological examination revealed that 7.14% of the samples were Salmonella positive. Serotyping of Salmonella isolates showed that S. kentucky, S. blegdam and S. virchow were recognized at rates of 40%, 30% and 30%, respectively. Antimicrobial susceptibility test revealed that all salmonella isolates were multidrug resistant (MDR). All isolates were resistant to oxytetracycline (100%) while 90% were resistant to amoxicillin-clavulanic acid, cefotaxime, sulfamethoxazole- trimethoprim and norofloxacin.  On the other hand, 80% of isolates were sensitive to fosfomycin and nitrofurantoin. Results of screening of some MDR isolates by multiplex PCR for detection of some virulence genes showed that all the tested isolates (100%) had invA, stn, spvC genes meanwhile pefA was not detected in any isolate.


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