Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
27
2
2020
12
01
Effect of Platelet-rich Plasma on MMP-13, ARE and TGF β1 in MIA-Induced Osteoarthritis in Rats
109
118
EN
Fatma
M. Halfaya
Surgery, Anesthesiology and Radiology Department, Faculty of Veterinary Medicine, Beni Suef University, Egypt
fma_vet2010@yahoo.com
Ragab
G.H.
Surgery, Anesthesiology and Radiology Department, Faculty of Veterinary Medicine, Beni Suef University, Egypt
prof.gamalhassan@yahoo.com
Hagag
U.
Surgery, Anesthesiology and Radiology Department, Faculty of Veterinary Medicine, Beni Suef University, Egypt
usama_hagag2006@yahoo.com
Ahmed
O.M.
Physiology Division, Zoology Department, Faculty of Science, Egypt
osama.ahmed@science.bsu.edu.eg
Abou Elkheir
W.
Department of Immunology, Military Medical Academy, Cairo, Egypt
dr.wael2008@yahoo.com
10.21608/jvmr.2020.34762.1019
Ankle osteoarthritis (OA) is an inflammatory deterioration chronic disease; yet, OA pathogenesis is obscure. There is no absolute cure from OA and the present pharmacological medication options are constrained and associated with adverse aspect effects. Clinically, Platelet-rich plasma (PRP) is usually used to cure OA. The present study was designed to evaluate the role of PRP in the treatment of experimentally Monosodium iodoacetate (MIA)-induced ankle osteoarthritis in the rat model. Thirty male Wistar rats were divided into three groups (each of 10 rats). Rats of group I were injected with 1 mg MIA in the right ankle joint for two consecutive days, while those of group II were treated with saline instead of MIA; and group III (osteoarthritic +PRP) rats were injected with PRP in the ankle joint at 14, 21, and 28 days after MIA injection. Paw oedema, scoring of arthritis, Matrix metalloproteinase 13 (MMP13) level, antioxidant response element (ARE) level, and joint transforming growth factor beta1 (TGF β 1) were evaluated. PRP reduces expression of joint, MMP13, ARE level, and TGF β 1. PRP also significantly reduces the score of arthritis. The administration of PRP decreases paw oedema in MIA-induced OA rats. These results suggest that PRP has marked effect against OA in MIA-induced osteoarthritic rats which are mediated through the anti-inflammatory and antioxidant effects.
ARE induced Osteoarthritis,MMP-13,platelet-rich plasma,TGF β1
https://jvmr.journals.ekb.eg/article_110640.html
https://jvmr.journals.ekb.eg/article_110640_d13199675e55f6b0ff6356d953f929a1.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
27
2
2020
12
01
Efficacy of Hyaluronic Acid in the Treatment of MIA-induced Ankle Osteoarthritis in Rats and its Effect on Antioxidant Response Element
119
127
EN
Fatma
M. Halfaya
Surgery, Anesthesiology and Radiology Department, Faculty of Veterinary Medicine, Beni Suef University, Egypt
fma_vet2010@yahoo.com
Ragab
G.H.
Surgery, Anesthesiology and Radiology Department, Faculty of Veterinary Medicine, Beni Suef University, Egypt
prof.gamalhassan@yahoo.com
Hagag
U.
Surgery, Anesthesiology and Radiology Department, Faculty of Veterinary Medicine, Beni Suef University, Egypt
usama_hagag2006@yahoo.com
Ahmed
O.M.
Physiology Division, Zoology Department, Faculty of Science, Egypt
osama.ahmed@science.bsu.edu.eg
Abou Elkheir
W.
Department of Immunology, Military Medical Academy, Cairo, Egypt
dr.wael2008@yahoo.com
10.21608/jvmr.2020.34766.1020
Ankle osteoarthritis (OA) is a long-standing inflammatory degeneration disease; until now, its pathogenesis remains ambiguous. There is no complete remedy from OA and the present pharmacological therapy choices are restrained and combined with undesirable side effects. Clinically, Hyaluronic acid (HA) is widely consumed to cure OA. The present experiment aimed to assess the role of HA in the remedy of experimentally Monosodium iodoacetate (MIA) -induced ankle OA in the rat model. Thirty male Wistar rats were divided into 3 groups (each of 10 rats). Rats of group I were injected with 1 mg MIA in the right ankle joint for two successive days, while those of group II were treated with saline instead of MIA; and group III( osteoarthritic + HA) rats were injected with HA in the ankle joint at 2nd, 3rd, and 4th weeks following injection of MIA. Bodyweight, ankle measurement, total leukocytes count (TLC), antioxidant response element (ARE) level, and joint Magnetic Resonance Imaging (MRI) were investigated. HA reduced expressions of joint ARE level. HA also markedly reduced the TLC. The administration of HA decreases ankle measurement in MIA-induced OA rats. MRI of HA showed a gradual reduction in joint damage. These results suggest that HA has improvement effects on OA rats which are assessed through anti-inflammatory and antioxidant effects.
