Beni-Suef University; Faculty of Veterinary MedicineJournal of Veterinary Medical Research2357-051222120130301Prevalence and Antibiogram of E. coli in Broiler Chickens Reared in Libya127766610.21608/jvmr.2013.77666ENAAFaculty of Veterinary Medicine, Omar Almukhtar University, LibyaJournal Article20200317Eighty five E.coli isolates were isolated from 3-6 weeks old broiler chickens suspected to be suffering from colibacillosis, complicated CRD, diarrhea, arthritis, slow growth rate and high mortality up to 15% from the examined 300 broiler chickens from different flocks. 250 (83.33%) were infected with E.Coli and all flocks were (100%) positive for colibacillosis where 85 E.coli isolates were obtained from which, only 42 isolate were sero-grouped into O55 (5.88%), O26 (8.23%), O111 (3.52%), O128 (14.11%), O125 (2.35%), O86 (8.23%), O126 (7.05%) while 43 isolates cannot be sero-grouped according to the available antisera. The invitro sensitivity of the isolated strains to various chemotherapeutic agents revealed that all strains were sensitive to Kanamycin and Amoxacillin (100%). All strains were resistant to Trimethoprim. In vitro pathogenicity testing of the isolated E.coli using Congo red medium revealed that all isolated strains were pathogenic (Congo red positive)Beni-Suef University; Faculty of Veterinary MedicineJournal of Veterinary Medical Research2357-051222120130301The Correlation between M. bovis Isolation and ELISA Using PPD-B and ESAT6-CFP10 Mixture on the Sera of Tuberculin Reactor Cattle and Buffaloes457766810.21608/jvmr.2013.77668ENI.A.RadwanFaculty of Veterinary Medicine, Beni- Suef University, Beni-Suef 62511, EgyptW.H.HassanFaculty of Veterinary Medicine, Beni- Suef University, Beni-Suef 62511, EgyptE.ANasrFaculty of Veterinary Medicine, Beni- Suef University, Beni-Suef 62511, EgyptA. H.AbedFaculty of Veterinary Medicine, Beni- Suef University, Beni-Suef 62511, EgyptJournal Article20200317In this study, 6150 animals (3600 dairy cattle and 2550 buffaloes) in different Egyptian governorates were tested by single intradermal cervical tuberculin test using PPD-B, 72 cattle (2%) and 26 buffaloes (1%) reacted positively. After slaughtering the PM examination revealed 49 (68.1%) of the slaughtered cattle showed visible lesions (VL); comparing with 17 (65.4%) in buffaloes, while 23 (31.9%) showed non visible lesions (NVL) comparing with 9 (64.6%) in buffaloes. The bacteriological examination of processed samples from the 72 slaughtered cattle<br />revealed 44 M. bovis isolates (61.1%); 40 (81.6%) were from 49 VL and 4 (17.4%) were from 23 NVL, compared to 15 (57.7%) Mycobacterial isolates from buffaloes; 10 (38.5%) were M .bovis, all were from the 17 VL (58.8%), and 5 isolates (19.2%) were MOTT, 3 (17.6%) were from the 17 VL and 2 (22.2%) were from the 9 NVL. The ESAT6-CFP10 mixture using ELISA of the tuberculin positive animals sera showed VL could detect 83.7% and 70.6% in cattle and buffaloes, respectively; compared to 89.8% and 76.5% in cattle and buffaloes, respectively using PPD-B. On the other hand, the sera collected from tuberculin positive animals with NVL, the antigen mixture revealed 17.4% and 22.2%in cattle and buffaloes, respectively; compared to 21.7% in cattle and 33.3% in buffaloes with the PPD-B. The PCR assay using Oligonucleotide primer that amplifies a 350bp fragment in RD7 region of M. bovis confirmed the cultural and biochemical identification of M. bovis isolates (PCR-positive) and MOTT (PCR-negativeBeni-Suef University; Faculty of Veterinary MedicineJournal of Veterinary Medical Research2357-051222120130301Two Dimensional and M-Mode Echocardiography in Normal Healthy Egyptian Zarabi Goats567766910.21608/jvmr.2013.77669ENT.MiskFaculty of Veterinary Medicine, Sadat City- Menoufia University, EgyptM.NayelFaculty of Veterinary Medicine, Sadat City- Menoufia University, EgyptA.El-SifyFaculty of Veterinary Medicine, Sadat City- Menoufia University, EgyptJournal Article20200317Echocardiography and intra-cardiac dimensions have not previously been reported in adult egyptian zarabi goats despite its potential application for medical purpose. The chests of 11 adult male goats were examined at 3th - 6th intercostal space on both sides of the thorax using 2.6-6 MHz convex transducer. All chambers of the heart were clearly visualized in the parasternal long axis view from both sides. The both ventricles and ventricular outflow with appearance of the aortic root is best to visualize from the right side at 4th RICS (right inter costal space) and the transducer rotate bet 0°to30°caudally. Left ventricle with papillary muscle, chordea tendenea, mitral valve, aortic valve and pulmonary artery views were visualized at 4th RICS (right inter costals space) 2 cm above elbow and the transducer rotate at different angles bet 0°to90° perpendicularly on inter costals space. During systole and diastole, dimensions of the left ventricle were greater than those of right ventricle, whereas size of the ventricles was significantly greater during diastole than<br />systole (p>0.01). The dimensions of the interventricular septum, left and right ventricular free wall were greater in systole than diastole.Beni-Suef University; Faculty of Veterinary MedicineJournal of Veterinary Medical Research2357-051222120130301Bovine Mammary Adenocarcinoma: A case report347767010.21608/jvmr.2013.77670ENE. A.MahdyFaculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, EgyptS.DeebFaculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, EgyptJournal Article20200317In a collection of udder of cattle obtained from slaughter house to study mastitis