Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
17
2
2007
03
01
Protein analysis for comparison between Salmonellae isolated from different poultry species
1
9
EN
Seham A.
El-Zeedy
Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo Egypt.
Hussein K.
Eldeen
Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo Egypt.
Jihan M.
Badr
Animal Health Research Institute, Dokki Giza, Egypt.
10.21608/jvmr.2007.77902
A total of 620 egg samples from different species (chickens, ducks and ostriches) and 1615 poultry samples (chickens, ducks, pigeons, quails, turkeys and ostriches) were examined for salmonella infection. 12 salmonella isolates were obtained from the egg samples (1.9%) and 67 isolates from poultry samples (4.1%). Salmonella isolates were serotyped into S. enteritidis (25 isolates), S. typhimurium (17 isolates), S. infantis (12 isolates), S. montivideo (7 isolates), 3 isolates for each of S. rubislaw and S. cerro , 2 isolates for each of S. virginia, S. agona, S. poona, and S. derby and 1 isolate for each of S. sandiago and S. kentucky. The incidence of isolation from different poultry species was discussed in details. Antibiogram of the isolated salmonellae against 10 different antibiotics revealed that norofloxacin, ciprofloxacin, cepheridin and gentamycin gave the highest activity against different salmonella isolates while amoxicillin, tetracycline, and nitrofurantoin showed the highest resistance rate. Pathogenicity of the isolated serovars was tested in chickens. All isolates were found pathogenic with various degree of virulence. SDSPAGE protein analysis for the salmonella isolated form different poultry species revealed 12 protein bands ranged from 22-289 kDa. The differences were insufficient for reliable differentiation between the isolates and accordingly, it could be used beside other molecular techniques in differentiation between the salmonella strains.
protein,analysis,Comparison,Salmonellae,isolated,Poultry
https://jvmr.journals.ekb.eg/article_77902.html
https://jvmr.journals.ekb.eg/article_77902_a58646a12e0bab5f0ababc314d687a3f.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
17
2
2007
03
01
Trial to increase the sensitivity of Brucella antigens treated with Binary ethylene imine as inactivated agent
10
14
EN
Hussein K.
Eldeen
Veterinary Serum and Vaccine Research Institute, Abbassia, Cairo, Egypt.
Salwa S.
Awad
Veterinary Serum and Vaccine Research Institute, Abbassia, Cairo, Egypt.
10.21608/jvmr.2007.77903
kills Brucella cells by causing lysis of the membrane, so the phenol-heat killed brucella antigen may lake specificity as a result of destruction the majority of proteins in the cell wall. Accordingly, attention was directed to produce antigen using binary ethylene imine as an inactivator. The produced antigen showed high specificity in detecting Brucella abortus and Brucella melitensis-infected animals, but sensitivity was not affected in comparison with the standard Rose Bengal antigen. In Enzyme immunotransfer blot (EITB), phenol–heat killed brucella cells showed only 3 bands (37.375, 23.47 and 7.83 kDa) that denotes denaturation for at least 6 bands whereas binary inactivated brucella cells showed similarity with non-treated ones
Increase,sensitivity,Brucella,antigens,Binary,ethylene imine,Agent
https://jvmr.journals.ekb.eg/article_77903.html
https://jvmr.journals.ekb.eg/article_77903_8b68acb99bfe9fb6f0a1a6cbfdfca389.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
17
2
2007
03
01
Serodiagnostic studies on bovine leptospirosis in Beni-Suef Governorate
15
20
EN
W. H.
Hassan
Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine,
Beni-Suef University, Beni-Suef, Egypt
10.21608/jvmr.2007.77904
The present study was carried out in dairy farms experiencing low reproductive efficiency. Blood samples were collected from 84 cattle and 16 buffaloes suffered from infertility problems for detection and titration of leptospiral antibodies using the microscopic agglutination test (MAT). Eleven standardized leptospira serovars were used as living antigens for this purpose. Sixteen (19.05%) and 2 (12.5%) samples were found positive for L. interrogans serovar Icterohaemorrhagiae for cattle and buffaloes respectively, with titers ≥1:200. Antibodies against L. interrogans serovar Pomona were detected in 8 (9.52%) and 2 (12.5%) in cattle and buffaloes respectively with titers ≥1:400. Two cattle (2.38%) and two buffalo (12.5%) samples were positive for L. interrogans serovar Djasiman. On the other hand, two cattle samples were positive for both L. interrogans serovar Icterohaemorrhagiae and L. interrogans serovar Pomona. Second serum samples were rechecked for seroconversion from each positively reacted animal with 3-4 weeks interval.
Serodiagnostic,studies,leptospirosis,bovine,Beni-Suef
https://jvmr.journals.ekb.eg/article_77904.html
https://jvmr.journals.ekb.eg/article_77904_9f409f7e2539442e9ceef35a232df9a0.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
17
2
2007
03
01
Polymerase chain reaction for differentiation of Pasteurella multocida isolates from turkeys in comparison to strains incorporating in fowl cholera vaccine
21
24
EN
Nahed I.M.M.