ankle osteoarthritis,Antioxidant Response Element,Hyaluronic acid
https://jvmr.journals.ekb.eg/article_110641.html
https://jvmr.journals.ekb.eg/article_110641_9637b7284d027144a35cf7fe8ae619d3.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
27
2
2020
12
01
Molecular Characterization of Antimicrobial-resistant Escherichia coli Isolated from Broiler Chickens
128
142
EN
Ismail
Radwan
Bacteriology, Mycology and Immunology Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt
microbiologist111@yahoo.com
Mohammed
Abd El-Halim
El-Minia Directorate of Veterinary Medicine, Egypt
seifmwa@gmail.com
Ahmed
Hussein
Abed
0000-0002-7221-6340
Bacteriology, Mycology and Immunology Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt
aboabedelmasry@yahoo.com
10.21608/jvmr.2020.31870.1009
<span>Avian colibacillosis is one of the most important diseases of chickens resulting in significant economic losses as well as high morbidity and mortality. In the current study, the prevalence of avian colibacillosis was studied in different farms of broiler chickens in Beni-Suef, EL-Minia, ElFayoum, Assiut and Sohag Governorates. A total of 300 pooling samples were collected aseptically from heart blood as well as the affected organs including air sacs, pericardial sac, and liver of slaughtered diseased and freshly dead broiler chickens. Bacteriological examination of the collected samples showed that a total number of 80 E. coli isolates were recovered with an overall prevalence of 26.7. The highest prevalence was recorded in El-Fayoum (33.3%) followed by El-Minia (25%), Beni-Suef and Assiut (22.5% for each) while the lowest prevalence was in Sohag (17.5%). The invitro antimicrobial susceptibility testing revealed that E. coli isolates showed high sensitivity to colistin sulphate only (70%). Meanwhile, high resistances were recorded against other antimicrobials including amoxicillin (97.5%), cefotaxime sodium and florfenicol (95% for each), apramycin, ciprofloxacin and gentamicin (92.5% for each), streptomycin (90%), enrofloxacin (87.5%), trimethoprim-sulphamethoxazol and doxycycline HCl (77.5% for each). All E. coli isolates were MDR (100%). PCR was applied on 10 MDR E. coli isolates to detect the 3 resistance-associated genes (qnrA, tetA and aac(6′)- Ib-cr and 5 virulence-associated genes (iutA, hly, sta, lt and astA). The results showed that all the tested isolates (100%) harbored qnrA, tetA, iutA and astA genes meanwhile aac(6′)-Ib-cr, hly, sta and lt genes were not detected in any isolate.</span>
Broiler chickens,E. coli,Multidrug resistance (MDR),resistance genes
https://jvmr.journals.ekb.eg/article_111375.html
https://jvmr.journals.ekb.eg/article_111375_117b96d68af2177c6930cd0c1c9319f2.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
27
2
2020
12
01
Evaluation of the Bactericidal Effect of Silver Nanoparticles Against Methicillin Resistant Staphylococcus aureus (MRSA) and Methicillin Sensitive Staphylococcus aureus (MSSA) Strains Isolated from Mastitic Milk of Small Ruminants and their Surrounding Environment in Aswan, Egypt
143
151
EN
Randa
Salah
Animal Health Department, Faculty of Veterinary Medicine, Aswan University, Egypt
randasalah174@gmail.com
Mohamed
Karmy
Department of Food Hygiene and Control, Faculty of Veterinary Medicine, Aswan University, Egypt
kearmy99@yahoo.com
Aml
Abdelraouf
0000-0002-0640-6779
Department of Infectious Diseases, Faculty of Veterinary Medicine, Aswan University, Aswan, Egypt
aml.raouf@yahoo.com
Saber
Kotb
Department of Animal and amp; Poultry Hygiene and Environmental Sanitation, Faculty of Veterinary Medicine, Assiut University, Egypt
saberkotb@yahoo.com
10.21608/jvmr.2021.55209.1027
