Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
20
1
2010
03
01
Some laboratory studies on freshwater snails and the effect of some plants on their biological control in Beni-Suef governorate
1
5
EN
M. A.
Abdel-Gawad
Animal Health Researches Institute, Beni-Suef Laboratory
A. M.
Khateeb
Animal Health Researches Institute, Beni-Suef Laboratory
10.21608/jvmr.2020.77573
Freshwater snails collected from ditch like ponds near the River Nile in Beni-Suef Governorate during three climatically differed periods. Lymnaea cailliaudi, Biomphalaria alexandarina, Bulinus truncatus, Lanistes carinatus, Cleopatra bulimoids,Physa acuta, Viviparus viviparus, Melania tuberclata and Bivalves species were included. The total number of collected snails in December 2008, March 2009 and July 2009 were 1321, 1370 and 1211 respectively, with larger number in March than in July or December due to optimal climatic conditions in Marsh for most of collected snails. The recorded survival longevity in the laboratory was more than 90 days for Lymnaea cailliaudi, two months for Lanistes carinatus, while in Melania tuberclata, was about three weeks but only two to three days for Bivalves. Cercariae shedding observed from Lymnaea, Lanistes and Biomphalaria species beside stylet cecariae from Lymnaea and Lanistes snails, also rediae and sporocysts were found in crushed snails in all periods of collections with little difference between these periods, about 14% of crushed Lymnaea were containing rediae. Aquatic insects and their larvae were found especially water bug Sphaerodema sp. found predating snails or their eggs, also the mature insect still alive in the breeding container all over the period of the experiment more than three months. Culex larvae attacked egg masses of the snails, estacosa predated the snails while Telapia nilotica fishes showed low affinity to predate such snails. The oleo-gum resin Myrrh (Commiphora molmol) in the concentration of 0. 5gm/1 litre water killed 90 % of the exposed snails after 72 hours.
freshwater snails,plants,control
https://jvmr.journals.ekb.eg/article_77573.html
https://jvmr.journals.ekb.eg/article_77573_cbdbee6afc5af87da1209e87fb66bbac.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
20
1
2010
03
01
Evaluation of the protective capacity of Toxocara canis embryonated egg antigen in rabbits
6
12
EN
S. M.
Aboel Hadid
Department of Parasitology Faculty of Veterinary Medicine, Beni-Suef University
10.21608/jvmr.2020.77574
Toxocariasis is a disease of unspecific clinical manifestations in human beings and some animals forming a condition known as visceral larva migerans. Effective Toxocara canis (T. canis) control should destroy the dormant hypobiotic larvae in the tissues of the reservoir hosts. Embyonated egg antigen is intended to be evaluated in rabbits where15 New Zealand rabbits were divided into 3 groups; immunized group that injected with three doses of T. canis egg antigen (First dose with complete Freund's and two successive doses with incomplete Freund’s adjuvant); control infected group and adjuvant control group. At 30th day from the beginning of the experiment, all groups were infected with 10,000 T. canis embryonated egg. Blood samples were collected periodically for measuring antibodies titer by ELISA. Postmortem and histopathological investigations were done. The efficacy of egg antigen protective immunity was evaluated by; the positive antibody titer, retention of larvae in the liver, hepatic eosinophilic granuloma formation, number of the milky spots on the liver surface and lung resistance to the infection. These parameters were detected obviously in the immunized group than the non immunized ones. Consequently, production of this product is recommended where its preparation is simple, easy, fast and economic and may be used as role of vaccine program against the parasite infection in the<br />susceptible hosts.
Evaluation,protective,capacity,Toxocara,canis,egg,Rabbits
https://jvmr.journals.ekb.eg/article_77574.html
https://jvmr.journals.ekb.eg/article_77574_8a642db405bda949cbf5d02dab599e5d.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
20
1
2010
03
01
Effect of Eimeria stiedae infestation on the immune response of rabbit vaccinated with oil adjuvant polyvalent rabbit Pasteurellosis
13
18
EN
Elham.
A. Youssef
Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt
N. B.
Eskander
eterinary Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt
Lilian.
F. S. Melika
Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt
Amina
A. El-Bayoumy
Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt
M. S.
El-Nabarawy
Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt.
10.21608/jvmr.2020.77575
In an attempt to evaluate the possible role of Eimeria stiedae infection on rabbit vaccinated with haemorrhagic septicaemia oil adjuvant vaccine, a total of 60 New-Zealand rabbits were divided into 6 groups (A- F). The first four groups subdivided into two subgroups. The subgroups (A1, A2) vaccinated and infected at time of 1st dose of vaccine, subgroup (B1, B2) vaccinated and infected at 2 weeks post 1st vaccination, subgroup (C1, C2) which vaccinated and infected at the time of 2nd dose of vaccination, finally subgroup (D1, D2) vaccinated and infected at 2 weeks post<br />2nd dose of vaccine. Group E vaccinated only but the group F left as non vaccinated non infected (control). The results revealed that E. stiedae infection at the time or after 2 weeks from first or second dose of vaccination (A1, B1, C1 and D1) and treated with semduramycine 150 showed slight decrease of the antibody titer in contrast the untreated group (A2, B2, C2 and D2) showed sudden decrease of P. multocida antibody titer measured by indirect haemagglutination and ELISA test. Vaccinated group (E) was the superior one showing the highest antibody titer. The challenge test of all rabbit groups with virulent P. multocida revealed a protective percent of 83.4%, 50%, 100% and 0 % in treated, untreated, vaccinated and control group respectively, but subgroups C2, D2 the protective value was 33.4% this due to challenge concurrency post or at the time of infection. These findings reflect the important to avoid coccidial infection following vaccination programs to obtain better immune response to haemorrhagic septicaemia oil adjuvant pasteurellosis vaccine<br />and high level of protection.
Effect,Eimeria,stiedae,Infestation,Immune,Rabbit,vaccinated,oil,Pasteurellosis
https://jvmr.journals.ekb.eg/article_77575.html
https://jvmr.journals.ekb.eg/article_77575_4d0bf2b76fde52f1dcd6f01e5c7aa375.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
20
1
2010
03
01
Incidence of hoof affections in equine
19
29
EN
M. M.
Seif
Department of Surgery, anesthesiology and radiology, Faculty of Veterinary Medicine, Beni-Suef University
A. M.
Sedeek
Department of Surgery, anesthesiology and radiology, Faculty of Veterinary Medicine, Beni-Suef University
M. Z.
Fathy
Department of Surgery, anesthesiology and radiology, Faculty of Veterinary Medicine, Beni-Suef University
G. A.
Ragab
Department of Surgery, anesthesiology and radiology, Faculty of Veterinary Medicine, Beni-Suef
University
10.21608/jvmr.2020.77576
This study was conducted on 1178 lamed animals of equine species. Cases were classified according to the species, affected part of the foot, and nature of the affection. Hoof affections represented in about 722 animals with 61.3 % of total lame animals. The percentages of hoof affections were higher in forefeet than hind feet. The present study revealed that sandy cracks, toe- in and long toe were the predominant hoof wall affections. The corn was the highest sole affections followed by white line disease and the lowest percentages were flat foot and bruised sole. The highest affection percentage in frog was thrush followed by picked up nail and the lowest was in under run heel.
Incidence,hoof,affections,equine
https://jvmr.journals.ekb.eg/article_77576.html
https://jvmr.journals.ekb.eg/article_77576_39d4a5a2639167b8eb1f507da589fb68.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
20
1
2010
03
01
Radiologic and ultrasonographic studies of kidneys in goat
30
37
EN
G. A.
Ragab
Department of Surgery, anesthesiology and radiology, Faculty of Veterinary Medicine, Beni-Suef
University
M. M.
Seif
Department of Surgery, anesthesiology and radiology, Faculty of Veterinary Medicine, Beni-Suef University
U. A.
Hagag
epartment of Surgery, anesthesiology and radiology, Faculty of Veterinary Medicine, Beni-Suef University
10.21608/jvmr.2020.77577
A study was performed on fifty normal goats for radiographic (only 20 animals), and sonographic (the all 50 animals) investigations. Radiographic results revealed that, both kidneys had bean shaped and smooth contour. The right kidney position was constant due to its relation with the liver, while, the left kidney was mobile and its position varied according to the degree of fullness of the rumen due to its long mesentery. The sonographic results revealed the position, shape, dimensions and echogenicity of kidneys, by using 3.5/5.0 MHz curved transducer and 6.0/8.0 MHz linear transducer. The renal capsule was represented by a very fine echogenic lines around the kidney that couldn’t be distinguished from the peri-renal fat. The cortex was more echogenic than the medulla and had uniform finely granular hypoehoic pattern. The corticomedullary junction was slightly more echogenic than the cortex due to the presence of the arcuate arteries. The medulla was observed as hypoechoic area interrupted by bright echogenic lines representing the recesses and interlobular arteries that gave the appearance of typical medullary pyramids. The dimensions of the right kidney were 5.32 ± 0.12, 2.79 ± 0.07, 0.64 ± 0.01, 1.52 ± 0.03, 2.74 ± 0.09, 1.27 ± 0.06, 1.25 ± 0.05, and 1.72 ± 0.09; and the dimensions of the left kidney were 5.34 ± 0.12, 2.80 ± 0.07, 0.65 ± 0.01, 1.55 ± 0.03, 2.76 ± 0.09, 1.26 ± 0.06, 1.28 ± 0.05, and 1.73 ± 0.1, for the length, width, cortical thickness, medullary thickness, depth, diameter of renal sinus, diameter of renal parenchyma, and circumference of renal pyramids respectively.
Radiologic,ultrasonographic,kidneys,Goat,studies
https://jvmr.journals.ekb.eg/article_77577.html
https://jvmr.journals.ekb.eg/article_77577_5086b4d1505eb22b583f30434889692f.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
20
1
2010
03
01
Impairment of female Oreochromis niloticus fecundity exposed to Butachlor herbicide
38
43
EN
Manal
A. A. Essa
Department of Fish, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef,62511, Egypt,
M. S.
Marzouk
Department of Fish Diseases and Management, Faculty of Veterinary Medicine, Cairo University,
Cairo, Egypt
Nashwa
S. Elias
Department of Fish Diseases, Animal Health Research Institute, Giza, Egypt
Maysa
H. Shaker
Department of Pathology, Animal Health Research Institute, Giza, Egypt.
Ghada
M. A. Mohamed
Department of Pathology, Animal Health Research Institute, Giza, Egypt.
10.21608/jvmr.2020.77578
Female Oreochromis niloticus were exposed to 1/10 LC50 (0.21ppm) of butachlor herbicide for 6 weeks. Weekly specimens were taken for fecundity estimation. Also hormonal and enzymatic levels were determined in addition histopathological alterations in ovaries and liver were detected. Butachlor exerted drastic effects on absolute and relative fecundity. Sex hormones (testosterone "T" and estradiol "E2") dropped significantly. The high significantly decline in Total Ripen Egg Number was assisted by the coagulative necrosis and oocytic atrasia in ovaries. In addition, thrombus formation and hepatoadenocarcinoma were pronounced in the liver and resulted in the significant drop in ALT and total protein levels. So, it is recommended to apply the biological control of pests in substitution to herbicids in rice fields.
Oreochromis niloticus,Impairment,Female,fecundity,Butachlor,herbicide
https://jvmr.journals.ekb.eg/article_77578.html
https://jvmr.journals.ekb.eg/article_77578_d0c9257a6765032f2e03cce22dc016bd.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
20
1
2010
03
01
Light and electron microscopic studies of the prostate gland of adult one humped camel (camelus dromedarius)
44
51
EN
S. M.
Soliman
Department of Cytology and Histology, Faculty of Veterinary Medicine, Beni-Suef University
K. M.
Mazher
Department of Cytology and Histology, Faculty of Veterinary Medicine, Beni-Suef University
A. H.
Abdelrazek
Department of Cytology and Histology, Faculty of Veterinary Medicine, Beni-Suef University
10.21608/jvmr.2020.77579
The present study aimed to clarify the light and electron microscopic structure of the prostate gland of mature (one-humped camels) during different seasons of the year. Glands of seventy-two mature healthy animals (5-7 years old) were collected from the Cairo slaughter house during one year, (6 samples each month) and prepared to be studied microscopically by the light and electron microscope. The prostate gland was found to be consisted of an external dorsal part dorsal to the neck of the bladder and an internal part situated in the submucosa of the prostatic urethra. During active season (winter and spring), the corpus prostate was enveloped by a thick fibromuscular capsule which sent septa, to divide the gland into lobules. The parenchyma formed of compound tubuloalveolar adenomeres. The alveoli and tubules were lined by high columnar cells and few basal ones. The acini appeared at different stages of secretory activity (synthesis, storage, secretion and exhaustion). Ultrastructurally, the acinar cells contained well developed rough endoplasmic reticulum (rER), numerous mitochondria and a variable number of secretory granules. The duct system began as central collecting sinuses lined by simple columnar secretory epithelium. The pars interna occurred in the submucosa of the prostatic urethra enveloped by a thick fibro-muscular band. The branched tubuloalveolar parenchyma contained adenomeres lined with simple cuboidal epithelium. During the inactive season (summer and autumn), the stroma showed a marked proliferation of the fibromuscular tissue on the expense of the parenchymatous tissue. The adenomeres became very small or even rudimentary with narrow lumina devoid of secretory materials. Marked reduction in the cytoplasmic organelles with a total absence of the secretory granules was also pronounced.