a case was diagnosed as intraductal carcinoma. At necropsy, the condition was not recognized grossly.Microscopically,the udder tissue showed tubular structures occupied a wide areas. The tumors was characterized by anplastic cell proliferation forming ductal and aciner structures, some of which had papillary projection. Signs of malignancy were prominent including pleomorphism, increased mitotic activity and hyperchromatism. On the basis of the histopathology, this type of tumor was diagnosed as adenocarcinoma. This case can be classified as "grade II".Beni-Suef University; Faculty of Veterinary MedicineJournal of Veterinary Medical Research2357-051222120130301Protective Effect of Dates (Phoenixdactylifera L.) and Licorice (Glycyrrhizaglabra) on Carbon Tetrachloride–Induced Hepatotoxicity in Dogs897767210.21608/jvmr.2013.77672ENS. IFathallahFaculty of Veterinary Medicine, Menofia University (Sadat), Menofia, EgyptA. A.MohamedFaculty of Veterinary Medicine, Menofia University (Sadat), Menofia, EgyptH. K.JunAl- Farabi Dental and Nursing College- Jeddah-K.S.A.D. H.KimDepartment of Veterinary Medicine, College of VeterinaryMedicine, ChungnamNationalUniversity, Daejeon, KoreaJournal Article20200317The ameliorative activity of aqueous extracts of the dates (Phoenix dactyliferaL.) and Licorice (Glycyrrhizaglabra) on carbon tetrachloride (CCl4)-induced hepatotoxicity was studied in dogs. Fifteen dogs were divided into 3 equal groups of five each. Group 1 (CCl4-positive control) animals were injected intra-peritoneal with a fresh mixture of equal volumes of CCl4 and olive oil (1:1 – v: v) at doses of 0.6 ml/kg of body weight for 3 successive days and receiving normal saline orally (15ml/kg) for 14 consecutive days. Group 2 (prophylactic group) receiving both date and licorice<br />extracts orally for 24 consecutive days and treated with IP CCl4 on Days 10, 11, and 12 of the experimental period. Group3 (curative group) were received aqueous extract of date and licorice extracts for 14 consecutive days and were injected with IP CCl4 on Days 1, 2, and 3 of the experimental period. Liver damage was assessed by liver morphology, histology and estimation of plasma concentration of enzyme activities of alkalinphospatase (ALP), gamma-glutamyltransferase (GGT), aspartate aminotransferases (AST) and alanine aminotransferase (ALT), and protein profile (total proteins, albumin, globulin and A/G ratio). Treatment with aqueous extract of date flesh and licorice significantly reduced CCl4-induced elevation in plasma liver marker enzymes concentrations and ameliorated histopathological liver damage and stop fibrosis and edema of hepatic parenchyma in dogs. Hence these synergistic effects of date and licorice extract can be used alone and/or in polyherbal formulations of hepatoprotective drugs, there by preventing the process of initiation and progress of hepatocellular diseasesBeni-Suef University; Faculty of Veterinary MedicineJournal of Veterinary Medical Research2357-051222120130301Overview on Veterinary Acupuncture127767410.21608/jvmr.2013.77674ENA. A.MohamedFaculty of Veterinary Medicine, Menofia University (Sadat Branch), EgyptJournal Article20200317Acupuncture has been used in veterinary medicine by the Chinese for thousands of years. It has been performed on many species of animals but it is most commonly done on dogs, cats, horses, cattle and birds. Acupuncture is a method of producing analgesia or altering the function of a system of the body by inserting fine, flexible, thin needles into the skin at specific sites on the body (acupoints) along a series of<br />invisible lines or channels called meridians to balance the body’s vital energy, or “Qi or Chi“. There are 14 different meridians and through these meridians the Qi flows. The needles may be energized electrically, or warmed. According the Traditional Chinese Medicine theory, when the Qi can not properly flow from one acupuncture point to the next, a disease state occurs. Therefore, the goal of acupuncture therapy is to unblock the stagnation and allow the Qi to flow properly throughout the body. With a free flow of Qi, the body is in balance, and there is no disease. The exact mechanism of acupuncture is under active investigation. Until recently, it was thought that each needle acts as an external stimulus which encourages the release of chemicals, natural painkillers called endorphins that are believed to initiate self-healing within the body. These changes then help to improve circulation, relieve muscle spasms, stimulate nerves, relieve pain and stimulate the body’s immune system. However, magnetic resonance imaging researches reported that comparison of images of brains of subjects with and without acupuncture indicated that the acupuncture deactivates areas of the brain that control pain. The use of acupuncture in conjunction with drugs and/or surgery can greatly improve your animal’s chances for a rapid and complete recoveryBeni-Suef University; Faculty of Veterinary MedicineJournal of Veterinary Medical Research2357-051222120130301Isolation and Identification of EHV-I from Aborted Mare at Early Stage Pregnancy677767510.21608/jvmr.2013.77675ENNashwaK. MadkourVeterinary Serum and Vaccine Researsh Institution, Abbasia, Cairo, EgyptNehalSalehDepartment of Virology, Faculty of Veterinary Medicine, Kafr El Sheikh University, EgyptS.KasemDepartment of Virology, Faculty of Veterinary Medicine, Kafr El Sheikh University, EgyptMagdaA. KaladDepartment of Virology, Faculty of Veterinary Medicine, Kafr El Sheikh University, EgyptJournal Article20200317The current study was planned to isolate