In the recent years, emergence of infectious diseases caused by drug resistant pathogens had been increased; therefore there is an urgent need to search for new alternative and effective antimicrobial agents to overcome the drug resistance. In the present investigation, the study group consisted of 90 sheep and 90 goats with clinical evidences of mastitis in 17(18.89%) goats and 5 sheep (5.56%) that manifested swollen udder with or without systemic signs of illness related to mastitis. Standard bacteriology was performed on pretreatment milk samples from the 17 goats and 5 mastitic ewes as well as 60 soil samples and 60 pail water samples. The bacteria isolated were identified as Staphylococcus aureus 12 (70.6%) from goats and 5 (100%) from sheep. In addition, S. aureus could also be identified in 41 (68.3%) soil samples and 42 (70%) water samples. The current study aimed to explore the bactericidal effect of silver nanoparticles (AgNPs) on methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-sensitive Staphylococcus aureus (MSSA) strains isolated from mastitic milk and their surrounding environment (water and soil) in Aswan, Egypt. AgNPs solution was synthesized by typical one-step synthesis protocol using soluble starch and was characterized using transmission electron microscopy and atomic absorption spectrophotometer. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of AgNPs were examined to evaluate the bactericidal efficiency. In the present study, no significant discrepancy was observed between the influence of AgNPs on MRSA and MSSA (P> 0.05). It can be concluded that Ag-NPs have strong bactericidal effect against both MRSA and MSSA strains.
MBC,MIC,MRSA,MSSA,Silver nanoparticles (AgNPs)
https://jvmr.journals.ekb.eg/article_142741.html
https://jvmr.journals.ekb.eg/article_142741_c407a90703d466aeed48ab5ddec1aee6.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
27
2
2020
12
01
Prevalence, Characterization, and Control of Staphylococcus aureus Isolated from Raw Milk and Egyptian Soft Cheese
152
160
EN
Mohamed
Zeinhom
0000-0002-0811-9941
Food Hygiene and Control Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt
m.zeinhom@vet.bsu.edu.eg
Ahmed
Abed
0000-0002-7221-6340
Bacteriology, Mycology and Immunology Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt
aboabedelmasry@yahoo.com
10.21608/jvmr.2021.146885
<span>The present study aimed to detect the prevalence of <em>Staphylococcus aureus</em> <span lang="AR-SA" dir="RTL">)</span><em>S. aureus</em><span lang="AR-SA" dir="RTL">(</span> in raw milk, and soft cheese samples in Beni-Suef Governorate, Egypt, and to characterize some resistance and virulence related genes in the recovered S. aureus isolates. An additional objective was to evaluate the effectiveness of thyme oil for controlling S. aureus in cheese. A total of 200 samples of raw milk and cheese, including 100 samples of raw milk and 100 samples of two different types of cheese were used. S. aureus was isolated from 12.5% (25/200) of the raw milk and cheese samples. The highest prevalence was detected in Kareish cheese (18%), followed by raw milk samples (13%) and Talaga cheese (6%). S. aureus isolates showed high resistance to ampicillin (72%), and tetracycline (60%). PCR was applied on 6 multidrug resistant (MDR) S. aureus for detection of mecA and icaA genes which were detected in 66.7% and 33.3% of S. aureus isolates, respectively. As milk and dairy products are good substrates for S. aureus that causes staphylococcal food poisoning, reducing the level of such pathogen in milk and dairy products will lower the risk to consumers. Thyme oil 1% showed moderate inhibition against S. aureus, while using thyme oil 2% showed significant inhibition of <em>S. aureus</em> in Kareish cheese. Therefore, the use of thyme oil in preserving white soft Kareish cheese might be a promising approach to increase the shelf life and safety of the cheese.</span>
Antimicrobial resistance,cheese,control,Raw milk,S. aureus,Thyme oil
https://jvmr.journals.ekb.eg/article_146885.html
https://jvmr.journals.ekb.eg/article_146885_55780eb920f4982668bfdf49d27cb844.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
27
2
2020
12
01
Prevalence of Escherichia coli in Milk and Some Dairy Products in Beni-Suef Governorate, Egypt
161
167
EN
Ahmed
Megawer
Milk Hygiene Department, Faculty of Veterinary Medicine, Beni-Suef University, Egypt
salehmazen2016@gmail.com
Gamal
Hassan