Light,electron,microscopic,studies,prostate,gland,humped camel,Camelus dromedarius
https://jvmr.journals.ekb.eg/article_77579.html
https://jvmr.journals.ekb.eg/article_77579_276202e1662de9eeef71c44475d50549.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
20
1
2010
03
01
Light and electron microscopic structure of goat's retina
52
62
EN
S. M.
Soliman
Department of Cytology and Histology
Z. A. A.
Adam
Department of Cytology and Histology,Faculty of Veterinary Medicine, Beni-Suef University
U. K. M.
Abd allah
Department of Cytology and Histology,Faculty of Veterinary Medicine, Beni-Suef University
10.21608/jvmr.2020.77580
The present study was conducting aiming to throw the light on the retinal structure on the level of both light and electron microscope. Eyeballs of 35 adult clinically healthy goats of both sexes were collected from Beni- Suef abattoir. The eyeballs were clinically examined before they were dissected and fixed in 10% buffed neutral formalin and in Bouin’s solution for 24 hours. The specimens were then processed for light and transmission electron microscope. The retina (pars optica retinae) of the goats extends rostrally to cover the ciliary body as pars ciliaris retinae and the iris as the pars iridis retinae. Pars optica retinae and pars iridis retinae form the light non sensitive parts of the retina, while the sensitive part except at the transition zone; the ora serrata and the optic disc, appeared to be formed of ten layers, named from outward to inward as, retinal pigmented epithelium, rods and cones layer (photoreceptor cell layer), external limiting membrane, outer nuclear layer (cell bodies and nuclei of the photoreceptor cells), outer plexiform layer, inner nuclear layer (contained the horizontal, bipolar, Muller and amacrine cells), inner plexiform layer, ganglionic cell layer, nerve fiber cell layer (unmyelinated nerve fibers) and<br />internal limiting membrane.
Light,electron,microscopic,structure,goat's retina
https://jvmr.journals.ekb.eg/article_77580.html
https://jvmr.journals.ekb.eg/article_77580_b4430b1cc44a9ea4cdc3f112668a2e7c.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
20
1
2010
03
01
Bovine Ephemeral Fever: Pathological and Immunohistochemical Studies
63
68
EN
K. A.
El-Nesr
Department of Pathology, Faculty of Veterinary Medicine, Beni Suef University, Beni-Suef 62511,
Egypt
E. A.
Mahdy
Department of Pathology, Faculty of Veterinary Medicine, Beni Suef University, Beni-Suef 62511, Egypt
M. B.
El-Begaway
Department of Pathology, Faculty of Veterinary Medicine, Beni Suef University, Beni-Suef 62511, Egypt
10.21608/jvmr.2020.77581
A natural outbreak of Bovine Ephemeral Fever in Egypt during the summer of 2006 had been observed. In Beni Suef province, out of 70 cattle naturally infected with bovine ephemeral fever virus, three fattening calves suffered from subcutaneous emphysema died and were subjected to post-mortem examination. The findings revealed severe subcutaneous emphysema, interstitial and pulmonary emphysema. The serous membranes were thick, opaque and emphysematous. Microscopically, interstitial and pulmonary emphysema was prominent in most lobes of the examined lungs accompanied with pulmonary oedema and focal leucocytic aggregations in some areas. Angiopathy was demonstrated in all cases. The bronchial and mediastinal lymph nodes showed congestion and hemorrhages. Immunohistochemically, specific reaction for Bovine Ephemeral Fever virus was demonstrated in the lung and lymph nodes of the three cases; the pathogenesis of the disease was discussed.
bovine,Ephemeral,pathological,Immunohistochemical,studies
https://jvmr.journals.ekb.eg/article_77581.html
https://jvmr.journals.ekb.eg/article_77581_360cdbb0ad0e68f805b5965843548250.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
20
1
2010
03
01
Pathological studies on fallopian tube in relation to uterine lesions and ovarian abnormalities
69
75
EN
E. A.
Mahdy
Department of Pathology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt
10.21608/jvmr.2020.77582
A total number of 50 genital tracts were collected from cows slaughtered at “Belefia” abattoir in “Beni-Suef” governorate, Egypt. The genitalia were inspected grossly and the ovarian activity was noticed. Tissue specimens were taken from the tips of uterine horns, fallopian tubes, ovaries of both sides. The fallopian tubes were cut and sampled at three levels namely, infundibulum, ampulla, isthmus. All tissue specimens were fixed in 10% formalin solution, embedded in paraffin wax, sectioned at 5 μm and stained with hematoxylin and eosin stain (Bancroft and Stevens 1996) and examined microscopically. The pathological changes in the fallopian tube in relation to lesions in the uterus and ovarian activity were investigated. The uterine pathological lesions were endometritis (12%), adenomyosis (12%), and cystic glandular hyperplasis (8%). Inflammation of the fallopian tube salpingitis, was graduated as mild degree(18%), moderate degree(2%), and severe degree (2%). The intraepithelial microcysts of the uterine tube represented 8% of the examined cases.
pathological,studies,fallopian,tube,Relation,uterine,lesions,Ovarian,abnormalities
https://jvmr.journals.ekb.eg/article_77582.html
https://jvmr.journals.ekb.eg/article_77582_f6980a8aaea0673e729639dc4b5409dd.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
20
1
2010
03
01
Haemato-chemical, histopathological and immunological studies on chicks infected with low pathogenic avian influenza virus (H5N2)
76
84
EN
Hala
M. E. El-Makaki
Veterinary Serum and Vaccine Research Institute
O. G. A.
Salman
Veterinary Serum and Vaccine Research Institute,Abbasia, Cairo, Egypt
Lamiaa
M. Omar
Veterinary Serum and Vaccine Research Institute,Abbasia, Cairo, Egypt
N.A.
Sherif
Veterinary Serum and Vaccine Research Institute,Abbasia, Cairo, Egypt
Hoda .
Tawfik
Veterinary Serum and Vaccine Research Institute,Abbasia, Cairo, Egypt
10.21608/jvmr.2020.77583
The haematochemical, histopathological and immunological studies were carried out on chicks experimentally infected with the low pathogenic avian influenza virus (LPAIV) (A/Turkey/CA/209092/02) H5N2. Eighty SPF one day old chicks were serologically negative for specific antibodies against avian influenza virus. The birds were devided into 2 groups, birds in the 1st group were inoculated with the virus via the intraocular and intranasal routs, while the other group was kept as non-infected control. Five birds were sacrificed from both groups at 5, 7, 10, 15, and 21 days post inoculation. Sera and heparinized blood as well as tissue specimens from lung, liver, spleen, trachea, small intestine and bursa of Fabricius were collected. Estimation of haemagglutination inhibition antibodies response against AI, liver and kidney function tests, rate of proliferation of T-lymphocyte were conducted. The experimentally infected birds showed general signs of illness with 80% morbidity and 6 % mortality. There was an increase in aniline aminotransferase (ALT) and asparate aminotransferase (AST) enzymes which reflected liver damage. High urea and creatinine values were also detected in sera of infected birds which proved kidney dysfunction. There was no significant proliferation of T-lymphocyte among examined groups. Very low haemagglutinating inhibiting (HI) antibodies was detected in infected birds. Histopathological examination displayed conspicuous depletion and necrosis of the lymphocytic aggregation in the organs of the haemobiotic system (Bursa of Fabricius, spleen and thymus). Such finding may decipher the low sero-conversion as well as the unproliferation of T-lymphocyte. The necrobiotic changes in liver and kidney sections in addition to congestion and edema elucidate the increased parameters in their functions. Also, the epithelial hyperplasia of the tracheal mucosa and the sloughing in the lining mucosal epithelium are indicative for the epithelio-tropism of the AI virus.
Haemato-chemical,Histopathological,immunological,studies,chicks,infected,low pathogenic,avian,influenza,virus,(H5N2)
https://jvmr.journals.ekb.eg/article_77583.html
https://jvmr.journals.ekb.eg/article_77583_a256437973cbc16517821f36be4e7012.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
20
1
2010
03
01
Some serum biochemical and pathological changes in squabs of domestic pigeons (Columba Livia) infected with Trichomonas
85
98
EN
H. E.
Abbas
Department of Biochemistry, Animal Health Research Institute, Dokki, Giza
Hanan A.
Tag El-Din
Department of Biochemistry, Animal Health Research Institute, Dokki, Giza
E. K.
Soliman
Department of Biochemistry, Animal Health Research Institute, Dokki, Giza
Leila A.
Tantawy
Department of Biochemistry, Animal Health Research Institute, Dokki, Giza
10.21608/jvmr.2020.77584
The present study was carried out to represent a field problem in squabs of domestic pigeons (columba livia) at Ismailia Province. Squabs were grossly examined and showed typical lesions including yellowish caseous, fibronecrotic patches in mouth due to infection with T. gallinae. Forty squabs were collected and tested individually for the presence of Trichomonas gallinae (T. gallinae). Squabs were divided into equal four groups, the 1st was un-infected control group, the 2nd was T. gallinae infected untreated group, the 3rd and the 4th groups were T. gallinae infected and treated with metronidazole. The obtained results showed that the mortality (%) were 0, 50, 20 and 30 % in 1st, 2nd, 3rd and 4th group, respectively. Body weight was significantly reduced in all groups, although the drugs improved the weight reduction as compared to pre-treatment. Organs' weights were significantly increased after treatment as compared with the control group. Serum biochemical analysis revealed significant elevation in total protein, globulins; β- globulin and γ- globulin but albumin , α-globulin levels and A/G ratio were significantly reduced in infected squabs and increased in treated groups. Serum urea, creatinine and uric acid levels were increased, while, Serum glucose , cholesterol Na, K, Ca, P, Mg and serum iron as well as plasma ChE activity were decreased in both treated and infected groups. Serum AST, ALT, LD, γ -GGT, CK, AP activities were significantly increased in infected groups, Destructive changes in buccal cavity, hyperemia in blood vessels, necrotic changes in the liver with leucocytic infiltration and demylination of brain with preivascular oedema were observed.
serum,biochemical,pathological,changes,squabs,domestic,Pigeons,Columba Livia,infected,Trichomonas
https://jvmr.journals.ekb.eg/article_77584.html
https://jvmr.journals.ekb.eg/article_77584_408dcf3a63f73cb16e0809563227d0c8.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
20
1
2010
03
01
Effect of some antioxidants on the reproductive performance in rats
99
105
EN
S. S.
Ibrahim
Department of Physiology, Faculty of Veterinary Medicine, Beni-Suef University
A.
Aboul-Ela
Department of Physiology, Faculty of Veterinary Medicine, Beni-Suef University
E. A.
Mabrouk
epartment of Physiology, Faculty of Veterinary Medicine, Beni-Suef University
A. A.
Mohammed
Department of Physiology, Faculty of Veterinary Medicine, Beni-Suef University
10.21608/jvmr.2020.77585
The present study was designed to determine the effect of the antioxidants; taurine, ascorbic acid '' AA '' and beta-mercaptoethanol '' β-ME '' separately or in combination, on reproductive performance of adult male rats suffering from cadmium chloride "CdCl2" - induced oxidative stress. A total of 180 mature male Albino rats were equally divided into 9 groups; the rats of the 1st were administered distilled water while those of the 2nd group were administered 1/100 LD50 of CdCl2. Rats of the remaining groups were administered 1/100 LD50 of CdCl2 followed 3 days later by 1 / 50 LD50 of either taurine, AA and β-ME or a combination of AA and taurine, AA or β-ME, taurine and β-ME as well as AA, taurine and β-ME, respectively for 2 successive months. The results revealed that application of CdCl2 for 8 successive weeks decreased pituitary and serum levels of gonadotropins (gametogenic hormone”FSH” and interstitial cell stimulating hormone “ICSH”) as well as serum testosterone “T” level, altered semen quality, and decreased serum level of total antioxidant capacity with increased serum malondialdhyde "MDA" level. On the other side, application of different antioxidants to CdCl2 –induced oxidative stress increased pituitary and serum levels of gonadotropins and serum level of T as well as improved semen quality, increased serum level of total antioxidant capacity and decreased serum level of MDA. In addition, the best improvement in male reproductive performance was achieved after administration of AA and taurine separately or in combination while the least improvement was obtained when β-ME was applied alone.