and identify the Equine herpesvirus-1 (EHV-1) from liver, lung, spleen and fetal fluid, collected from five aborted fetuses of Arabian mares at the last trimester of pregnancy, with condensing on the 6th case of early embryonic death at two months of pregnancy. Aborted fetuses and paired sera sample of their dames were submitted from a private stud in Cairo, with history of recurrent abortion. An attempt for isolation of the causative agent was carried on Chorio-Allantoic membrane (CAM) of specific pathogen free- embryonated chicken eggs (SPF-ECE) and tissue culture (VERO cells).EHV-1 was isolated from 5 out of 6 aborted cases (No.6 is one of them) given a characteristic pock lesion and CPE with titer ranged from 8 -9.5 log10 EID50 at 3rd egg passage and 7 -8.5 log10 TCID50 at 5th cell culture passage. Isolated virus was identified by Virus Neutralization (VN) test, Fluorescent Antibody Technique (FAT) and Polymerase Chain Reaction (PCR). On the other hand there was more than four fold increase in the EHV-1 antibodies titer by Enzyme Linked Immune-Sorbent Assay (ELISA) in the convalescent sera sample which indicates recent infection by EHV-1.Beni-Suef University; Faculty of Veterinary MedicineJournal of Veterinary Medical Research2357-051222120130301The Hepatoprotective Effects of Vitamin C and Micronized Vitamin C against Paracetamol Induced Hepatotoxicity in Rats: A Comparative Study46527767810.21608/jvmr.2013.77678ENK. M. A.HassninDepartment of Biochemistry, Faculty of Veterinary Medicine, Beni-Suef Universit, Beni-Suef, EgyptK. S.HashemDepartment of Biochemistry, Faculty of Veterinary Medicine, Beni-Suef Universit, Beni-Suef, EgyptJournal Article20200317Micronization of chemical compounds is a new promising field as it reduces the size of the particles thus it increases their penetration power. Many theories prove that micronized particles are more effective even in very low concentration as compared to its normal size. Paracetamol is a commonly used effective analgesic and antipyretic agent for relief of mild and moderate pain. However, deliberate overdose or accidental overdose can cause hepatotoxicity. In the present study, we have evaluated and compared between the hepatoprotective and antioxidant effects of<br />vitamin C and micronized vitamin C against paracetamol induced hepatotoxicity in rats. Activities of liver enzymatic markers (Alanine amino transferase "ALT", Aspartate amino transferase "AST", Alkaline Phosphatase "ALP") and total protein "TP" concentration were estimated in serum. Lipid peroxidation "MDA" and antioxidant status (reduced glutathione "GSH " concentration, glutathione reductase "GR", catalse and super oxide dismutase "SOD" activities) were measured in tissue homogenates. Paracetamol administration (600 mg/ Kg B.wt.) significantly increased the liver enzymatic markers and decreased the total protein level. It also increased hepatic lipid peroxidation “MDA” and the activities of both catalase and SOD while it decreased GSH content and glutathione reductase (GR) activity. Treatment of vitamin C and micronized vtamin C restore the measured parameters nearly to their normal levels. Finally, micronized vitamin C has a more potent effect than ordinary vitamin C. Beni-Suef University; Faculty of Veterinary MedicineJournal of Veterinary Medical Research2357-051222120130301Molecular Typing of Clostridium perfringens Isolates from Soil, Healthy, and Diseased Sheep in Egypt by Multiplex PCR53577768010.21608/jvmr.2013.77680ENM.NayelDeptartment of Animal Medicine and Infectious DiseasesA.El-SifyDeptartment of Animal Medicine and Infectious DiseasesS.AkramDeptartment of Animal Medicine and Infectious DiseasesM.AllaamDeptartment of Animal Medicine and Infectious DiseasesE.AbdeenDeptartment of Bacteriology,
Mycology and Immunology, Faculty of Veterinary Medicine, Menoufia University (Sadat City
branch), Egypt.H.HassanDeptartment of Animal Medicine and Infectious DiseasesJournal Article20200317In this study multiplex PCR was used for typing of Clostridium perfringens isolates from soil, clinically healthy and diseased sheep. Clostridium perfringens was isolated from 41 out of 100 soil samples, 12 out of 100 clinically healthy sheep and 118 out of 200 sheep with enterotoxaemia signs. Genotyping of 41 isolates from soil indicated that 29 (70.73%) were type A, 3 (7.31%) were type B and 9 (21.95%) were type D. Of 12 isolates from clinically healthy sheep 6 (50%) were type A and 6<br />(50%) were type D. Of 118 isolates from diseased sheep 42 (35.59%) were type A, 22 (18.64%) were type B and 54 (45.76%) were type D. This result indicates that Clostridium perfringens type A, B and D are the main types causing enterotoxaemia in sheep in Egypt and Clostridium perfringens type A must be included in any vaccine programme to ensure optimum protection. Beni-Suef University; Faculty of Veterinary MedicineJournal of Veterinary Medical Research2357-051222120130301Bovine Neurobrucellosis: Pathological and Bacteriological Studies58637768110.21608/jvmr.2013.77681ENKh.A. El-NesrDepartment of Pathology, Faculty of Veterinary Medicine,
Beni-Suef University, Beni-Suef 62511, EgyptE. A.MahdiDepartment of Pathology, Faculty of Veterinary Medicine,
Beni-Suef University, Beni-Suef 62511, EgyptW. H.HamdiDepartment of Microbiology, Faculty of Veterinary Medicine,
Beni-Suef University, Beni-Suef 62511, EgyptA. H.AbedDepartment of Microbiology, Faculty of Veterinary Medicine,