Food Safety and Technology Department, Faculty of Veterinary Medicine, Beni-Suef University, Egypt
gamalhassen2000@yahoo.com
Arafa
Meshref
Food Safety and Technology Department, Faculty of Veterinary Medicine, Beni-Suef University, Egypt
foodhyg@yahoo.com
Hala
Elnewery
Bacteriology Department, Animal Health Research Institute, Beni-Suef branch, Beni-Suef, Egypt
dr.hala.elnwary@gmail.com
10.21608/jvmr.2021.148829
<span>The aim of the study was directed to investigate the prevalence of Escherichia coli in raw milk, cream, large and small scale yoghurt, Kareish cheese, and large and small scale ice cream by conventional bacteriological methods as well as detection of the wzy gene (O-antigen polymerase gene) using polymerase chain reaction for further identification of recovered isolates. A total of 200 random samples including 40 raw milk, 40 fresh cream, 20 of each large and small scale yoghurt, 40 Kareish cheese and 20 of each large and small scale ice cream were obtained from different localities in Beni-Suef governorate, Egypt. The present study could provide useful information for the prevalence of E. coli in the examined samples: 75%, 62.5%, 0%, 25%, 80%, 10% and 25% respectively. E. coli isolate were serologically identified as: O26, O111:H4, O121, O125:H21, O169, O126:H7 and O158 in raw milk, O6:H16, O55:H7, O119:H6, O125:H6 and O146:H21 in fresh cream, O55:H7, O125:H21 in small scale yoghurt and O18, O55:H7, O114:H21, O158, O125:H21and O153:H45 in Kareish cheese. Interestingly PCR successfully amplified the wzy gene (O-antigen polymerase gene) in all E. coli isolates which is associated with LPS biosynthesis and bacterial pathogenicity and increases the ability of E. coli to withstand the anti-bacterial defense mechanism. </span>
Dairy products,E. coli,Milk,PCR,serology,wzy gene
https://jvmr.journals.ekb.eg/article_148829.html
https://jvmr.journals.ekb.eg/article_148829_7f7cdca0b7d4b2b0afd141af686ad02c.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
27
2
2020
12
01
Isolation and Molecular Characterization of Marek’s Disease Virus from Layer Chickens in Egypt
168
176
EN
Samar
Ewies
0000-0001-8790-0156
Department of Virology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt
samarewies@yahoo.co.uk
Wesam
Mady
National Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, P.O. Box 264-Dokki, Giza-12618, Egypt
wesammady83@hotmail.com
Enas
Hamad
Animal Health Research Institute, Mansoura
branch, Egypt
enashamah@yahoo.com
Abdelsatar
Arafa
National Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, P.O. Box 264-Dokki, Giza-12618, Egypt
araby85@hotmail.com
Sabry
Tamam
0000-0002-5583-4737
Department of Virology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt
s.mtamam@yahoo.com
Hanafy
Madbouly
0000-0001-9896-5182
Department of Virology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt
hmmadbouly@gmail.com
10.21608/jvmr.2021.59613.1032
Marek’s disease virus is a lymphomatous neuropathic disease of domestic fowl. In Egypt, poultry flocks are suffering from recurrent uprising of MD outbreaks due to increased virulence of the virus. In this study, a total of 10 clinical samples were collected from 6-8 months old vaccinated layer chickens suffering from depression, ataxia and paralysis. These samples were examined for the presence of MDV by PCR using specific primers for ICP4 gene. MDV was detected in 5 out of 10 samples. One out of the 5 positive samples was isolated and partially sequenced for ICP4 gene (F1352-S4). The results proved that it was genetically characterized as very virulent strain of Marek's disease virus type 1 resembling Gallid herpesvirus 2 isolates from Europe, China, Africa and India. There was a characteristic serine residue at the coding region of ICP4 gene encoding the reverse complement protein found only in the Egyptian virus (F1352-S4) and EU-1 virus, IND-KA-02-06, JL/1404 and in Debre-zeit/01/2013 virus that is tracing for the original virus CV1988. The obtained results indicated the importance of continuous monitoring of MDV infection in chicken layer flocks.