Effect,antioxidants,reproductive,performance,Rats
https://jvmr.journals.ekb.eg/article_77585.html
https://jvmr.journals.ekb.eg/article_77585_c0132367bccecf24e8c7a8d07481cb84.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
20
1
2010
03
01
Clinicopathological and immunological studies on Toxoid vaccine as a successful alternative in controlling clostridial infection in broilers
106
115
EN
Nahed
Saleh
Department of Clinical Pathology Faculty of Veterinary Medicine, Menufiya University, El-Sadat Branch,
Egypt.
Rasha
Nabil
Department of Clinical Pathology,Faculty of Veterinary Medicine, Menufiya University, El-Sadat Branch, Egypt.
S.
Fathalla
Department of Clinical Pathology,Faculty of Veterinary Medicine, Menufiya University, El-Sadat Branch, Egypt.
A.
Mosaad
Department of Clinical Pathology,Faculty of Veterinary Medicine, Menufiya University, El-Sadat Branch, Egypt.
10.21608/jvmr.2020.77586
Three vaccination regimes based on Clostridium perfringens (C. perfringens) type A, C or combined AC toxoids were evaluated to detect if toxoid vaccines can prevent necrtotic enteritis (NE) caused by clostridial infection in broilers. The vaccines were administered two times at two weeks interval, then the birds were challenged with virulent strains of C. perfringens type A, C or combined AC. Blood samples were taken after both first and second vaccination doses as well as after challenge. Evaluating parameters included clinical signs, gross intestinal lesions, hemogram [red blood cell count (RBCs), packed cell volume (PCV), hemoglobin (Hb), total leukocytic count (TLC) and differential leukocytic count],serum biochemical assays [total protein (TP), albumin (Alb), globulin (Glob), albumin globulin ratio (A/G), serum activities of alanine amino transferase (ALT), aspartate amino transferase (AST), alkaline phosphatase (ALP) and uric acid (UA) values] and ELISA test for detecting serum antibody titers. Results revealed that affected birds showed marked depression, anorexia, reluctance to move, ruffled feathers and diarrhea. Numbers of chickens with intestinal lesions in immunized challenged groups were greatly fewer than the infected non immunized ones. There was an increase in RBCs, PCV and Hb. TLC decreased in infected non immunized birds and increased in vaccinated ones. Heterophils were increased in infected groups while, lymphocytes decreased. Prominent lymphocytosis was observed in immunized birds. Results of biochemical assays showed that there was a significant increase in TP, Alb, Glob, ALP, ALT, AST and UA and decrease in A/G. Results of ELISA test showed that there was a significant increase in antibody titer after immunization paricularly after the second dose of vaccination. The combined AC toxoid provided the greater antibody titer and best protection followed by toxoid A and finally toxoid C. We concluded that results provide an evidence that immunization of broilers with toxoid vaccines paricularly the combined type AC is safe, welltolerated and can protect broiler chickens against NE after the second booster dose of the vaccine.
Clinicopathological,immunological,studies,toxoid,vaccine,successful,alternative,Clostridial,Infection,Broilers
https://jvmr.journals.ekb.eg/article_77586.html
https://jvmr.journals.ekb.eg/article_77586_ebe61c77be39dcb77ba06b2c639f3707.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
20
1
2010
03
01
Recent formulation for polyvalent Clostridial vaccine
116
121
EN
Roukaia
M. Osman
Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt.
M. M.
Fayez
Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt.
H. A.
EL-Helw
Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt.
A.
EL-Meneisy
eterinary Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt.
10.21608/jvmr.2020.77587
Polyvalent clostridial vaccine has been prepared according to L+ dose of C. perfringens type B and D, C. septicum, C. oedematiens, the optical density of C. chauvoei, and flocculation test of C. tetani. The vaccine has been evaluated in guinea pigs, rabbits and sheep. It gave high protective immunity in guinea pigs in challenge test (100% protection), the sera of vaccinated rabbits gave high titers more than the permissible limit. Sera of vaccinated sheep showed high antibody titer and good immune response which revealed that the vaccine able to protect sheep against clostridial diseases. The recent formulation of polyvalent clostridial vaccine is very useful tool for production of highly antigenic multicomponent clostridial vaccine used for control of different clostridial diseases.
Recent,formulation,polyvalent,Clostridial,vaccine
https://jvmr.journals.ekb.eg/article_77587.html
https://jvmr.journals.ekb.eg/article_77587_b3f3eac9f11eb7268d200714b0c5cdd2.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
20
1
2010
03
01
Preparation of Tetanus toxoid for equine
122
125
EN
H. A.
EL-Helw
Veterinary Serum and Vaccine Research Institute, Cairo, Egypt.
M. M.
Fayez
Veterinary Serum and Vaccine Research Institute, Cairo, Egypt.
Roukaia
M. Osman
Veterinary Serum and Vaccine Research Institute, Cairo, Egypt.
10.21608/jvmr.2020.77588
Tetanus toxoid for equines has been prepared by cultivation of C. tetani into new synthetic medium for production of high potent tetanus toxin which gave high flocculation units [90 Lf (Limit of flocculation)], and high minimum lethal dose (MLD) (600,000). Tetanus toxin was inactivated by formalin, and adsorbed on aluminum hydroxide gel as an adjuvant. It was inoculated into rabbits as a preliminary evaluation for its potency, showing the permissible units allowed to be used for vaccination of horse. The vaccine was tested in horses, which exhibited higher titer of specific antibodies persisted for 8 months post vaccination.
Preparation,Tetanus,toxoid,equine
https://jvmr.journals.ekb.eg/article_77588.html
https://jvmr.journals.ekb.eg/article_77588_da578ed1f5cd619ddcf5413272b356eb.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
20
1
2010
03
01
Comparative immunological studies on some single and combined live attenuated vaccines in poultry
126
133
EN
Hanan
M. El-Zahed
Central Laboratory for Evaluation of Veterinary Biologics, Abbasia, Cairo, Egypt
Susan
S. El-Mahdy
Central Laboratory for Evaluation of Veterinary Biologics, Abbasia, Cairo, Egypt
N. A.
Sherif
Central Laboratory for Evaluation of Veterinary Biologics, Abbasia, Cairo, Egypt
Amal
A. Sayed
Central Laboratory for Evaluation of Veterinary Biologics, Abbasia, Cairo, Egypt
Anhar
Abdel Moety
Central Laboratory for Evaluation of Veterinary Biologics, Abbasia, Cairo, Egypt
10.21608/jvmr.2020.77589
In a trial for comparison between the efficiency of single fowl pox (FP) vaccination and the efficiency of each combined FP and Avian encephalomyelitis (AE) vaccination and simultaneous vaccination with FP and Reo and with FP and Chicken anemia virus (CAV) vaccines our conclusion was that there is no antagonistic reaction between FP virus strain and each AE, Reo and CA viruses strains. In addition, humoral immune response against AE virus strain in case of combined AE+FP vaccination is markedly potent than that in case of single AE vaccination, more over the value of average EID50 of AE virus strain in several batches of combined AE+FP vaccines is significantly higher at P > 0.05 than that in several batches of single AE vaccines. On the other hand, immune response against FP virus strain and Reo virus strain in case of simultaneous vaccination with FP and Reo vaccines is higher than that in case of single FP vaccination and single Reo vaccination. Consequently, it is advisable to use combined live attenuated AE+FP vaccine instead of vaccination with single FP and AE separately. Also, application of simultaneous vaccination with FP and Reo vaccines is advisable as it is proved to be more beneficial than vaccination with each vaccine separately specially in case of that FP vaccine of low potency.
Comparative,immunological,studies,combined,live,attenuated,vaccines,Poultry
https://jvmr.journals.ekb.eg/article_77589.html
https://jvmr.journals.ekb.eg/article_77589_30ef7e2fe9fe73f7886eca74b66001aa.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
20
1
2010
03
01
Genomic identification of Pasteurella multocida isolated from turkey flock in Egypt early 2008
134
141
EN
S.A.
Nassif
Central laboratory for evaluation of veterinary biologics
Hanan
M. Ibrahim
Central laboratory for evaluation of veterinary biologics,Faculty of Agriculture, Ain-Shams University
Zeinab
M. Souror
Central laboratory for evaluation of veterinary biologics,Faculty of Agriculture, Ain-Shams University
Arwa H.
Elnaggar
Central laboratory for evaluation of veterinary biologics,Faculty of Agriculture, Ain-Shams University
Hayam
Farouk
Central laboratory for evaluation of veterinary biologics,Faculty of Agriculture, Ain-Shams University
A. B.
Abd Elrazek
Central laboratory for evaluation of veterinary biologics,Faculty of Agriculture, Ain-Shams University
M. M.
Yousef
Central laboratory for evaluation of veterinary biologics,Faculty of Agriculture, Ain-Shams University
Elham.A.
Elebiary
Central laboratory for evaluation of veterinary biologics,Faculty of Agriculture, Ain-Shams University
10.21608/jvmr.2020.77590
Molecular detection and differentiation of Pasteurella multocida strain involved in a separate fowl cholera outbreak in a turkey flock farm located in El-Menofia Governorate, Egypt early 2008 was investigated. The isolated strain was compared with an Egyptian Pasteurella multocida isolate that was previously isolated from turkey flock during last decade besides four vaccinal strain (A:5, A:8, A:9 and D:2) on phenotypic and genotypic characterization basis. Phenotypically all the strains were similar as all the strains produce non haemolytic colonies on blood agar, and all the strains revealed similar biochemial behaviour. On the other hand, the genomic typing of all the stains using rep-PCR techniques [repetitive BOX elements, enterobacterial repetitive intergenic consensus (ERIC) and repetitive extragenic palindromic (REP) polymerase chain reaction (PCR)] differentiated the six Pasteurella multocida strains into six different profiles. The molecular identity between the Pasteurella multocida 2008 strain and the previously isolated strain was 76.6 % and were ranged from 65.2% to 79.2% with the 4 vaccinal strains. These results reported the continuous mutations of the field Pasteurella multocida strains among poultry flocks in Egypt indicating the urgent need for the frequent and continuous molecular epidemiological investigations of fowl cholera outbreaks in various poultry flocks to detect these new strains and update the fowl cholera vaccines.
Genomic,identification,Pasteurella,multocida,isolated,turkey,flock,Egypt,2008
https://jvmr.journals.ekb.eg/article_77590.html
https://jvmr.journals.ekb.eg/article_77590_94a58cf01c5d5371bf8aab01d2729dbb.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
20
1
2010
03
01
Epidemiological studies on Listeriosis in sheep
141
148
EN
E. E.
Younis
Department of Internal Medicine and Infectious Disease, Faculty of Veterinary Medicine,
Mansoura University, Egypt
A. A
El-Sawalhy
African Union/Interafrican Bureau for Animal Resources (AU/IBAR),
Nairobi, Kenya
Soumaya
E. A.
Animal Health Research Institute, Dokki, Giza, Egypt
M. A. A.
El-Beskawy
Veterinary Teaching
Hospital Faculty of Veterinary Medicine, Mansoura University, Egypt.
10.21608/jvmr.2020.77591
This study was carried out in El-Dakahilia governorate on six flocks at different areas 2448 sheep located with varied ages and with history of nervous manifestation. The Prevalence of nervous manifestations was 4.9% (105 /2448). The case fatality rate and mortality rate were 77.14 % and 3.3 % respectively. The percent of Listeria monocytogenes was 26.66% (8/30). Examination of CSF of diseased and control healthy sheep revealed significance elevation of total cell count, total protein and creatinine cytokinase of diseased than control sheep.
Epidemiological,studies,Listeriosis,sheep
https://jvmr.journals.ekb.eg/article_77591.html
https://jvmr.journals.ekb.eg/article_77591_02979cac727170d0bb71f6e97db36d93.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
20
1
2010
03
01
Studies on Corynebacterium pseudotuberculosis, Staphylococcus aureus and Staphylococcus aureus subsp. anaerobius isolated from sheep skin abscesses in Beni Suef Governorate.