Beni-Suef University, Beni-Suef 62511, EgyptJournal Article20200317Thirty two cattle, had no neurological syndrome, were serologically positive to brucellosis by using Tube agglutination and Rose Bengal Tests, by the official veterinary authority in Beni Suef province, Egypt. These animals were slaughtered in Beni Suef abattoirs during the project of control and eradication of brucella positive animals in Beni Suef province. Postmortem examination was performed and the brain was mechanically removed. Longitudinally the brain was cut; one half was fixed in formalin 25% for 2 weeks and the other one were sent to microbiology department for bacteriological isolation. Transverse sections were done in the fixed<br />tissue and samples were collected from cerebrum, cerebellum, medulla oblongata, thalamus, hypothalamus, and caudate nucleus. These samples were processed according to Bancroft and Gamble (2008). From the thirty two brain samples, no isolates of brucella species were recovered. From the thirty two brain samples, no isolates of brucella species were recovered. Brains of slaughtered animals showed no pathological lesions grossly. Microscopically, inflammatory reactions, degeneration, malacia, demyelination, pigmentation,and vascular changes were detected.Beni-Suef University; Faculty of Veterinary MedicineJournal of Veterinary Medical Research2357-051222120130301Effect of some neurotransmitters on pituitary in bull-camel64707768210.21608/jvmr.2013.77682ENS.S.IbrahimDepartment of Physiology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511,
EgyptA.Aboul-ElaDepartment of Physiology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511,
EgyptE.AMabroukDepartment of Physiology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511,
EgyptT.El-MetenawyNational Research Center, Dokki, Egypt.FatenAbo-AzizaNational Research Center, Dokki, Egypt.Journal Article20200317Effect of some neurotransmitters on pituitary in bull-camelThe present in vitro study is an attempt for profound exploration of the influence of some inhibitory (GABA and taurine) and excitatory neurotransmitters (glutamic acid and L-Argnine) on pituitary gland gonadotropic hormones in camels. For this purpose, neurotransmitters were added individually to pituitary cells in vitro. Results of current study revealed that addition of GABA alone has a potent inhibitory influence on gonadotropins release; an action which was dosedependent. In addition, exaggerated high dose of GABA added to culture resulting in depression of GnRH activity. Inhibitory neurotransmitter taurine preserved gonadotropins release within their normal values and maintained the stimulatory potentials of GnRH. On the other side, nitric oxide had a stimulatory action at small dose rates and a depressive effect at higher dose rates. Glutamic acid as the major endogenous excitatory neurotransmitter had a potent dose-dependent excitatory effect upon FSH and LH as well as GnRH stimulatory influence. Beni-Suef University; Faculty of Veterinary MedicineJournal of Veterinary Medical Research2357-051222120130301The effect of Rabies Infection and Vaccination on Pregnancy in Rats as Animal Model77807768310.21608/jvmr.2013.77683ENAmani,A. SalehVeterinary Serum and Vaccine Research Institute, Abassia, Cairo.A. F.SolimanVeterinary Serum and Vaccine Research Institute, Abassia, CairoA. M.AlbehwarVeterinary Serum and Vaccine Research Institute, Abassia, Cairo.M. B.ShendyVeterinary Serum and Vaccine Research Institute, Abassia, Cairo.Journal Article20200317The effect of rabies infection and vaccination on pregnancy was investigated in different groups of pregnant rats as an animal model. Intracerebral and intramuscular experimental infection with CVS rabies virus strain was applied on four pregnant rats groups at the middle (seven days after mating) and late stages of gestation (14 days after mating). Subcutaneous rout vaccination of other three pregnant rat groups five to seven days before; seven and 14 days after mating with the inactivated cell culture local rabies vaccine. Each group of infected rats showed clinical signs of rabies although their fetuses did not show any abnormalities. Virus recovery from the placenta and fetuses from dead and sacrificed animals failed to induce rabies signs in mice inoculated intracerebrally with placenta and fetus suspensions while brains of infected dams; through the routes; revealed positive FA by using fluorescent antibody technique. Vaccinated pregnant rats did not show any abnormalities with normal fetuses and good levels of specific rabies antibodies when estimated by serum neutralization test. These findings indicate that rabies vaccination of pregnant animals is safe and it could be recommended to protect both of dams and their offspring in the first months.Beni-Suef University; Faculty of Veterinary MedicineJournal of Veterinary Medical Research2357-051222120130301The Hypolipdemic and Antioxidant Effects of Porobiotic, Prebiotics and Natural Lipotropic Factors on Hyperlipidemia and Fatty Liver in Broilers81907768510.21608/jvmr.2013.77685ENDoaaS. MohamedDepartment of Biochemistry, Faculty of Veterinary
Medicine, Beni-Suef University, Beni-Suef 62511, Egypt.M. F.El KadyDepartment of Poultry Diseases, Faculty of Veterinary
Medicine, Beni-Suef University, Beni-Suef 62511, Egypt.K. M.HassaninDepartment of Biochemistry, Faculty of Veterinary
Medicine, Beni-Suef University, Beni-Suef 62511, Egypt.K. S.HashemDepartment of Biochemistry, Faculty of Veterinary