Khamis
Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt.
Zeinab M.
Souror
Central Laboratory for evaluation of Veterinary Biologics, Abbasia, Cairo, Egypt.
Hanan N.
Ibrahim
Central Laboratory for evaluation of Veterinary Biologics, Abbasia, Cairo, Egypt.
S. M.
Aboul Saoud
Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt.
2
10.21608/jvmr.2007.77913
In the present study, polymerase chain reaction (PCR) using random primer (E-20) was used to characterize and identify strains included in this study. Strains included 4 vaccinal reference strains of Pasteurella multocida, CU strain and 4 field isolates of Pasteurella multocida isolated from diseased turkeys which were identified biochemically and serologically as A:1, A:3, A3x4 and D:11. The obtained results revealed that all strains were reacted positively and in different manner with the E20 primer except the 2 field isolates. The results of these reactions demonstrated in terms of bands of different molecular weight specific to each strain. This can be used as a base for characterization and differentiation of strains involved in the present study as the 2 field strains A:1 and A:3 react with primer. Mouse protection test was performed by vaccination of mice with local fowl cholera oil adjuvant vaccine then challenge with virulent field strains A:1, A:3, D:12 and untypable isolates. Results revealed that the local fowl cholera adjuvant vaccine could protect mice against virulent challenge with A:1, A:3 and D:12 field strains but it could not be protect mice against untypable isolates
Polymerase,chain,reaction,Pasteurella,multocida,isolates,turkeys,Strains,incorporating,vaccine,fowl cholera
https://jvmr.journals.ekb.eg/article_77913.html
https://jvmr.journals.ekb.eg/article_77913_016073371edf24afa31b8888676b80e2.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
17
2
2007
03
01
Preparation and evaluation of kits for detection of antibodies of Pasteurella multocida
25
27
EN
Zeinab M.
Souror
Central Laboratory for Quality Control of Veterinary Biologics Abbassia, Cairo, Egypt
A. A.
Badawi
Central Laboratory for Quality Control of Veterinary Biologics Abbassia, Cairo, Egypt
Hanan M.
Ibarahim
Central Laboratory for Quality Control of Veterinary Biologics Abbassia, Cairo, Egypt
10.21608/jvmr.2007.77908
Polyclonal hyperimmune serum against Pasteurella multocida type A:5, A:8 and A:9 was prepared in boskat rabbits. The indirect haemagglutination test (IHT) showed that such serum had an antibody titer of 1114. The immunoglobulins in the prepared antiserum were precipitated using saturated ammonium sulphate solution. Its concentration was adjusted to be 18mg/ml in normal saline then it was conjugated with horse radish peroxidase and evaluated through the application of double sandwich ELISA. It was successful to detect Pasteurella multocida antibodies in positive serum samples with strong positive reactions up to a dilution of 1:100 of<br />the prepared conjugate.In the present study, polymerase chain reaction (PCR) using random primer (E-20) was used to characterize and identify strains included in this study. Strains included 4 vaccinal reference strains of Pasteurella multocida, CU strain and 4 field isolates of Pasteurella multocida isolated from diseased turkeys which were identified biochemically and serologically as A:1, A:3, A3x4 and D:11. The obtained results revealed that all strains were reacted positively and in different manner with the E20 primer except the 2 field isolates. The results of these reactions demonstrated in terms of bands of different molecular weight specific to each strain. This can be used as a base for characterization and differentiation of strains involved in the present study as the 2 field strains A:1 and A:3 react with primer. Mouse protection test was performed by vaccination of mice with local fowl cholera oil adjuvant vaccine then challenge with virulent field strains A:1, A:3, D:12 and untypable isolates. Results revealed that the local fowl cholera adjuvant vaccine could protect mice against virulent challenge with A:1, A:3 and D:12 field strains but it could not be protect mice against untypable isolates
Preparation,Evaluation,kits,antibodies,Pasteurella,multocida
https://jvmr.journals.ekb.eg/article_77908.html
https://jvmr.journals.ekb.eg/article_77908_7151038bf26ce761ee138e2dbf3d1a07.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
17
2
2007
03
01
Bovine Parapoxvirus: Isolation and pathogenicity studies
28
34
EN
A. S.
Abdel-Moneim
Department of Virology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt
S. M.
Tamam
Department of Virology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt
10.21608/jvmr.2007.77909
A disease characterized by papules, nodules, vesicles, pustules and ulcers on teats and udder as well as drastic drop in milk production was seen among a cattle farm in Fayoum Governorate, Egypt. A virus was isolated by inoculation of vesicle and scrap homogenate pool from infected cattle into the chorioallantoic membrane of specific pathogen free embryonated chicken eggs. The virus was identified by presence of pock lesions, intracytoplasmic inclusion bodies on the chorioallantoic membrane, polymerase chain reaction and immunohistochemistry of the inoculated membrane. A novel pathogenicity model was developed via ear pinna inoculation of Swiss mice. The virus produced vesicular and ulcerative lesions at the site of inoculation in inoculated mice. The virus identity was confirmed by the presence of intracytoplasmic viral antigens by immunohistochemistry
bovine,Parapoxvirus,isolation,pathogenicity,studies
https://jvmr.journals.ekb.eg/article_77909.html
https://jvmr.journals.ekb.eg/article_77909_ec1d3fb3bd192585d3214c84baf9a378.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
17
2
2007
03
01
Clinical, haematological and some biochemical variations hypophosphataemia in buffaloes before and after treatment at Assiut Government
35
41
EN
M. E
Radwan
Animal Health Research Institute., Assiut Regional Laboratory.