149
153
EN
Samia
I. Afifi
Animal Health Research Institute
10.21608/jvmr.2020.77592
Clinical examination of 380 rearing sheep revealed that 30 animal were suffering from skin abscesses with an incidence of (7.89%). Bacteriological examination of 30 swabs from affected sheep revealed isolation of 30 isolates of C. pseudotuberculosis (48.39%) , 18 isolates of S. aureus (29.03%) and 14 isolates of S. aureus subsp. anaerobius (22.58).The isolated bacteria were identified morphologically and biochemically.The results of animal pathogenicity test showed that C. pseudotuberculosis isolates were 100% pathogenic to guinea pigs and 80% of S.aureus isolates were pathogenic to mice, while all isolates of S. aureus subsp. anaerobius were pathogenic to mice. The dead animals showed haemorrhage and symptoms of septicaemia, C. pseudotuberculosis, S.aureus and S. aureus subsp. anaerobius were reisolated from the dead animals. Antimicrobial sensitivity of C. pseudotuberculosis , S. aureus and S. aureus subsp. anaerobius isolates to some antimicrobial agents which usually used in farms showed that from 90% to 100% of C. pseudotuberculosis isolates were sensitive to erythromycin, amoxicillin, tetracycline ,streptomycin, ampicillin and rifampicin while S. aureus isolates (from 55% to 66%) were sensitive to rifampicin,tetracycline ,erythromycin and streptomycin, while S. aureus subsp. anaerobius isolates were moderately resistant to all used antimicrobial agents.
studies,Corynebacterium,pseudotuberculosis,aureus,Staphylococcus,subsp,anaerobius,isolated,sheep,skin,abscesses,Beni Suef,governorate
https://jvmr.journals.ekb.eg/article_77592.html
https://jvmr.journals.ekb.eg/article_77592_f9f634a9e13e79a90c01fe2917c99748.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
20
1
2010
03
01
Hormonal residues in chicken carcasses
154
162
EN
F. A.
Khalafalla
Department of Food Hygiene, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef
Egypt
E. M.
El-Neklway
Department of Food Hygiene, Animal Health Research Institute, Dokki- Giza, Egypt
Hala A. M.
Ibrahim
Department of Food Hygiene, Animal Health Research Institute, Dokki- Giza, Egypt
10.21608/jvmr.2020.77593
Ninety chicks were experimentally, orally received different doses of estradiol-17β and diethylstilbestrol with ration. Samples were collected from tissues (wings, breast muscles, thigh muscles, skin and fat) and giblets (liver and gizzard) for detection of hormonal residues after 4 and 21 days from the last dose, which proved the presence of such residues in all samples. Effect of temperatures (boiling, roasting and freezing) on hormonal residues of positive samples was evaluated. It was proved that There is no significant variations in reduction of hormonal residues in each of breast and thigh muscles of chickens at (p < 0.05) after boiling, roasting and freezing at - 20˚ C as well as a significant differences was detected in skin and fat samples at (p < 0.05) after boiling and roasting. Public health importance of hormonal residues was discussed.
hormonal,residues,chicken,Carcasses
https://jvmr.journals.ekb.eg/article_77593.html
https://jvmr.journals.ekb.eg/article_77593_7c0ff5349d20856aaf7ce40d93595686.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
20
1
2010
03
01
Antimicrobial residues in some slaughtered food animals
163
168
EN
F. A.
Khalafalla
Department of Food Hygiene, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt.
Fatma H. M.
Ali
Department of Food Hygiene, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt.
K. A.
Abd-Allah
Department of Food Hygiene, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt
10.21608/jvmr.2020.77594
A total of two hundreds of slaughtered animals; 50 each of cattle buffaloes, sheep and buffalo calves were collected from slaughterhouse Giza. Each animal was represented by muscular part, prenephric fat, liver, and kidneys. The Four Plate Technique (FPT) is intended to detect antimicrobial residues in collected samples. The liver samples showed high detection incidences (30, 16, 16 and 8%) in cattle, buffaloe, sheep and buffaloe calves respectively as compared to kidney samples (16, 12, 10 and 6% respectively). The lowest incidence was detected in fat samples (0%) followed by muscle samples (4%). High incidence was detected in medium I followed by medium II and III, finally medium IV as well as medium V which failed to be showed any incidence of detection. The tetracycline residues in tissues of slaughtered animals depend on husbandry as well as on withdrawal time after use. The liver (67 + 15, 50 + 14, 54 + 10 and 3 +0.8) and kidney (63 + 16, 46 + 12, 56 + 12 and 8 + 2) samples in cattle, buffaloe, sheep and buffaloe calves respectively showed high residual levels as compared with muscles and fat. The detection of sulfonamide residues in tissues of slaughtered animals may be attributed to misuse of these compounds for long term in animal feed as well as result in accumulation in animal tissues.
Antimicrobial,residues,slaughtered,food,Animals
https://jvmr.journals.ekb.eg/article_77594.html
https://jvmr.journals.ekb.eg/article_77594_af1fa2184af139d576292bd586a76327.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
20
1
2010
03
01
Bacteriological hazards in camel's meat products
169
174
EN
F. A.
Khalafalla
Department of Food Hygiene, Faculty of Veterinary Medicine, Beni-Suef University
Zienab
M. Niazi
nimal
Health Research Institute, Dokki- Giza, Egypt.
Dalia
Y. Mohamed
Animal
Health Research Institute, Dokki- Giza, Egypt.
10.21608/jvmr.2020.77595
A total of one hundred meat products samples; 20 each of camel's minced meat, burger, rice kofta, frankfurter and luncheon were collected from different supermarkets at Cairo and Giza Cities. All samples were exposed to bacteriological examination and showed that the mean values of aerobic plate count, psychrophiles, coli forms, fecal coli forms, S. aureus, and B. cereus in examined camel's minced meat, burger and rice kofta were higher than luncheon and frankfurter. E. coli, Salmonellae, S. aureus and B. cereus, L. monocytogenes were isolated from examined camel's meat products by different percent. The public health significance of the isolated microorganisms as well as suggestions for improving the quality of the camel meat products were discussed.
Bacteriological,hazards,camel,meat,products
https://jvmr.journals.ekb.eg/article_77595.html
https://jvmr.journals.ekb.eg/article_77595_1e7487c41600bfe53116e8d43a270846.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
20
1
2010
03
01
Bacteriological status of fish marketed in Beni-Suef City
175
180
EN
Fathy A.
Khalafalla
Department of Food Hygiene, Faculty of Veterinary Medicine, Beni-Suef University
Fatma
H. M. Ali
Department of Food Hygiene, Faculty of Veterinary Medicine, Beni-Suef University
Abdel-Rahim
H. A. Hassan
Department of Food Hygiene, Faculty of Veterinary Medicine, Beni-Suef University
10.21608/jvmr.2020.77643
This study was carried out to evaluate the bacteriological status of fresh Tilapia nilotica, fresh Clarias lazera, frozen Mackerel and smoked Herring fish marketed in Beni-Suef City. The collected samples were examined for total bacterial count, coli form (MPN), faecal coli forms (MPN), E. coli (MPN), Staphylococcus aureus count, total proteolytic count, total lipolytic count, Aeromonas count and Pseudomonas count, as well as the isolation of E. coli, Staphylococcus aureus, Aeromonas hydrophila, Pseudomonas aeruginosa, Yersinia enterocolitica and Listeria monocytogenes. Most of the examined samples were within the permissible limits recommended by EOS and ICMSF, few samples of fresh Clarias lazera exceeded the recommended limits. The economic importance and public health significance of the isolated organisms were discussed.
fish,APC,coliforms,tilapia,Clarias lazera
https://jvmr.journals.ekb.eg/article_77643.html
https://jvmr.journals.ekb.eg/article_77643_6838a5bcf45ec4e8f61d0900e82c4280.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
20
1
2010
03
01
Quality and acceptability of value-added beef burger
181
187
EN
F. A.
Khalafalla
Department of Food Hygiene, Faculty of Veterinary Medicine, Beni-Suef University
Fatma H. M.
Ali
Department of Food Hygiene, Faculty of Veterinary Medicine, Beni-Suef University
A. H.
Abdel-Azeem
Department of Food Hygiene, Faculty of Veterinary Medicine, Beni-Suef University
Gehan M. A.
Kassem
Department of Food Hygiene and Control, Faculty of Veterinary Medicine, Cairo University, Giza,
Egypt.
M. M. T.
Emara
Department of Food Hygiene and Control, Faculty of Veterinary Medicine, Cairo University, Giza,
Egypt.
10.21608/jvmr.2020.77598
The sensory quality attributes of coated and uncoated beef burger patties formulated with texture soy granules or vegetables (peas and carrots) were studied in comparison to that of the control ones. Incorporation of textured soy granules significantly reduced the color, marbling, appearance, flavor, tenderness, juiciness, taste and overall acceptability in comparison with either control or vegetable extended burger. Addition of peas and carrots to uncoated burger significantly reduced the binding scores in raw samples, as well as flavor and juiciness in cooked samples, however, no significant differences could be observed in the other sensory attributes in both raw and cooked products. Vegetable extended burger had the highest cooking loss percent (20.14), followed by control samples (17.83), while soy extended product had the lowest value (15.82%). Application of batter and breading to vegetable extended burger significantly improved the investigated sensory parameters in comparison with the uncoated samples. On the other hand, application of batter and breading to soy extended burger revealed no improvement in the sensory quality attributes in both raw and cooked samples. Addition of soy granules and vegetables significantly increased the moisture, ash and carbohydrate and reduced the fat content of raw burger patties. Moreover the incorporation of textured soy significantly increased the protein content.
Quality,acceptability,value,beef,burger
https://jvmr.journals.ekb.eg/article_77598.html
https://jvmr.journals.ekb.eg/article_77598_87c5cfbb29b72c80383492e9365c6fd3.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
20
1
2010
03
01
Quality assurance of yoghurt during processing
188
194
EN
Saadia H. H.
El- Shinawy
Department of Food Hygiene, Faculty of Veterinary Medicine, Beni-Suef University
A. M.
El Kholy
Department of Food Hygiene, Faculty of Veterinary Medicine, Beni-Suef University
M. M. A.
Zeinhom
Department of Food Hygiene, Faculty of Veterinary Medicine, Beni-Suef University
10.21608/jvmr.2020.77599
The present study was carried out to determine the critical points during processing of yoghurt, through examination of Acidity % and Storch's test to detect the efficiency of heat treated milk samples and Microbiological examination of samples collected weekly over a period of 7 months for TCC, Thermoduric, Total coliform, Faecal coliform, E. coli, Enterococci, S. aureus, Yersinia enterocolitica and Total yeast and mould counts. A total of 100 samples of raw milk, heat treated milk, inoculated milk and yoghurt (25 of each) in addition to 75 samples from worker’s hands, plastic packages and mixing vat (25 of each) were collected under strict hygienic conditions, also twenty five samples of yoghurt at the end of the expire date were collected and examined microbiologically. The obtained results concluded that the major sources of yoghurt contamination were raw milk, improperly cleaned and sanitized worker’s hands as well as the added starter culture. Finally, the public health and economic importance of the isolated organisms were mentioned.
Quality,assurance,yoghurt,processing
https://jvmr.journals.ekb.eg/article_77599.html
https://jvmr.journals.ekb.eg/article_77599_414ee40e947ce48a273bd3b33457eaca.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
20
1
2010
03
01
Potentials of human exposure to Listeria spp. from dairy cattle
195
202
EN
A. E.
Abdel-Ghany
Department of Hygiene, Management and Zoonoses, Faculty of Veterinary Medicine, Beni-Suef
University, Beni-Suef, Egypt.
M. A.
Ibrahim
Department of Hygiene, Management and Zoonoses, Faculty of Veterinary Medicine, Beni-Suef
University, Beni-Suef, Egypt
10.21608/jvmr.2020.77600
This study was performed in the period February 2009 through January 2010 to determine the role of dairy cattle in transmitting listeriosis to man in Beni-Suef Governorate, Egypt. Individual milk samples and rectal swabs were gathered from 175 dairy cows (125 clinically diseased and 50 apparently healthy). A total of 75 kariesh cheese and 150 dairy shop milk samples were randomly collected from the same localities where the examined cattle were reared. Stool and blood samples were taken from 125 humans comprising 75 individuals residing in close contact with the examined cows and 50 feverish inpatients. The occurrence of Listeria spp. in the examined dairy cattle revealed that 1.14 % of individual milk samples harboured Listeria spp.; L. innocua (0.57 %) and L. seeligeri (0.57 %). None of rectal swabs revealed a positive result. L. monocytogenes could not be recovered from any of the examined cattle samples. Examination of kariesh cheese demonstrated a positive result to L. innocua (1.33 %). Concerning dairy shop milk examined, 5.33 % was Listeria spp.-positive; they were identified as L.monocytogenes (2.67 %), L. innocua (1.33 %) and L.seeligeri (1.33 %). Examination of humans revealed a positive result for L. welshimeri in a stool sample (0.8 %) taken from an apparently healthy woman while all the examined blood sampleswere Listeria-negative. It was concluded that listeriosis in Beni-Suef Governorate appears to be ofsporadic nature and that the potential of human exposure to Listeria spp. and L. monocytogenes from dairy cattle is more likely to exist in dairy shop milk rather than being related to the animal itself.
Potentials,human,exposure,Listeria spp,dairy,cattle
https://jvmr.journals.ekb.eg/article_77600.html
https://jvmr.journals.ekb.eg/article_77600_a8186a41cf2678c08abc32d1add3fc12.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
20
1
2010
03
01
Assessment of defined antigen cocktails for sero-diagnosis of tuberculosis
203
208
EN
Y. A.
Soliman
Central Laboratory for Evaluation of Veterinary Biologics
M.