Marek’s disease virus,PCR,ICP4 gene,Egypt
https://jvmr.journals.ekb.eg/article_151255.html
https://jvmr.journals.ekb.eg/article_151255_f6389a60b461140874c0a1bcdc43c1fb.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
27
2
2020
12
01
Histopathological Studies on the Curative Role of Mentha longifolia in Trypanosoma evansi Experimentally Infected Rats
177
189
EN
Rasha
S.
Mohamed
Animal Health Department, Desert Research Center, Egypt
rashanono2008@yahoo.com
10.21608/jvmr.2021.61380.1033
<span>Trypanosomiasis is an important protozoan disease of domestic animals and man. One of the important pathogenic trypanosomes in animals is <em>Trypanosoma evansi</em>; the causative agent of Surra that is highly fatal to a number of domesticated mammals such as camels. <em>Mentha longifolia</em> is a plant that has been used as traditional medicine to treat some parasitic and microbial diseases in different countries. This study was conducted to evaluate the antitrypanosomal activity of the leaf ethanolic extract of M. longifolia against experimentally induced <em>T. evansi</em> infection in rats. A total of 16 male albino rats were allocated into four groups of four rats each; uninfected control group (CG), plant extract group (PG), trypanosome infected group (TG), and infected treated group (plant extract + trypanosome) (PTG). Blood and tissue samples were collected from rats on the 35th day after <em>T. evansi</em> infection and treatment. Hematological results obtained from <em>T. evansi</em>-infected rats showed microcytic hypochromic anemia with a significance of (P< 0.05). There was a significant (P< 0.05) increase in the total leukocytic count (TLC) and number of neutrophils, eosinophils, and monocytes in TG) compared to those in CG, PG, and PTG. Histopathological examination revealed necrosis, hydropic degeneration and liver apoptosis, hemorrhage, edema, inflammatory cell infiltration of kidney, demyelination of brain, lymphocytic granuloma of lung. Results of the present study showed that the antitrypanosomal activity of <em>M. longifolia</em> extract was not effective in vivo, despite its high antitrypanosomal activity in vitro. </span>
antitrypanosomal activity,Histopathological examination,Mentha longifolia,Rats,Trypanosoma evansi
https://jvmr.journals.ekb.eg/article_151959.html
https://jvmr.journals.ekb.eg/article_151959_8e331036a325e73a1c96042d2bc7974d.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
27
2
2020
12
01
Gross Morphology and Scanning Electron Microscopic Structure of the Oropharyngeal Cavity of the Domestic Geese (Anser anser domesticus)
190
202
EN
Mohamed
Gomaa
Tawfiek
0000-0002-1254-4529
Anatomy and Embryology Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt
mgtawfiek@vet.bsu.edu.eg
HebatAllah
Hamdy
Mahmoud
Anatomy and Embryology Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt
mgtawfiek@yahoo.com
10.21608/jvmr.2021.61981.1034
<span><span>This study aimed to provide a full morphological description of the oropharyngeal cavity in the domestic geese with gross examination, morphometric analysis and scanning electron microscopy. Eight heads of adult healthy geese were used in this study. The oropharyngeal roof had a large rostral part with five palatine ridges; one median, two paramedian and two lateral longitudinal rows of blunt tubercles bounded laterally by transverse horny lamellae of the beak. The caudal papillary region exhibited choanal and infundibular clefts, surrounded by caudally directed conical papillae. In floor, an elongated tongue had a rounded apex with lingual nail and carried filiform papillae on its lateral edges. Each side of the lingual body carried 9 small conical papillae on the anterior part and 6 giant conical papillae on middle and posterior parts. These conical papillae were distributed among the filiform and hair-like papillae. The Posterior part of lingual body was thickened forming the lingual prominence with a transverse row of caudally directed 8-10 conical papillae forming a papillary crest. Lingual root consisted of a triangular surface surrounded by spinated borders. Caudally, an ovoid laryngeal mound with glottis is located in the pharyngeal floor, with conical papillae on its borders and transverse rows of large-sized pharyngeal papillae arranged linearly as 4-5 papillae on posterior part of the laryngeal mound. Openings of the salivary glands were observed in their corresponding region. In conclusion, the morphological characteristics of the oropharyngeal structures in geese confirmed their adaptation to the feeding habits and type of available food particles. </span></span>
anatomy,Domestic geese,Oropharyngeal cavity,scanning electron microscopy (SEM),Tongue
https://jvmr.journals.ekb.eg/article_152102.html
https://jvmr.journals.ekb.eg/article_152102_190d903277ca937f39739822e8c2e03e.pdf