H. Z.
Rateb
Department of Animal Medicine, Faculty of Veterinary Medicine, Assiut University
10.21608/jvmr.2007.77910
A total number of 28 buffaloes aged between 5-7years, body weight ranged between 400-500 kg, and belonged to private farms at Assiut Governorate constituted the materials of this study. Twenty of them showed the classical signs of hypophosphataemia while the other eight buffaloes were proved to be healthy by both clinical and laboratory investigations, used as control group. Biochemical analysis of blood sera showed a highly significant hypophosphataemia, hypocalcaemia and hypomagnesaemia in diseased buffaloes either pre or post treatment when compared with the healthy control ones. Meanwhile, the examination of blood showed marked decreases in erythrocytic count, haemoglobin concentration and packed cell volume in diseased animals. All parameters improved in affected buffaloes after 10 days of treatment. The chemical analysis of agronomical samples of soil and drinking water were done. The statistical analyses between the studied parameters were carried out in buffaloes before and after treatment.
Clinical,haematological,biochemical,Variations,hypophosphataemia,buffaloes,Treatment,Assiut
https://jvmr.journals.ekb.eg/article_77910.html
https://jvmr.journals.ekb.eg/article_77910_b51d14b95b90f473bea9d880dc561496.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
17
2
2007
03
01
Ultrasonography of normal, cystic and dysplastic kidney in cattle
42
49
EN
M. M.
Seif
Surgery, anesthesiology and radiology department, Faculty Veterinary Medicine, Beni-Suef University,
Beni-Suef, Egypt
H. A.
Bakr
Internal Medicine department, Faculty Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt
10.21608/jvmr.2007.77911
Thirty four apparently healthy cattle (9 males and 25 females) of mixed breed (Balady X Friesen) were selected for ultrasonographic investigations in this study. Ultrasonographic measurements of vertical and horizontal diameters of kidney, the diameters of the renal parenchyma and the diameter of renal sinus were determined in the middle of right and left kidneys. Twenty nine cattle (9 males and 20 females), had normal ultrasonographic appearance of both right and left kidney while the other five cows had some pathological affections including cystic kidney and renal dysplasia in their left kidneys . The vertical diameter of the right kidney was (4.84±1.18 cm) , the horizontal diameter (9.16 ± 1.35 cm) , and the vertical diameter of the renal sinus was ( 3.54 ± 1.02 cm ) . The thickness of the renal cortex and medulla ( renal parenchyma) was (2.16 ± 0.46 cm ). On the other hand ,the vertical diameter of the left kidney was (5.89 ±1.13 cm), and the vertical diameter of the renal sinus was ( 3.83 ± 1.12 cm ). The thickness of the renal cortex and medulla (renal parenchyma) was (2.46 ± 0.35 cm ). It was concluded that the ultrasonographic values determined in this study can be used as references for the diagnosis of morphologic changes in the kidney of domestic dairy cattle
Ultrasonography,normal,cystic,dysplastic,kidney,cattle
https://jvmr.journals.ekb.eg/article_77911.html
https://jvmr.journals.ekb.eg/article_77911_7d9553a7fe0243a24d4158e6def2358a.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
17
2
2007
03
01
Gastric neobladder: an experimental study in dog
50
59
EN
M. M
Seif
Department of Surgery, Anaethesiology and Radiology, Faculty of Veterinary Medicine
Beni-Suef University
M. S.
Aimen
Department of Surgery, Anaethesiology and Radiology, Faculty of Veterinary Medicine
Beni-Suef University
H. H.
Kamel
Department of Clinical Pathology, Faculty of Veterinary Medicine Beni-Suef University
10.21608/jvmr.2007.77912
The urinary bladder of 15 clinically normal dogs was excised and the ureters were<br />implanted into an isolated, vagotomized gastric segment derived from the fundic region of the stomach. The gastric segment was closed to form a neobladder. Continence was maintained with a "nipple valve" created at the tubularized end of isolated segment of stomach. Clinical, radiological, ultrasonographical, urine and blood analysis and histopathological examination were carried out for assessment of the technique. Eleven cases showed an apparently normal bladder function. Two cases suffered from renal hydronephrosis and other two suffered from incontinence. It was concluded that gastric neobladder urinary diversion is satisfactory for clinical use in dogs.
Gastric,neobladder,experimental,study,Dog
https://jvmr.journals.ekb.eg/article_77912.html
https://jvmr.journals.ekb.eg/article_77912_147b173a42467965240e95a7cb3a5cb3.pdf