Abdel-Rahman
Veterinary Serum and Vaccine Research
Institute, Abbasia, Cairo, Egypt.
Afaf A.
Khedr
Central Laboratory for Evaluation of Veterinary Biologics
E. N.
Amin
Veterinary Serum and Vaccine Research
Institute, Abbasia, Cairo, Egypt.
M. A.
Makharita
Central Laboratory for Evaluation of Veterinary Biologics
10.21608/jvmr.2020.77601
To achieve a better diagnostic assay for the bovine tuberculosis, antigen cocktail composed of Ag85; rESAT-6 and rMPB-70 protein antigens were compared to the bovine tuberculin using ELISA test. The antigen cocktail could detect 80 % of the tuberculin negative cattle showed lesions in the post mortem examination compared to 60% with the PPD-B. For the tuberculin positive cattle, the results were 70% and 80% for the antigen cocktail and PPD-B respectively. On the other hand the antigen cocktail gave only 26.6% in sera of the tuberculin positive animals with non visible lesions compared to 53.3% with the PPD-B. 70% of the tuberculin negative cattle with localized lesions in the P/M examination reacted with the antigen cocktail compared to 50% only with the PPD-B. The results concluded the ability of the antigen cocktail to overcome the false negative results obtained with the tuberculin test and to detect the active recent infection of cattle which allow the rapid eradication of the infected animals from herds before the spread of the disease.
Assessment,defined,antigen,cocktails,Sero-diagnosis,tuberculosis
https://jvmr.journals.ekb.eg/article_77601.html
https://jvmr.journals.ekb.eg/article_77601_de434a586b03aec6b36e3ad54a494e55.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
20
1
2010
03
01
The influence of some molluscicides on reproductive activity in rats
209
214
EN
S. S.
Ibrahim
Department of Physiology, Faculty of Veterinary Medicine, Beni-Suef University
A.
Aboul-Ela
Department of Physiology, Faculty of Veterinary Medicine, Beni-Suef University
E. A.
Mabrouk
Department of Physiology, Faculty of Veterinary Medicine, Beni-Suef University
G. A.
Taha
Department of Physiology, Faculty of Veterinary Medicine, Beni-Suef University
10.21608/jvmr.2020.77602
The present study is an endeavor for profound exploration about the effects of 2 commonly used molluscicides in Egypt (bayluscide and copper sulfate ) on some reproductive aspects in mature male Albino rats. For this purpose, 180 mature male Albino rats were used. Animals were equally divided into 3 groups; the 1st group was considered as controls administered distilled water while those of the 2nd and 3rd groups were administered distilled water containing 1 / 20 LD50 of either baylucide or copper sulfate every 3 days for 8 successive weeks. The results of the present study revealed that administration of either molluscicides induced a serious drastic decline in the levels of the reproductive hormones; an effect which was not completely reversed after removal of the molluscicide for 8 successive weeks. This disruption upon reproductive hormones was concomitant with a significant deviation of semen parameters represented by a drop in the individual motility concomitant with an increase in dead sperm percentage as well as total sperm abnormalities. Moreover, stoppage of molluscicides administration along 8 successive weeks failed to improve all studied semen parameters. Moreover, evaluation of total antioxidant activity disclosed that application of either bayluscide or copper sulfate resulted in significant reduction in the total antioxidant capacity as compared with the corresponding control values; a finding which persisted after prevention of molluscicides administration along 8 weeks.
Influence,molluscicides,reproductive,activity,Rats
https://jvmr.journals.ekb.eg/article_77602.html
https://jvmr.journals.ekb.eg/article_77602_3d1b144d2d3f94b77d50e5c789502a66.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
20
1
2010
03
01
Influence of probiotic on serum biochemical profile, growth performance and immunostatus in broiler chickens
215
221
EN
M. A.
Abonorag
Animal Health Reaserch Institute
Elham A.
Mobarez
Animal Health Reaserch Institute
Y. A.
Elkatan
Animal Health Reaserch Institute
Sahar A.
Galal
Animal Health Reaserch Institute
Nahla Sh.
Kotb
National Organization for drug Control and research
10.21608/jvmr.2020.77603
A total of 90 one-day-old male broiler chicks were fed on balanced ration for 15 days and then divided into 3 equal groups. Control group fed ration probiotic free, group 2 and 3 fed ration supplemented with probiotic at concentration levels 0.5 and 1.0g/kg ration respectively for 4 weeks.It was found that probiotic improve body weight gain and feed conversion ratio. Relative organs weights were not altered in groups of birds fed ration mixed with probiotic. Serum values of biochemical parameter (AST, ALT, ALP, total protein, A/G ratio, Ph) were not significantly changed in comparison to control group. Probiotic enhance the immune response of broiler chickens in a dose dependent relation as documented by increasing the serumNDHI antibody geometric mean titres to ND vaccine, phagocyte percentage and phagocytic index of peripheral blood monocyte of broiler chickens. We concluded that probiotic composed of 3 strains of bacteria (Pediococcus acidilactici, Pediococaoccus pentosaceus and Bacillus Amylolique- faciens) in addition to 2 strains of yeast( Pichia farinose and Dekera anomala ) enhance body weight gain, feed conversion, growth performance and Newcastle disease antibody titres in broiler chickens. Moreover, probiotic was safe as proved by serum biochemical profile and relative organ weight of male broiler chickens.
Influence,probiotic,serum,biochemical,profile,Growth,performance,immunostatus,broiler,chickens
https://jvmr.journals.ekb.eg/article_77603.html
https://jvmr.journals.ekb.eg/article_77603_db283f183828e38f1ed6b7a6d0d5d263.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
20
1
2010
03
01
Biosorption effect of olive mill on heavy metal levels in serum and tissues of albino rats
222
226
EN
Omima
I. Ali
Department of Biochemistry, Animal Health Research Institute, Dokki, Giza, Egypt.
Sahar M.
Srour
Department of Biochemistry, Animal Health Research Institute, Dokki, Giza, Egypt
10.21608/jvmr.2020.77604
Two various kinds of olive mill solid residues (Crude and partly destoned) were used to investigate the adsorbing property of olive mill solid residues (OMSR) to heavy metals. 100 adult albino rats were classified into four groups. One group served as (–ve) control group (10 rats), received balanced ration and supplied tap water; in addition to three equal groups (each of 30 rats) received water polluted with1/100LD50 of either lead, cadmium or copper at concentration levels( 107.2 mg/L, 8.8mg/L or 58.4mg/L respectively). Each main group was divided into 3sub- groups, one (+ve) control and two experimental groups fed either 20 % crude olive mill or 20% partly destoned olive mill. Polluted water administration extended for 1 month, meanwhile feeding 20% crude or partly destoned (OMSR) continued for further 30 days after cessation of polluted water as a withdrawal period. Samples of serum, muscle, liver and kidney were collected at one month of the experiment and at the end of the withdrawal period after one month. Level of metals were determined by atomic absorption spectrophotometer. Results indicate that crude olive mill exhibit higher adsorption capacities to Pb, cd, and cu than partly destoned especially at withdrawal period. In conclusion, the main advantage of this process is the conversion of this waste product to a useful adsorbent material with low coast in minimizing the toxic hazard of environmental pollution with heavy metals.
Biosorption,Effect,olive,mill,heavy,metal,levels,serum,tissues,albino,Rats
https://jvmr.journals.ekb.eg/article_77604.html
https://jvmr.journals.ekb.eg/article_77604_ced2dcd40d75f9cdb85df0bfd900013b.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
20
1
2010
03
01
Effect of some neurotransmitters on the testes and reproductive hormones in albino rats
227
231
EN
E. A.
Mabrouk
Deptartment of Physiology, Faculty of Veterinary Medicine, Beni-Suef University
M. D.
Ismail
Deptartment of
Animal Medicine, Faculty of Veterinary Medicine, South Valley University
A. M.
Mohammed
Department of
Physiology, Faculty of Veterinary Medicine, South Valley University
10.21608/jvmr.2020.77605
The present study has been carried out to investigate the effect of three neurotransmitters (Glutamate, L-Arginine and GABA) on some aspects of the reproductive performance of mature male Albino rats. For this purpose, a total of 100 mature male Albino rats were used. Rats were divided into 4 comparable groups; the first consists of 10 rats, was left as control. The second was administered glutamate 10 mg/ kg, the third group was injected by L- Arginine 20 mg/ kg while the fourth was injected by GABA 1 mg / rat. The results showed that administration of glutamate was concomitant with increase in synthesis and release of pituitary LH causing increase in its serum level as well as decrease serum level of testosterone. On the other hand, prolonged L-Arginine administration led to remarkable elevation in both pituitary and serum LH and significant decrease of serum testosterone. While, GABA administration led to remarkable decrease in pituitary and serum LH with significant decrease in serum testosterone level.
Effect,neurotransmitters,testes,reproductive,Hormones,albino,Rats
https://jvmr.journals.ekb.eg/article_77605.html
https://jvmr.journals.ekb.eg/article_77605_82d55b063c08a5c9902beba4ee3f11a9.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
20
1
2010
03
01
Some studies on Listeria Monocytogenes infection in buffaloes
232
235
EN
Magda
F. Essa
Department of Buffalo Diseases, Animal Health Research Institute, Dokki, Giza, Egypt.
Hanan
K. Mahmoud
Department of Buffalo Diseases, Animal Health Research Institute, Dokki, Giza, Egypt.
10.21608/jvmr.2020.77606
The present study concerned with Listeria monocytogenes which were isolated from apparently healthy buffaloes, buffaloes suffered from abortion, bedding and silage, which were collected from different localities. From the total examined samples (375), (300 samples from apparently healthy buffaloes, bedding and silage, also 75 samples from buffaloes suffered from abortion , bedding and silage) the prevalence of L. monocytogenes was 11(14.7%), isolates from buffaloes suffered from abortion, bedding and silage. All isolates were motile at room temperature, also exhibited positive CAMP test and exhibited narrow zone of β-hemolysis. Serological identification of isolates revealed 6(54.5%) were serotype 4 while 5(45.5%) were untyped. There were differences in isolates susceptibilities to different anti-microbial agents. L. monocytogenes had 100% sensitivity to Ampicillin, chloramphenicol, erythromycin, tetracycline and ceftiofur sodium. Virulence of isolates was assayed by I/P inoculation of 109 CFU in mice, all inoculated mice died within 1-5 days. PCR assay for L. monocytogenes isolates revealed positive amplification of 827 bp fragment of act A gene.
studies,Listeria,Monocytogenes,Infection,buffaloes
https://jvmr.journals.ekb.eg/article_77606.html
https://jvmr.journals.ekb.eg/article_77606_81aaf5f897789b97afe9c0f3ff47b054.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
20
1
2010
03
01
Antimicrobial activity of some cephalosporins with special reference to their effects on body weight and immune response to Newcastle disease vaccine in fayoumy chicks
236
242
EN
I. A.
Radwan
Microbiology Department, Faculty of Veterinary Medicine, Beni-Suef
University, Beni-Suef, 62511, Egypt.
Abeer M.
Radi
Pharmacology Department, Faculty of Veterinary Medicine, Beni-Suef
University, Beni-Suef, 62511, Egypt.
10.21608/jvmr.2020.77607
The susceptibility of the most common bacterial pathogens, namely E. coli, P. mirabilis and Ps. aeroginosa which were isolated from egg incubators and yolk sacs of randomly selected one day old Fayoumy chicks to three selected cephalosporins (cephradine, ceftiofur and cefquinome) were studied. The minimum inhibitory concentration (MIC), the minimum bactericidal concentration (MBC) of the tested drugs and the effect of these antibiotics on the body weight gain, mortality and immune response against Newcastle disease (ND) vaccine of the same bread of chicks were also estimated. The tested organisms were sensitive to ceftiofur and cefquinome whereas E.coli and ps. aeroginosa were found to be resistant to cephradine. The results showed that mortalities were higher in control and cephradine treated groups, while it was lower in the ceftiofur and cefquinome treated groups. On the other hand, the lowest mean body weight was recorded in control group (155.7±6.55 gm) followed by ceftiofur treated group (162.5±2.06 gm) and the highest mean body weight was recorded in cefquinome treated group (183.5±1.66 gm, p < 0.01) at 30 days of age. The study revealed that the tested antibiotics not exert any immune suppressive effect against (ND) vaccine.
Antimicrobial,activity,cephalosporins,special,reference,Effects,body,weight,Immune,Response,Newcastle,Disease,vaccine,fayoumy,chicks
https://jvmr.journals.ekb.eg/article_77607.html
https://jvmr.journals.ekb.eg/article_77607_498931aa451b3097acce289958d60edb.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
20
1
2010
03
01
Some studies on Corynebacterium pseudotuberculosis causing Oedematous Skin Disease in Egyptian buffaloes
243
246
EN
Magda
F. Essa
Department of Buffalo Diseases, Animal Health Research Institute, Dokki, Giza, Egypt.