Medicine, Beni-Suef University, Beni-Suef 62511, Egypt.Journal Article20200317Fatty liver is considered a serious problem in broilers as liver is the main organ involved in metabolism of all absorbed substances. In addition, fatty liver has a direct impact on the growth and performance of the chicken. One of the modern approach in controlling metabolic upset, is the use of probiotics, prebiotics in controlling this problems. Here we tried to summarize the protective role of some probiotics, prebiotics and other natural lipotropic factors and their mode of action against hperlipidemia and fatty liver in broilers.Beni-Suef University; Faculty of Veterinary MedicineJournal of Veterinary Medical Research2357-051222120130301Preliminary Investigation on Buxtonella sulcata (Jameson, 1926) (Ciliphora: Trichostomatidae) in Egyptian Ruminants91947768710.21608/jvmr.2013.77687ENK.SultanDepartment of Parasitology, Faculty of Veterinary Medicine, Kafr El-Sheikh University, EgyptR. E.,KhalafallaDepartment of Parasitology, Faculty of Veterinary Medicine, Kafr El-Sheikh University, EgyptM. A.ElseifyDepartment of Parasitology, Faculty of Veterinary Medicine, Kafr El-Sheikh University, EgyptJournal Article20200317Buxtonella sulcata (Jameson, 1926) is an intestinal protozoan of large ruminants, with scanty information and contradictious reports about its pathogenesity. This work aimed to investigate the prevalence rate of B. sulcata in Egypt. Forty eight cases collected from cattle (n= 29) and buffaloes (n= 19) from El-Mahalla El-Kubra area, Al-Gharbiya province. Samples were examined for the presence B. sulcata. The overall infection rate was 41.6% (20/48), in cattle 48.2% (14/29) and in buffalo 31.5% (6/19). This is the first study on B. sulcata in Egyptian ruminants also highlights the situation of intestinal ciliates of ruminant animals and provides basic information for the future work of intestinal ciliates of animals and man Beni-Suef University; Faculty of Veterinary MedicineJournal of Veterinary Medical Research2357-051222120130301Seroprevalence of Ovine Brucellosis in the Western, Middle and Southern Areas of Libya95987768810.21608/jvmr.2013.77688ENM.Abo RokiaFaculty of Agriculture and Veterinary Science, Tarhouna, AL Mergeb UniversityL.BakarFaculty of
Veterinary Medicine and Agriculture Science, El- Zawia University.A. A.AbdallaFaculty of
Veterinary Medicine and Agriculture Science, El- Zawia UniversityJournal Article20200317In this study a total of 2230 sheep (one-three years of age) were serologically surveyed in three selected areas in Libya (Western, Middle and Southern areas) to specify foci of infection and determination of the prevalence of ovine brucellosis using Rose Bengal Plate Test and Rivanol test. Prevalence of brucellosis in this study revealed 4%, 0%and 0%, respectively. Only the western area showed positive cases, while the Middle and Southern areas showed no serological evidence of brucella infection.Beni-Suef University; Faculty of Veterinary MedicineJournal of Veterinary Medical Research2357-051222120130301Physiological Response to the Stress Effect of Anesthesia and Surgery in Sheep and Goat991047768910.21608/jvmr.2013.77689ENM.Abdel FattahDepartmentof Surgery, Anesthesia and Radiology, Faculty of
Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt.NermeenA. HelmyDeptartment of Physiology, Faculty of
Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt.S. S.IbrahimDeptartment of Physiology, Faculty of
Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt.Journal Article20200317The present study was established to evaluate and compare stress response to anesthesia as well as surgery in sheep and goat. For this purpose, 5 rams and 5 bucks underwent anesthesia alone followed a week later by anesthesia with rumenotomy. Serum cortisol levels as well as differential leucocytic count were assayed just prior to anesthesia (0 time) and then after 1, 2, 4, 6 and 24 hours post anesthesia. In the second experiment, blood samples were collected just prior to anesthesia (0 time) then after half an hour (0 time post rumenotomy) then after 1, 2, 4, 6 and 24 hours post completion of rumenotomy. Serum cortisol levels in both species increased significantly and reach its peak one hour following anesthesia (3.31 ± 0.17 and 4.09 ± 0.12, respectively) which were higher than subsequent cortisol levels. In addition, Peak cortisol level in sheep obtained at 0 time post rumenotomy (4.39 ± 0.13) and 1 hour following rumenotomy in goat (3.45 ± 0.16). The significant increase in cortisol level persists for 6 hours and returns to normal level 24 hours following surgery. Beni-Suef University; Faculty of Veterinary MedicineJournal of Veterinary Medical Research2357-051222120130301Comparison of Adjuvant Efficacy of Different Molecular Weights of Chitosan and Aluminum Hydroxide for Inactivated Rabies Vaccine1051117769110.21608/jvmr.2013.77691ENAmaniA. SalehVeterinary Serum and Vaccine Research Institute; Abassia, CairoJournal Article20200317The development of safe, novel adjuvant is necessary to maximize the efficacy of new and/or available vaccines. In this study, three different molecular weights of chitosan (low, medium, high) were evaluated as immunopotentiators/adjuvants of inactivated rabies vaccine in white Norway rats as an experimental model. Two concentrations (1.5% & 3% w/v) of each chitosan type were used in final concentration 5 mg/ml and 10 mg/ml. Results showed that lymphocyte proliferation<br />were significantly elevated (P < 0.05) in all chitosan vaccinated rats compared to aluminum hydroxide gel vaccinated one. All three type of chitosan produced accelerated and enhanced effects on rabies-neutralizing antibody responses in vaccinated groups. Increases in antibody titers together with lymphocytes proliferation responses revealed that chitosan induced both humoral and cell-mediated immune responses. When compared with aluminum hydroxide vaccine<br />adjuvant, chitosan was superior to aluminum hydroxide. The conclusion of these results suggested that chitosan with different MW had a strong potential to increase both cellular and humoral immune responses and that chitosan may be a promising and efficacious adjuvant candidate suitable for inactivated rabies vaccine.Beni-Suef University; Faculty of Veterinary MedicineJournal of Veterinary Medical Research2357-051222120130301Attenuation effect of UV on Haemonchus contortus Larvae in Experimentally Infected Goats1121207769210.21608/jvmr.2013.77692ENS. M.AboelhadidDepartment of Parasitology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef
62511, EgyptM. A.El-AskalanyDepartment of Parasitology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef
62511, EgyptW. M.MousaDepartment of Parasitology, Faculty of Veterinar Medicine, Cairo University,
Cairo, EgyptW. M.ArafaDepartment of Parasitology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef
62511, EgyptD.PiedrifitaDepartment of Physiology, Faculty of Veterinary Medicine, Monash University,
Australia.Journal Article20200317This study was conducted to study the UV protective effect against Haemonchus contortus infection in goats. Sixteen male goats were divided into 5 groups, control infected, control uninfected and UV 30minutes; UV 60minutes and UV booster 60minutes exposure. The UV groups were exposed to UV irradiation at wave length 254nm for 30 and 60 minutes. The UV booster 60min was administrated 2 doses of exposed larvae with an interval of one month. All groups except the control negative one were challenged for 42 days from the beginning. In UV booster<br />60min had reduction in egg count per gram feces and worm burden (93% & 34 % respectively). These parameters were similar in control infected, UV 30min and UV 60min groups. Increases in levels of antibodies were found in goats of UV booster 60min group the other groups. Finally, 2 doses of UV 60min exposure could protect goats from H. contortus.Beni-Suef University; Faculty of Veterinary MedicineJournal of Veterinary Medical Research2357-051222120130301Investigation of Enteric Parasites of Zoo Animals and Zookeepers in Beni-Suef Governorate, Egypt1211257769310.21608/jvmr.2013.77693ENW. M.ArafaDepartment of Parasitolog, Faculty of Veterinary Medicine, Beni-Suef Uineveristy. Beni-Suef, Egypt.LilianN. MahrousDepartment of Parasitolog, Faculty of Veterinary Medicine, Beni-Suef Uineveristy. Beni-Suef, Egypt.S. M.AboelhadidDepartment of Parasitolog, Faculty of Veterinary Medicine, Beni-Suef Uineveristy. Beni-Suef, Egypt.A. E.Abdel-GhanyDepartment of Animal Hygiene, Management and Zoonoses,
Faculty of Veterinary Medicine, Beni-Suef Uineveristy. Beni-Suef, Egypt.Journal Article20200317In this study, the enteric parasites of zoo animals and zookeepers in Beni-Suef governorate, Egypt were investigated. Fecal samples from thirteen animal species were examined by floatation and formol ether sedimentation techniques. Zoo animals were classified into non-human primates, carnivores and herbivorous animals. The examination of non-human primates revealed the presence of Trichuris trichura eggs, Giardia intestinals and Entamoeba histolytica cysts. In carnivores, Toxoascaris leonina eggs and Isospora felis oocysts were the most predominant<br />findings. In herbivore wild animals, gastrointestinal nematode (GIT) eggs and Eimeria species oocysts were present. Larval identification by fecal culture of (GIT) eggs demonstrated the presence of Haemonchus contortus and Strongyloid papillosus larvae. Examination of zookeepers and one lab worker revealed the presence of Giardia intestinals and Entamoeba histolytica cysts. In conclusion, infection with Giardia intestinals and Entamoeba histolytica in both of human and nonhuman<br />primates suggests the zoonotic transmission in the zoo. Beni-Suef University; Faculty of Veterinary MedicineJournal of Veterinary Medical Research2357-051222120130301Sero-diagnosis of Bovine Tuberculosis by ELISA Using Bovine PPD and ST.CF1261297769410.21608/jvmr.2013.77694ENA.El-SifyDepartment of Animal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Sadat City
Branch, Minufyia Univeristy, EgyptM.NayelDepartment of Animal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Sadat City
Branch, Minufyia Univeristy, EgyptS.HazemDepartement of Immunology and Immunopharmacolgy, Animal
Reproduction Research Institution, EgyptR.TarabessDepartment of Bacteriology, Mycology and Immunology
Faculty of Veterinary Medicine, Sadat City Branch, Minufyia Univeristy, EgyptS.AkramDepartment of Animal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Sadat City
Branch, Minufyia Univeristy, EgyptM.AllaamDepartment of Animal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Sadat City
Branch, Minufyia Univeristy, EgyptH.HassanDepartment of Animal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Sadat City
Branch, Minufyia Univeristy, EgyptM.El GarhyAgriculture