10.21608/jvmr.2020.77608
Out of 63 bacteriologically examined sanguineous fluids samples which were collected from Oedematous Skin Disease (O.S.D.) lesions, 37 isolates of C. pseudotuberculosis (C.p.) were recovered. The sensitivity tests revealed that isolates were highly sensitive to trimethoprim + sulphamethoxazole, amoxycillin, gentamicin and enrofloxacin. Diphtheria toxin (DT) produced by C. pseudotuberculosis of buffalo was determined by using the double immunodiffusion technique, it was applied on concentrated exotoxins which was prepared from C.p. isolates against Diphtheria toxin antiserum, its results were 15 (40.54%) positive and 22 (59.46) %) negative to presence of DT. Virulence of isolates having only phospholipase D ( PLD) or both PLD and DT was assayed by S/C inoculation of exotoxines prepared from isolates in guinea pigs, 15(40.54%) guinea pigs died within 18 hours while 22 (59.46%) guinea pigs died during 48 hours. An important point in this investigation that there have been very rare previous reports describing the production of DT by local isolates of C. pseudotuberculosis of buffalo.
studies,Corynebacterium,pseudotuberculosis,Oedematous,skin,Disease,Egyptian,buffaloes
https://jvmr.journals.ekb.eg/article_77608.html
https://jvmr.journals.ekb.eg/article_77608_6fbc2b66e0510b9c72132dae5c6c472b.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
20
1
2010
03
01
Field study on the effect of aluminum silicate adsorbent on performance of 51 weeks old broiler breeder chickens
247
252
EN
G. A.
Zohair
Department of Animal Production, Faculty of Agriculture, Sana'a University, Yemen
M. M.
Amer
Department
of Poultry Diseases, Faculty of Veterinary Medicine, Cairo University, Egypt
A. E.
Hanafei
El-Watania
Poultry Company, Giza, Egypt
10.21608/jvmr.2020.77609
In 9 weeks field study, a total of 14100 (Ross broiler breeders) 51 weeks-old chickens fed on the same ration, placed in 2 houses (6600 female + 450 male / house). Birds of house 1 were treated with antimycotoxin adsorbent aluminum silicate (G-V-tox®) 5 kgm/ ton, while those of house 2 were kept as non treated controls. Productivity and reproductivety parameters were calculated for comparison. Treated flock showed improved average egg production (Average 62.2%/week) compared with non treated (Average 61.7%/week), but all still lower than farm stander (Average 76.4%/week). Marked improvement was in the 1st 3 weeks (51-53) of treatment only. Total 9 weeks production declined was 5.5% and 8.4% in control and silicate treated flock; with weekly average of 0.61, and 0.93; respectively. Control flock was slower in decline of production (0.61%/week) than treated flock (0.93%/week). Average weekly egg production and hatching eggs/ hen in treated flock was lower than standard and higher than non treated. Hatchery parameters of treated were improved in treated at the first 3 weeks post treatment. The fertility was higher in aluminum silicate treated group (77.2%), than the untreated one (72.19%). The hatchability was in silicate treated (63.66%) versus (62.25%) in the untreated control. Culls % in hatched chicks was 1.91% in treated flock and lower than in non treated (2.85%). Difference percentage between fertility and hatchability of G.V. tox treated chickens was (10.84%) higher than untreated control (16%). The number of marketable chicks l100 was also improved in treated than non treated. In conclusion, our field study cleared that administration of Silicate in ration for treatment of broiler breeders resulted in an improved production and hatchery performance as compared with non medicated control. However; it did not restore it to the farm stander. Consequently the results indicated that we still in need for more effective products to be used to control mycotoxins in breeder chicken.
field,study,Effect,Aluminum,silicate,adsorbent,performance,51,weeks,old,broiler,breeder,chickens
https://jvmr.journals.ekb.eg/article_77609.html
https://jvmr.journals.ekb.eg/article_77609_647f97050da98565fd7c40c0bdaa81a0.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
20
1
2010
03
01
Campylobacter infection in broiler breeder flocks in El-Minia governorate
253
259
EN
A. M.
Youssif
Department of Bacteriology, Animal Health Research Institute, El-Minia Branch, El-Minia, Egypt
10.21608/jvmr.2020.77610
Existence of Campylobacter species that colonize broiler chickens intestinal tract raised and slaughtered in El-Minia Governorate as a source of animal protein origin for human consumption were studied. Samples were collected from 381 broiler chickens from different private farms distributed in six cities related to El-Minia Governorate and examined for the prevalence of Campylobacter spp. Percentage of Campylobacter jejuni isolation was 19.5% , 14.8% , 18.3% , 14.7% , 12.3% and 12.1% from Maggagha, Beni-Mazar, Samalout, Abou-Curcas, Mallawey and Deer-Mouase, respectively. While Campylobacter coli was 4.8% , 6.2% , 4.3% , 6.7% , 3.5 % and 0.0% respectively . Higher rate of isolation of Campylobacter jejuni 18.0% and Campylobacter coli 5.1% were obtained from 216 freshly dead broiler chickens carcasses than 165 diseased and slaughter birds which revealed 13.3% and 4.2% , respectively . Overall , Campylobacter spp. showed a higher tissue affinity for caecum (11.3%) Campylobacter jejuni and (2.6%) Campylobacter coli than for jejunum (4.2%) C. jejuni and (1.6%) C. coli and liver (1.6%) C.jejuni and (1.6%) C. coli. Susceptibilities of the recovered Campylobacter jejuni and C.coli, isolates to fifteen antibiotic discs clarified that gentamycin, neomycin, chloramphenicol and nalidixic acid in addition most of the strain were resistant to penicillin , ampicillin, tetracycline , sulfa methoxazole , trimethoprim, cephalothin and enerofloxacin . Public health hazard of enteropathogenic campylobacter was discussed and suggestive measures for reduction of campylobacter in broiler chicken farms were explained.
Campylobacter,Infection,broiler,breeder,flocks,El-Minia,governorate
https://jvmr.journals.ekb.eg/article_77610.html
https://jvmr.journals.ekb.eg/article_77610_ebec2e115697bf67ac82020d1af47201.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
20
1
2010
03
01
Efficacy of living attenuated Salmonella typhimurium vaccine in poultry
260
262
EN
Hanan
M. Ibrahim
Central Laboratory for Evaluation of Veterinary Biologics, Abbasia, Cairo, Egypt.
Zeinab
M. Souror
Central Laboratory for Evaluation of Veterinary Biologics, Abbasia, Cairo, Egypt.
N.A.
Sherif
Central Laboratory for Evaluation of Veterinary Biologics, Abbasia, Cairo, Egypt.
M. M.
Lotfy
Central Laboratory for Evaluation of Veterinary Biologics, Abbasia, Cairo, Egypt.
Elham
A. El Ebiary
Central Laboratory for Evaluation of Veterinary Biologics, Abbasia, Cairo, Egypt
M. A.
Makharita
Central Laboratory for Evaluation of Veterinary Biologics, Abbasia, Cairo, Egypt.
10.21608/jvmr.2020.77611
The main goal of this study was to evaluate the protective efficacy of living attenuated Salmonella Typhimurium (S. Typhimurium) vaccine in poultry and to evaluate its use in control of salmonella infection in chickens. Oral vaccination of chickens within 36 hours after hatching and after 6 weeks from the first dose induced a strong humoral immune response as measured by ELISA. Challenge test was done with virulent strain of S. Typhimurium. Shedding of S. Typhimurium was detected during the first day, but after 14 days, salmonella could not be detected in the internal organs of vaccinated chickens compared with the non-vaccinated challenged chickens.
Efficacy,living,attenuated,Salmonella,typhimurium,vaccine,Poultry
https://jvmr.journals.ekb.eg/article_77611.html
https://jvmr.journals.ekb.eg/article_77611_f6e587cf27abec57e9d4a8fff382e1c6.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
20
1
2010
03
01
Trial for preparation and evaluation of combined vaccine against ND, IB and M. gallisepticum diseases in chickens
263
267
EN
S. S.
Salama
Central Laboratory for Evaluation of Veterinary Biologics
Eman
A. Hasan
Veterinary Serum and Vaccine
Research Institute, Abbasia, Cairo
Hanan E.
Mohammed
Central Laboratory for Evaluation of Veterinary Biologics
Eman
S. Ahmed
Veterinary Serum and Vaccine
Research Institute, Abbasia, Cairo
Nadia
Ebrahim
Veterinary Serum and Vaccine
Research Institute, Abbasia, Cairo
Susan
S. El-Mahdy
Central Laboratory for Evaluation of Veterinary Biologics
10.21608/jvmr.2020.77612
In this study, a combined Trivalent vaccine against ND, IB and M. gallisepticum was locally prepared and evaluated in comparison with other locally prepared Bivalent ND and IB and monovalent M. gallisepticum vaccines. The obtained results were promising for this locally prepared Trivalent vaccine and the immune response was outstanding starting at the 2nd week post vaccination and showed extended raising allover the experiment period. The immune response of chickens vaccinated with the Trivalent was shoot up post boostering at the 8th week post 1st vaccination. These results were confirmed and supported by the challenge tests using the virulent strains of the three pathogens. So it could be recommend that the production of this Trivalent ND, IB and M. gallisepticum will help in the control of the three diseases and their complications.
Trial,Preparation,Evaluation,combined,vaccine,against,ND,IB,M. gallisepticum,diseases,chickens
https://jvmr.journals.ekb.eg/article_77612.html
https://jvmr.journals.ekb.eg/article_77612_f5acff7c61decb745c9b060245d7d618.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
20
1
2010
03
01
Studies on maternal antibodies to avian influenza H9N2 vaccine
268
274
EN
M. M.
Amer
Poultry Diseases Department, Faculty of Veterinary Medicine, Cairo University
A. S.
Hamouda
Poultry Diseases Department, Faculty of Veterinary Medicine, Cairo University
K. M.
EL-Bayomi
Poultry
Diseases Department, National Research Center, Dokki, Giza
10.21608/jvmr.2020.77613
Broiler breeder Lohmann chickens aged 39 weeks received 3 doses each 0.2 ml of the inactivated oil emulsion AI- H9N2 vaccine, at the 2nd, 7th and 15th weeks of age by subcutaneous injection. The individual HI values of the tested samples were homogenous as their SD values were lower. All breeder and progeny sera were positive (100- 66.7%) at weeks 40- 46 weeks of age. Correlation between parents and progeny HI antibody levels was 0.95. Progeny/Parents HI antibodies percentage were ranged from 54.9 to 65.2%. Correlation between parents and progeny ELISA and HI antibody levels were 0.91 and 0.60- 0.65; respectively. The detected HI antibody titres at the 3rd day of age were slightly increased than that of the 1st day titres followed by gradual decrease to be apparently negative at the 12th -21st day of age in comparison to the original levels. The tested groups for Antibodies to H9 by ELISA test were still detected to 21- 27 days of age of progeny. The half-life time of maternal antibodies expressed as loss of one HI log 2 between groups was ranged from 3.3- 7.2 days; with average 5.1- 5.6 days. Half life time by ELISA titre was in average of 8.9 days. Correlations between HI and ELISA ranged from 0.83-0.94. We concluded that both HI and ELISA tests are of the same value in detection of AI antibodies and first vaccination of broiler chicks with maternal antibodies against AI H9N2 must be done after the 6th day of age.
studies,maternal,antibodies,avian,influenza,H9N2,vaccine
https://jvmr.journals.ekb.eg/article_77613.html
https://jvmr.journals.ekb.eg/article_77613_0d41670fc423d0a56b5b8a1777e85ed6.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
20
1
2010
03
01
Immunomodulating and zootechnical effect of some bacterial components on broiler chicken vaccinated with Newcastle disease vaccine
275
279
EN
M. F.
El-Kady
Department of Poultry Diseases
S. M.
Tamam
Department of Virology, Faculty of Veterinary Medicine,
Beni-Suef University
Azza A.
El Sawah
Department of Poultry Diseases
A.
Okasha
Department of Poultry Diseases
10.21608/jvmr.2020.77614
This study was carried out to evaluate the immunomodulating effects of, inactivated cells of Propionibacterium acnes and cell wall lipopolysaccharide (LPS) of the a pathogenic E. coli (INMUNAIR® 17.5) 0.5ml/ L and 1-3, 1-6 β-glucans (BETAPOLO®) 1 ml / L on the immune response of chickens to Newcastle disease (ND) vaccine. The results showed that administration of IMR before vaccination was resulted in food conversion rate (FCR) higher than after vaccination . Significantly higher NDV HI antibody titers in IMR and Betapolo medicated groups as compared with control groups which in turn induce high protection rate in challenge test .Thymus, spleen and bursal indices of control negative showed significantly lower values than vaccinated medicated and non- vaccinatedmedicated groups (P≤ 0.05).
Immunomodulating,zootechnical,Effect,Bacterial,components,broiler,chicken,vaccinated,Newcastle,Disease,vaccine
https://jvmr.journals.ekb.eg/article_77614.html
https://jvmr.journals.ekb.eg/article_77614_7f14ab688b00c00db96dd412b307ec47.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
20
1
2010
03
01
Phenotypic and genetic characterization of fowl pox and turkey pox viruses
280
285
EN
Olfat
E. Nakhla
Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo
Y. A.
Soliman
Central Laboratory for
Evaluation of Veterinary Biologics, Abbasia, Cairo, Egypt
M. M.
Taha
Central Laboratory for
Evaluation of Veterinary Biologics, Abbasia, Cairo, Egypt.
10.21608/jvmr.2020.77620
Fowl and turkey pox viruses were analyzed for their heterogeneity on the basis of protein profile, western blotting, PCR analysis and restriction endonuclease analysis. On the protein level, only a single band with a WM of 115KDa was seen with turkey pox virus but not with the fowl strain, and a band reacted at 10KDa with Turkey but not fowl pox virus in western blotting analysis other protein profiles were nearly the similar. PCR amplification of the lateral terminal repeat (LTR) region of both viruses confirmed a single band migrating about 900 bp with both strains and restriction digestion proved the homology between both strains.
https://jvmr.journals.ekb.eg/article_77620.html
https://jvmr.journals.ekb.eg/article_77620_1e01f6539dc101e8a8f2081b59672ac2.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
20
1
2010
03
01
Comparative studies on anti-avian sera conjugated with fluorescin isothiocyanate
286
289
EN
M. H.
Khodeir
Veterinary Serum and Vaccine Research Institute, Cairo
Ghada
M. El-Sadek
Central Laboratory for Quality
Control of Veterinary Biologics, Cairo, Egypt.
Elham
A. El-Ibiary
Central Laboratory for Quality
Control of Veterinary Biologics, Cairo, Egyp
10.21608/jvmr.2020.77621
Rabbit antisera were successfully prepared against chickens; turkey; ducks; geese; pigeons and quails as antispecies and conjugated with fluorescein isothiocyanate (FITC). The ability to use these antisera as homologous and heterologous antispecies was studied through the application of indirect fluorescent antibody technique (FAT) using specific antigens and antibodies of Newcastle; infectious bursal disease virus; duck plague virus; duck hepatitis virus; fowl cholera and pigeon paramyxo virus. The results showed that Titration of the prepared anti avian sera conjugated with FITC induced strong positive FAT reactions up to dilutions of 1:10000; 1:1000; 1: 100000; 1: 1000; 1: 100000 and 1:1000 for anti- chicken; anti-Turkey; ant-duck; ant-goose; anti-pigeon and antiquill sera respectively. It was found that homolgous species anti-sera showed strong positive FAT reaction (green apple fluorescing) scored as 4+ while hetrologous species anti-sera showed less degree of positive reactions ranged from 3+ to ± reaction showing that the usefulness of anti-sera as reagents in serological techniques is limited by the homogeneity and heterogeneity of such antisera. So, the present preparations could be used to low extent as heterologous anti-avian sera.
Comparative,studies,anti-avian,sera,conjugated,fluorescin,isothiocyanate
https://jvmr.journals.ekb.eg/article_77621.html
https://jvmr.journals.ekb.eg/article_77621_31506855e3646d372441341604a50509.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
20
1
2010
03
01
Antiviral activity of Curcuma longa against Newcastle disease virus (in vitro and in vivo studies)
290
295
EN
S. M.
Tamam
Department of Virology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt.
H. M.
Madbouly
Department of Virology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt.
Fadwa
Amin
Department of Virology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt.
10.21608/jvmr.2020.77624
The antiviral activity of Curcuma longa against NDV on vero cell culture and in infected chicken was observed. The obtained results showed that low concentration of Curcuma longa 0.25% did not produce cytotoxicity on the vero cell. The effect of Curcuma longa on NDV titre was studied and the results revealed clearly that chickens treated with 1% Curcuma longa as Prophylactic also showed higher protection rate (90%), the chickens that treated with 0.5 % Curcuma longa showed protection rate (80%).Chicks that infected with NDV without treatment with Curcuma longa showed lower protection rate (8%). The chickens infected then treated with 1% Curcuma longa showed protection (65%) and the chickens infected then treated with 0.5% Curcuma longa showed protection (40%).
Antiviral,activity,Curcuma,longa,against,Newcastle,Disease,virus,Vitro,Vivo,studies
https://jvmr.journals.ekb.eg/article_77624.html
https://jvmr.journals.ekb.eg/article_77624_d34b8f2aeb4e3e075c7aee095273eff1.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
20
1
2010
03
01
Immunomodulating effect of B-glucans and mannan oligosaccharide on broiler chicks vaccinated with Newcastle disease virus
296
302
EN
S. M.
Tamam
Department of Virology, Faculty of Veterinary Medicine,
Beni-Suef University, Beni-Suef, Egypt.
M. F.
El-kady
Department of Poultry diseases, Faculty of Veterinary Medicine,
Beni-Suef University, Beni-Suef, Egypt.
Azza A.
El Sawah
Department of Poultry diseases, Faculty of Veterinary Medicine,
Beni-Suef University, Beni-Suef, Egypt.
A.
Okasha
Department of Poultry diseases, Faculty of Veterinary Medicine,
Beni-Suef University, Beni-Suef, Egypt.
10.21608/jvmr.2020.77626
This study was carried out to determine the immunomodulating effect of β-glucans and mannan oligosaccharide (MOS) on the immune response of chickens to Newcastle disease vaccine. The results showed that birds received β-glucans and MOS having higher average body weights values and significantly higher ND HI antibody titer than the other non medicated groups. Thymus, spleen and bursal indices of control negative showed significantly lower values than vaccinated medicated and non-medicated groups. Both total and differential leukocytic and lymphocytic counts showed significantly higher in medicated group than other groups. Liver function test showed lower AST and ALT in medicated group than other groups. Results of challenge test with NDV confirmed that MOS and B glucans immunostimulant improved protection rate by 15% in medicated than non- medicated ones. In conclusion MOS and B glucans can be given to chicken to improve both body weight and protection against VV NDV challenge that predominated in Egypt.
Immunomodulating,Effect,B-glucans,mannan,oligosaccharide,broiler,chicks,vaccinated,Newcastle,Disease,virus
https://jvmr.journals.ekb.eg/article_77626.html
https://jvmr.journals.ekb.eg/article_77626_ce58cf12b4efa81e2b79bde311e83457.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
20
1
2010
03
01
Effect of Di-ethyl aminoethyl (DEAE) Dextran on the infectivity titre of sheep pox virus in-vitro and in-vivo
303
305
EN
Olfat
E. Nakhla
Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt.
Namaa
A. Mohamed
Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt.
Manal
Awad
Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt.
Mervat
M. Ali
Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt.
10.21608/jvmr.2020.77629
The effect of diethyl aminoethyl (DEAE) dextran on the infectivity titre of sheep pox virus (SPV) was studied with different concentrations (25, 50, 75, 100 μg/ml) of DEAE-dextran on Vero cell culture. It was found that 25 and 50μg/ml were not toxic. The same concentrations were used with sheep pox virus inoculum showing that the best virus titre (106.3 TCID50/ml) reached with the use of 25μg/ml DEAE-dextran after 10 passages. The enhanced viral fluid was tested in-vivo, by vaccination of susceptible lambs and challenge of them with the virulent sheep pox virus. These lambs showed complete protection against the disease. The SP neutralizing antibody indices (NI) were estimated in the collected serum samples post vaccination and challenge; confirmed that 25μg of DEAE-dextran/ml virus-inoculum induced an increase in neutralizing antibodies in comparison with those induced by currently used sheep pox vaccine.
Effect,Di-ethyl,aminoethyl,DEAE,Dextran,infectivity,titre,sheep,pox,virus,Vitro,Vivo
https://jvmr.journals.ekb.eg/article_77629.html
https://jvmr.journals.ekb.eg/article_77629_623b6a5c2696faf17c9ecc87ceaed4d5.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
20
1
2010
03
01
Role of animal in occurrence of some zoonotic enteric protozoa in different areas of Nile Delta
306
312
EN
A. M.
Byomi
Department of Hygiene and Zoonoses, Faculty of Veterinary Medicine, Menoufia University-Sadat
City Branch, Egypt
H. A.
Samaha
General Organization for Veterinary Services, Egypt
S. A.
Zidan
Department of Hygiene and Zoonoses, Faculty of Veterinary Medicine, Menoufia University-Sadat
City Branch, Egypt
10.21608/jvmr.2020.77631
A total of 807 stool and fecal samples (251 stool samples from diarrheic children under six years old, 254, and 250 fecal samples from diarrheic and apparently healthy pre-weaned calves and lambs, respectively in addition to 50 fecal samples from dogs) were collected from different localities in Behera and Menoufia Governorates for detection of Cryptospridium spp., Giardia spp. and Entamoeba histolytica). Cryptosporidium spp. has been detected by using modified Ziel-Nelssen Stain (MZN) in 30 (11.95%); 26 (10.24%); 31(12.4%) and 2(3.84%) of the examined stool and fecal samples from children, calves, lambs and dogs, respectively in both Governorates. There were significant relationships between infection of the examined calves with Cryptospordium parvum and their age and healthy status. The same relation was noticed in concern with the examined children. Results of MZN were confirmed by using ELISA which was found sensitive (overall sensitivity 96.6%). In spite of the higher sensitivity of PCR than MZN for detection of C. parvum in fecal specimens especially when oocysts are scanty, the high cost of reagents and lack of expensive instruments which are not available in all clinical laboratories render MZN staining technique acceptable and reliable. By using direct smear and formal ether method, Giardia intestinals has been detected in {27(10.75%); 51(20.08%); 63(29.2%) and 5 (9.61%)} of stool and fecal samples from the examined children, calves, lambs and dogs, respectively from both Governorates. Calves, lambs and dogs seem to be important sources for Giardia intestinalis to man. Entamoeba histolytica has been detected in {19(7.56%); 0 (0),0(0) and 2(3.84%) of stool and fecal samples of the examined children, calves, lambs and dogs, respectively in both governorates. Dogs are regarded as an important source of Entamoeba histolytica to man.
role,Animal,occurrence,zoonotic,Enteric,protozoa,areas,Nile,DELTA
https://jvmr.journals.ekb.eg/article_77631.html
https://jvmr.journals.ekb.eg/article_77631_c6e8e814b9b2ebbb2cff64e1ce0df883.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
20
1
2010
03
01
Influence of water quality on fish productivity
313
318
EN
M. A.
El Bably
Department of Hygiene, Management and Zoonoses, Faculty of Veterinary Medicine, Beni-Suef
University
H. H.
Emeash
Department of Hygiene, Management and Zoonoses, Faculty of Veterinary Medicine, Beni-Suef
University
Asmaa
N. Mohamed
Department of Hygiene, Management and Zoonoses, Faculty of Veterinary Medicine, Beni-Suef
University
Nahla
R.
Department of Fish Diseases, Animal Health Research Institute, Dokki, Giza, Egypt
10.21608/jvmr.2020.77634
The physical, chemical and bacteriological parameters of water in fish ponds were investigated with a view to optimize the conditions for fish productivity by using three private fish farms with different water supplies. Water and fish samples were collected equally from each pond over a period of 17 months. Water temp., Dissolved oxygen (DO), pH, Ammonia, Nitrite, Nitrate, and bacterial count were determined. The average values of bacterial, parasitic, survival and growth rates of fish were also assessed. Results revealed that pond water in farm (III) had the highest averages of temperature nitrite, nitrate 31.8±1.5, 8.18± 0.9, 0.41 ± 0.06 , 3.79 ± 0.6 resp., with the least content of DO 3.6 ± 0.7 followed by farm (II),which had also the highest mean values of NH3 - N, total coliform and fecal coliforms were 3.15±0.65, 59.0 & 18.5 followed by ponds of farm (III), while, the lowest averages of estimated parameters were recorded in farm (I).Correspondingly, fish samples of farm (III) showed the highest level of total bacterial, coliform, fecal coliform count and parasitic infestation; were 42.5±5.4, 29.6±3.6 , 11.3±3.1 and 31.15% respectively, followed by fish of farm(II) 29.75±3.5, 11.5±3.3, 7.4±1.1 and 10.8 % respectively, Meanwhile, fish in farm (I) showed the lowest value of both bacterial contents with no parasitic infestation which explain the highest percentages of daily weight gain & survival rates 35.2±4.4 gm. & 97.0 ±1.6 % respectively. Throughout study period compared with those in farms (III & II) respectively, resulting from poor water quality & parasitic infestation which acts as stressors affecting fish health and productivity. Therefore, fish productivity can be enhanced if the water quality in the ponds were maintained at optimum levels.