Extension Sector, Ministry of Agriculture, EgyptJournal Article20200317Bovine tuberculosis represents one of the very important infectious diseases in Egypt and the world. It has zoonotic importance and causes severe economic losses. Accurate and rapid diagnosis considered as the milestone for control of the disease. In this study ELISA technique was used for confirmation of positive reactors cows that tested with single intradermal tuberculin test, to detect false positive reactors. Bovine PPD and ST.CF antigens have been used as two different coating<br />antigens for ELISA technique. 3747 cattle from dairy farms in five different governorates were subjected to the single intradermal cervical tuberculin test whereas 78 (2.24%) proved positive reactors to tuberculin. These positive reactors tested with ELISA. 64 (82.05%) animals were positive by ELISA coated with ST-CF, while by using bovine PPD as coating antigen 58 (74.35%) animals were positive. The previous results indicated that ELISA test showed higher sensitivity and specificity using ST-CF as coating antigen than in case of bovine PPD coating antigen. Beni-Suef University; Faculty of Veterinary MedicineJournal of Veterinary Medical Research2357-051222120130301Occurrence of Biogenic Amines in Different Types of Marketed Cheese in Gharbia Governorate1301357769510.21608/jvmr.2013.77695ENNesreenZ. Eleiwanimal Health Research Institute, Tanta Lab and 2Animal Health Research Institute, Benha Lab.HananM. LamadaAnimal Health Research Institute, Tanta Lab and 2Animal Health Research Institute, Benha LabMarionetteZ. NassifAnimal Health Research Institute, Tanta Lab and 2Animal Health Research Institute, Benha LabJournal Article20200317This work was applied on sixty cheese samples represented by Kareish ,cheddar and Romano cheeses (20 of each).The samples were submitted to High Performance Liquid Chromatography (HPLC) for qualitative and quantitative determination of biogenic amines .The results were summarized as Kareish cheese has higher concentrations of Tyramine and Cadaverine in low and high levels of manufacturing quality ( 29.64 ± 1.72 and 9.91 ± 0.60 mg/100gm) and (17.48 ± 1.09 and 5.61± 0.37 mg/100gm) respectively, Meanwhile, Histamine level was higher in both levels of Romano cheese (22.96 ± 1.17 and 18.35± 1.12 mg/100gm) respectively. Putrescine represented in high levels in cheddar cheese (13.40 ± 1.02 and 10.61 ± 0.74 mg/100gm) respectively. Comparing with the Egyptian Organization for Standardization"EOS" (1996), all the cheese samples were not exceeded the permissible level of Cadaverine in contrast with the other biogenic amines. The study<br />concluded that presence of high concentrations of biogenic amines reflect the bad hygienic conditions under which they produced and stored Beni-Suef University; Faculty of Veterinary MedicineJournal of Veterinary Medical Research2357-051222120130301Studies on the Prevalence of Enterobacteriaceae in Chickens and Chicken eggs1361447769610.21608/jvmr.2013.77696ENM. M.AmerDepartment of Poultry Diseases, Faculty of Veterinary Medicine, Cairo UniversityA. H. M.DahshanDepartment
of Poultry Diseases, Faculty of Veterinary Medicine, Beni-Suef UniversityHalaS. HassanDepartment of
Bacteriology and Mycology, Faculty of Veterinary Medicine, Beni-Suef UniversityAsmaaA. MohamedVeterinary
Supervisor, Animal Production, Faculty of Agriculture, Al-Minia UniversityJournal Article20200317This study was done to investigate the prevalence of the Enterobacteriaceae in chickens and eggs. Isolation of forty four different bacterial isolates belonging to Enterobacteriaceae from chicken egg samples, cloacal swabs and swabs from Hatcheries’s floor, the isolates from commercial flock swabs were biochemically identified as E coli, P. mirabilis E Sakazakii and E .cloacae by incidence 22%, 55 %, 11% and 11 % respectively. The isolates from Layers and broilers breeder cloacal swabs were biochemically identified to be E. coli, P. mirabilis E. fergusonii and E .cloacae by incidence 20 %, 20 %, 20% and 40 % respectively. The isolates from commercial eggs were biochemically identified to be Pantoea Sp. , Kluyvera sp., E Sakazakii , E.aerogenes and E.harmanii by incidence 33.3% , 16.6% , 16.6% , 16.6% and 16.6 % respectively. The isolates from fertilized egg samples were biochemically identified as E Sakazakii , E. fergusonii , E.coli , E. Cloacae , Aeromonas ,S. Anatum and Prov. Alcolifaciens with a number of 1 ,1, 3, 3, 2, 2 and 1 , incidence 8% , 8% , 23% , 23% , 15% , 15% and 8 % respectively. The incidence of Enterobacteriaceae isolates from floor swabs of both primitive and automatic hatcheries was 20.8 % and these isolates were biochemically identified to be Pantoea spp., Citrokoserilama, k.pneumo. Ozaenae and E .cloacae with number 2, 1, 1 and 1 also its incidence were 40%, 20%, 20% and 20 % respectively. We found that the most common isolated bacterium from eggs either fertilized or<br />commercial table eggs in our study was E.coli although we could isolate other bacterial species as Enterobacter, Proteus species , Escherichia fergusonii; E. Sakazakii, Klebsiella sp., S. anatum, and Pseudomonas sp..In-vitro sensitivity test of the isolated strains to various chemotherapeutic agents revealed that all isolates were sensitive to Ciprofloxacin, Enrofloxacin, and Amoxicillin. Beni-Suef University; Faculty of Veterinary MedicineJournal of Veterinary Medical Research2357-051222120130301Clinco-Pathological and Biochemical Studies on Sheep Fed Aflatoxin Contaminated Ration1451537769710.21608/jvmr.2013.77697ENRandaA. HassanPathological Department Animal Health Research Institute, Dokki,
Egypt.M. M.ArafaBiochemical Department Animal Health Research Institute, Dokki,