Influence,water,Quality,fish,productivity
https://jvmr.journals.ekb.eg/article_77634.html
https://jvmr.journals.ekb.eg/article_77634_768e5fd57e6879447c5ad4378c587cee.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
20
1
2010
03
01
Effect of seasonal variations on performance and parasitic infestation of cultured fish in Fayoum governorate
319
323
EN
H. H.
Emeash
Department of Hygiene, Management and Zoonoses, Faculty of Veterinary Medicine, Beni-Suef
University, Beni-Suef, Egypt.
M. A.
El-Bably
Department of Hygiene, Management and Zoonoses, Faculty of Veterinary Medicine, Beni-Suef
University, Beni-Suef, Egypt.
Asmaa
N. Mohammed
Department of Hygiene, Management and Zoonoses, Faculty of Veterinary Medicine, Beni-Suef
University, Beni-Suef, Egypt.
10.21608/jvmr.2020.77635
A field study was carried out in a fish farm to study the effect of seasonal variations during the production period on performance and parasitic infestation of cultured fish. Water samples were obtained to determine the Physio-chemical investigation of water in the examined farm to estimate temperature, dissolved oxygen, PH, ammonia, nitrite, nitrate, salinity, total alkalinity and total hardness. Fish samples were obtained for parasitological examination to detect external parasitic infestation.The results referred that an elevation of water temperature during summer months leading to slight increase of PH of water, while dissolved oxygen values decreased from 6.8±0.15 to 6.0±1.5 throughout the study period. The mean values of ammonia, nitrite and nitrate reach the maximum in July and August months (0.71±0.18, 0.20±0.07 and 3.1±0.07 mg/l respectively). In addition there was a slightly increase of the total alkalinity and total hardness at beginning of the study (37.8±3.0 and 147.0±5.0mg/l respectively) reached the maximum levels in the summer months (44.4±2.8 and 182.8±6.0 mg/l respectively). It can be noticed that the most prevalent ectoparasitic affecting cultured Tilapia are Trichodina,Monogenia and Epistylis. It can be noticed that, the average initial body weight of examined fish was 6.8±2.3 gm and reached 218.0±3.8 gm. at the end of experiment (210 days) with an average of daily weight gain 1.01 ±0.07 gm. From the aforementioned results, it can be concluded that special attention to Physio-chemical parameters of water fish ponds and regular inspection of fish parasitic infestation particularly during summer months obtained a good fish performance as well as high body weight gain and high survival percentage.
Effect,seasonal,Variations,performance,Parasitic,Infestation,cultured,fish,Fayoum,governorate
https://jvmr.journals.ekb.eg/article_77635.html
https://jvmr.journals.ekb.eg/article_77635_ae8f8ac94037ff92d1958009d04428b0.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
20
1
2010
03
01
The prevalence of Infectious Bronchitis (IB) in some chicken farms in Egypt: I. Spotlight on the status of IB outbreaks in some chicken flocks
324
341
EN
K. M.
Kamel
Department of Poultry Diseases, National Research Center, Cairo
A. A.
Bassiouni
Department of Poultry
Diseases, Faculty of Veterinary Medicine, Cairo University.
M. A.
Afify
Department of Poultry
Diseases, Faculty of Veterinary Medicine, Cairo University.
N.S.
Rabie
Department of Poultry
Diseases, Faculty of Veterinary Medicine, Cairo University
10.21608/jvmr.2020.77637
Twenty five isolates of IBV were isolated from 36 broiler and layer chicken farms collected from 13 governorates during 2 years started from January 2003. Sixteen farms were vaccinated against IB, and 9 farms were not vaccinated. The cardinal signs of the disease in layers were drop in egg production, with watery albumen, inferior (pale and misshape shell) eggs, un-noticed respiratory distress and pectoral myopathy, and those in broilers were respiratory distress, renal urate deposition and death beyond 4 weeks of age (late mortality). The viruses were isolated and identified by chicken embryo, and CEK cell culture inoculation.
prevalence,infectious,bronchitis,IB,chicken,farms,Egypt,Spotlight,status,Outbreaks,flocks
https://jvmr.journals.ekb.eg/article_77637.html
https://jvmr.journals.ekb.eg/article_77637_cf381bf08a1bcb61cb5271219f30d332.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
20
1
2010
03
01
The prevalence of Infectious Bronchitis (IB) outbreaks in some chicken farms: II. Molecular characterization of field isolates of IB virus
342
350
EN
K. M.
Kamel
Department of Poultry Diseases, National Research Center, Cairo
A.
Khafagy
Department of Poultry
Diseases, Animal Health Research Institute, Dokki, Giza
A. A.
Bassiouni
Department of Poultry Diseases,
Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
M. A.
Afify
Department of Poultry Diseases,
Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
N. S.
Rabie
Department of Poultry Diseases,
Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
10.21608/jvmr.2020.77638
Twenty five isolates of IBV were isolated from 36 broiler and layer chicken farms collected from 13 governorates during 2 years started from January 2003. All the examined farms were vaccinated using the commercial live IB-H120 vaccine in addition to the IB-inactivated vaccine in the layer farm. The viruses were isolated and identified previously by chicken embryo, CEK cell culture inoculation. Isolates subjected to RT-PCR. Four isolates; three broiler farms and one from layer farm were genotyped using S1 partial gene sequencing. Typing of the four isolates using S1 partial gene sequencing, revealed that the isolated IBV strains showed homology to Asia, Europe, USA and Middle East strains.
prevalence,infectious,bronchitis,IB,Outbreaks,chicken,farms,Molecular,Characterization,field,isolates,virus
https://jvmr.journals.ekb.eg/article_77638.html
https://jvmr.journals.ekb.eg/article_77638_8beef7167a45efc441a2b40723b7bccb.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
20
1
2010
03
01
The prevalence of Infectious Bronchitis (IB) in some chicken farms in Egypt: III. Cross protection of vaccinated chickens versus field IB virus
351
363
EN
K. M.
Kamel
Department of Poultry Diseases, National Research Center, Cairo
A. A.
Bassiouni
Department of Poultry
Diseases, Faculty of Veterinary Medicine, Cairo University.
M. A.
Afify
Department of Poultry
Diseases, Faculty of Veterinary Medicine, Cairo University.
N.S.
Rabie
Department of Poultry
Diseases, Faculty of Veterinary Medicine, Cairo University.
10.21608/jvmr.2020.77639
Four groups of one-day-old SPF chicks were inoculated with the four IBV variants at 1 day old to study the virulence of these isolates. The results at 2 weeks post infection (PI) revealed that all isolates were able to induce serological resposne postinfection, respiratory distress and depression. 20% and 100% mortalities were recorded with isolates 4 and 23; respectively. Assessment of pathogenicity index and pathotyping (at the end of observation period “2wk-PI”), categorized the 4 tested isoaltes (4, 16,18, 23) into three isoaltes of high virulence (4, 18 and 23), and one isolate of intermediate virulence (16). About 50% reduction in body weight was recorded with the four IBV isolates 2 wk PI. Kidney lesions were nephritis-nephrosis with urate deposition in ureters, while microscopic lesions were associated with increase in the amount of rough endoplasmic reticulum (RER). Tracheal lesions recorded as increase the amount of mucin, while microscopic lesions were edema of mucosa and inflammatory cells in the lamina propria. The regime of administering the infectious bronchitis (IB) live commercial H120 vaccine at 1 day old SPF chicks, and the heterologous challenge with four variants (serotypes) at 4 weeks of age, was found to be poorly effective in protecting the respiratory tract of SPF chickens with protection percentages of 8.1%, 55%, 10.5% and 12.6% corresponding to field isolates of IBV 4, 16, 18 and 23; respectively. Protection was measured by assessing ciliary activity of the tracheal epithelium following challenge. It is suggested that the use of the live IB-H120 vaccine will not always broaden the protection against challenge with IB multiple serotypes isolated from Egypt. Therefore it is necessary to develop a new IB vaccines, either locally prepared or imported to overcome any new IB serotype that were emerged, through modifying vaccination strategies to make them appropriate to the field situation.
prevalence,infectious,bronchitis,IB,chicken,farms,Egypt,cross,protection,vaccinated,chickens,versus,field,virus
https://jvmr.journals.ekb.eg/article_77639.html
https://jvmr.journals.ekb.eg/article_77639_7e74e50cea2064114d0267184fbfbfe6.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
20
1
2010
03
01
Epizootiology of lumpy skin disease outbreak in cattle in middle of Egypt, 2006
364
373
EN
A. M.
El-Sherif
Department of Animal Medicine, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef,
Egypt
S. S.
Samir
Department of Pox, Veterinary Serum and Vaccine Research Institute, Abasia, Cairo,
Egypt
R. A.
Azam
Department of Animal Medicine, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef,
Egypt
Sherin
R. Roby
Department of Animal Medicine, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef,
Egypt
10.21608/jvmr.2020.77640
The current investigation studied an outbreak of lumpy skin disease of cattle in Beni-Suef and Al-Fayium governorates from March up to September 2006. Epidemiological data over a total of 5500 cattle from all ages, breeds and sexes were investigated. Prevalence of lumpy skin antibodies was screened by enzyme-linked immunosorbent assay (ELISA) that revealed high exposure rates; 57% and 51.42% in Beni-Suef and Al Fayium governorates respectively Virus isolation was conducted on chorioallantoic membrane (CAM) of specific pathogen free embryonated chicken egg (SPF-ECE) and MDBK cell culture. The virus identity was confirmed by passive haemagglutination and polymerase chain reaction (PCR) on the infected CAM and MDBK cell culture. Experimental infection of rabbits was successful, demonstrating their possible roles in the epidemiological process of the disease.
Epizootiology,lumpy,skin,Disease,outbreak,cattle,middle,Egypt,2006
https://jvmr.journals.ekb.eg/article_77640.html
https://jvmr.journals.ekb.eg/article_77640_3ef83dc1aa7bd3041cf9a6137b25b317.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
20
1
2010
03
01
Biomolecular relationship of whole protein of Mannheimia haemolytica, Pasteurella trehalosi and Pasteurella multocida of sheep
374
378
EN
Amal
M. El-Sawah
Central Laboratory for Evaluation of Veterinary Biologics, Abbasia, Cairo
Eman
M. El-Rawy
Veterinary Serum
and Vaccine Research Institute, Abbasia, Cairo
10.21608/jvmr.2020.77641
P. multocida contains one or more antigenic determinant of different proportions responsible for partial protection offered by the heterologous serovars. SDS-PAGE analysis of the whole protein profile prepared from sheep local isolates of P. multocida types (A, D), Mannheimia haemolytica type (A), P. trehalosi type (T) as well as standard strain of P. multocida (B6) revealed that the protein profile exhibited some differences with variable molecular masses ranged between 14 to 116 kDa. There are sharing protein subunits of molecular masses of 66, 37 and 28 kDa as the unique cross-reactive antigens in all isolates. The protection percentage for the vaccinated mice with inactivated M. haemolytica against the challenge with virulent strain of P. multocida type A, D and B6 are 30%, 30% and 20% respectively and 25%, 25% and 20% respectively in the groups of mice vaccinated with inactivated P. trehalosi so, there is cross reaction but limited cross protection between pasteurella isolates. The suggested vaccine must contain local isolates of P. multocida<br />serotypes A, D and B6 as well as M. haemolytica and P. trehalosi.
Biomolecular,relationship,protein,Mannheimia,haemolytica,trehalosi,Pasteurella,multocida,sheep
https://jvmr.journals.ekb.eg/article_77641.html
https://jvmr.journals.ekb.eg/article_77641_fe175ed1ad8425b43d3b07e53b4425bf.pdf
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
20
1
2010
03
01
Effect of Plocamium cartilagineum aegypticus on Boophilus annulatus
379
382
EN
Rania
A. Abd El-Wahab
Plant protection research institute, agriculture research center
10.21608/jvmr.2020.77642
Halogenated monoterpenes isolated from the red alga Plocamium cartilagineum aegypticus , proved their efficacy as acaricide. Both of Violacene and Mertensene, were adulticides with LC50's, 340.56 ppm and 759.23ppm, respectively, against the cattle tick; Boophilus annulatus adult females. Moreover, they can be used as ovicides. Violacene and Mertensene caused reduction in the hatchability by 96.77% and 95% respectively. Clinico-pathological studies were carried out and showed that Plocamium extract is safe to the host and none-target animals.
Effect,Plocamium,cartilagineum,aegypticus,Boophilus,annulatus
https://jvmr.journals.ekb.eg/article_77642.html
https://jvmr.journals.ekb.eg/article_77642_3f13268095fced2b4195a642a29d7ee9.pdf