Egypt.Journal Article20200317Fifty sheep from Suez Governorate were suffered from high mortality rate (10% in the herd), weakness, depression, congestion of all mucous membrane and intestinal diarrhea. Ration analysis revealed presence of aflatoxin B1 , contamination (300 ppb). Hemogram, biochemical and histopathological examination demonstrated. Hemogram in aflatoxicated sheep showed significant decrease in red blood corpuscles (RBCs) count, Hemoglobin % and pack cell volume (PVC). Normocytic norm chronic anemia as well as leucopenia as compared to control values.<br />Hypoprotenemia, hypoalbumin, elevated liver and kidney enzyme were observed. Also, there was decrease in serum electrolyte (Ca, P, Mg, Na, K) as result of decrease re-absorption from the inflamed renal tubules. Also, demonstrated high residue of aflatoxin in liver, kidneys and muscles of affected sheep compared with normal one. Pathological examination of internal organs revealed severe damage in lung, heart, liver, kidney and intestine, that aflatoxins induced severe hepatic,<br />renal and cardiac lesions which cause mortalities. Beni-Suef University; Faculty of Veterinary MedicineJournal of Veterinary Medical Research2357-051222120130301Ethological Problems and Learning Disability due to Aluminium Toxicity in Rats1541597769810.21608/jvmr.2013.77698ENAmira,A. GomaDepartment of Animal Husbandry and wealth development, Faculty of Veterinary Medicine,
Alexandria University, EgyptU. E.MahrousDepartment of Animal Husbandry and wealth development,
Faculty of Veterinary Medicine, Damanhour University, EgyptJournal Article20200317A total of 35 Sprague-Dawley adult rats were used to investigate the effect of aluminium toxicity on behavioural patterns of adult female rats and learning ability of offspring. Rats were allotted into 4 groups, group one received 2g/l anhydrous aluminium chloride (n=10), group two received 3g/l anhydrous aluminium chloride (n=10), group three received 3.5g/l anhydrous aluminium chloride in drinking water (n=10) and control group did not receive anhydrous aluminium chloride (n=5) from 8th day of pregnancy till weaning of pups. The obtained results showed that feeding time increased significantly in 2g/l and 3.5g/l anhydrous aluminium chloride groups than control one, while, litter licking frequency and nursing time increased significantly in 2g/l anhydrous aluminium chloride than other groups. On contrary<br />lying time decreased significantly in rats treated with 2g/l anhydrous aluminium chloride than other groups, licking and scratching decreased in 3g/l and 3.5g/l anhydrous aluminium chloride groups. In considering, the time spent in closed arms by offspring pups exhibited much times significantly than control group, while, time spent in open arms of elevated plus maze decreased significantly in all treated groups than control group. On the other hand, number of entries in open arms significantly decreased in treated groups than control one.Beni-Suef University; Faculty of Veterinary MedicineJournal of Veterinary Medical Research2357-051222120130301Monitoring of the Immune Response of Pregnant Ewes and their Offsprings Following the Vaccination of the Dams with Peste Des Petits Ruminants Attenuated Vaccine (75/1)1601637769910.21608/jvmr.2013.77699ENHussein,A. H. M.Veterinary Serum and Vaccine Research Institute, Abbasia, CairoHananM.S. El-ZawahryVeterinary Serum and Vaccine Research Institute, Abbasia, CairoAbeerA. TammamVeterinary Serum and Vaccine Research Institute, Abbasia, CairoJournal Article20200317Assessment of the neutralizing antibody response to the attenuated strain of peste des petits ruminants Nigerian strain (75/1) in 5 pregnant ewes revealed a geometric mean titre of 194, four weeks post Vaccination. Such an appreciably high titre was found to be dropped to 73.5, throughout 48 hours post parturn. Suckling kids born to these dams passively acquired a titre of 84.5 throughout their first month of life that dropped to 48.5 at the age of 2 months. At their 5th month of age their immune titre was found to be only 4. Data generated from this study might be<br />of value in launching vaccination campaigns against peste des petits ruminants disease. Beni-Suef University; Faculty of Veterinary MedicineJournal of Veterinary Medical Research2357-051222120130301An epidemiological study on giardiasis in cattle and humans at Beni-Suef Governorate1641698018910.21608/jvmr.2013.80189ENGihan K.Abdel-LatifDepartment of Animal Hygiene, Management and Zoonoses, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt.AboelhadidS. M.Department of Parasitology,
Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt.Journal Article20200402<strong>The present study was conducted to assess the prevalence of </strong>Giardia <strong>species infection in cattle</strong><br /><strong>and human. One hundred of animal fecal samples and 139 human stool samples were collected</strong><br /><strong>from different veterinary clinics and its related hospitals respectively. All samples were undergone</strong><br /><strong>to microscopically examination by; direct smears in 0.90% Na Cl solution, Lugol's iodine stain for</strong><br /><strong>cyst detection and formol-ether concentration. 9 (28.1%) calves from 32 were positive in</strong><br /><strong>microscopic examination by the used techniques. 25% of the examined fecal samples of cattle</strong><br /><strong>(17/68) were containing cysts of </strong>Giardia <strong>species by microscope. 39 of 139 (28.1%) of human stool</strong><br /><strong>samples were found infected by this protozoon. Regarding the sex of human cases, 26.30% of</strong><br /><strong>examined males were positive while 30.20% of females were positive. The age factor in human</strong><br />infection was clear; the age group of 11 to 20 years were the more infected than the other group <strong>(1-</strong><br /><strong>10ys). There is no relation between form of human stool and infection rate. ELISA kits confirmed</strong><br /><strong>that 6 % of animal cases and 15.8% of human were positive. The epidemiological aspects were</strong><br /><strong>discussed in the study.</strong>