ORIGINAL_ARTICLE
Pathogenicity of Escherichia coli O125 in commercial broiler chickens
Commercial broiler chickens are frequently infected with Ecoli serotypes in form of collibacillosis whichcharacterized as an initial respiratory infection (air sacculitis) followed by generalized septicemia, perihepatitis andpericarditis. The present work aimed to study the ability of E.coli O125 previuosly isolated and identified fromdiseased broilers suffered from pericarditis , airsacculitis and perihapatitis to induce a disease and The current studyaimed to determine the pathogenicity of E. coli serogroup O125 in broiler chicks, a trial of prevention and treatmentusing probiotic ( Guardyazin-m) or antibiotic (Cipronate20%). The chicks were divided into seven groups includingcontrol negative, orally infected , subcutenously infected , probiotic treated group, and finally antibiotic treatedgroup. The clinical signs, mortality, performance parameters and postmortem examination as well as thepathological changes in liver and intestine were recorded. The probiotic group gave the best results in controllinginfection by E. coli O125 followed by antibiotic. In conclusion, the E. coli O125 serogroup was pathogenic to chickswhen experimentally inoculated, so particular attention must be directed toward E. coli O125 as a pathogen infectingbird. Also probiotics was of great value in protection against the E. coli infection and improve the performanceparameters of chicks . Its effect on feed consumption , weekly body weight gain and feed conversion rate (FCR) wasdetermined.
https://jvmr.journals.ekb.eg/article_43328_af994e957e085626bed1b2f3841a966c.pdf
2019-06-01
1
8
10.21608/jvmr.2019.43328
chickens
Broilers
E. coli
Pericarditis
FCR
Asmaa
Abd Elatiff
1
Faculty of agriculture , El Minya University
AUTHOR
Azza
A. El-Sawah
2
Poultry Diseases, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt. 3
AUTHOR
Mohamed
M. Amer
3
Poultry Diseases , Faculty of Veterinary Medicine , Cairo University ,Giza12618, Egypt
AUTHOR
Al-Hussein
M. Dahshan
4
Poultry Diseases, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt.
AUTHOR
Hala
Salam
5
Bacteriology, Mycology and Immunology Disease, Faculty of Veterinary Medicine, Beni-Suef University
AUTHOR
Salama
A.S.Shany
6
Poultry Diseases, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt.
AUTHOR
ORIGINAL_ARTICLE
EFFECT OF SOME PLANT OILS ON REPRODUCTIVE ACTIVITIES IN FEMALE ALBINO RATS
h: 0px; "> The present study aimed to determine the effect of adding plant oils; extra virgin olive oil (EVOO), sunflower and soybean to animal feed on serum estradiol (E2) and progesterone (P4) levels, histological structure of ovaries and in vitro maturation of oocytes (IVM). A total of 60 mature female Albino rats were used. Animals were divided equally into 5 groups; control group (received standard diet), group II (received EVOO), group III (received sunflower oil), group IV (received soybean oil) and group VI (received oil mixture which consist of sunflower and soybean oils). After 6 weeks of feeding oil addited diet, blood samples were collected from all rats throughout the different stages of estrous cycle. Sera were used for determination of serum E2 and P4 levels. Only females that were not in estrus were scarified after the last blood sample collection, ovaries were harvested for histopathological examination and for in vitro maturation. Results showed that none of oils led to ovarian changes except soybean oil and oil mixture, cause congestion of some ovarian blood vessels. It wasalso noted that the hormonal pattern didn’t differ significantly among different treatments within the same stage of the cycle, except for the group received oil mixture where E2 and P4 levels decreased significantly (P < 0.05) during metestrus and diestrus phases, respectively. In the treated groups, the highest significant (P < 0.05) oocyte recovery rate (RR) (5.43 ± 0.23% and 4.41± 0.13%) and maturation rate (MR) (79.17 ± 2.03% and 73.43 ± 1.97%) were attained after application of EVOO followed by sunflower oil, respectively. While the lowest values were calculated with the soybean oil and oil mixture (3.83 ± 0.13 % and 2.50 ± 0.16 %) and (68.18 ± 2.29 % and 62.50 ± 2.23 %), respectively. It could be concluded that EVOO as well as sunflower oil have a beneficial influence on ovarian functional performance, retrieval of high number of good quality oocytes and raise oocyte maturation.
https://jvmr.journals.ekb.eg/article_43329_5e932f6bd9fa942c30e296e6c4fe6936.pdf
2019-06-01
9
21
10.21608/jvmr.2019.43329
EVOO
soybean oil
sunflower oil
in vitro maturation
fatty acids
Estrous cycle in rat
Estrogen and progesterone
Mabrouk
E.A.
1
Physiology Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 12452, Egypt.
AUTHOR
Ali
K.M.
2
Physiology Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 12452, Egypt.
AUTHOR
Nermeen
A. Helmy
3
Physiology Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 12452, Egypt.
AUTHOR
Rehab
M. Reda
4
Physiology Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 12452, Egypt.
AUTHOR
ORIGINAL_ARTICLE
Magnetic resonance imaging for detection of some foot lesions causing lameness in donkeys (Equus asinus)
"> Magnetic resonance imaging (MRI) is a good useful imagingtool for diagnosing foot lameness in equine. Seventeen donkeys of age (6–12 years), gender admitted to the clinic unit of our collage with owners complained that the animals were suffered from incurable lameness in their forelimbs. All forelimb foots were collected at the level of fetlock joint after euthanasia for performing MR imaging .The results showed that, both normal anatomy to the foot using T1-weighted image sequence and detection of different lesions involving many parts of the foot such as collateral ligaments of the DIP joint, both DDFT core and border, both navicular bone medullary cavity and cortex, articular cartilage of DIP joint, distal sesamoidean impair ligament (DSIL) and suspensory ligament of the navicular bone that could not be diagnosed by other diagnostic tools such as X- ray, ultrasound and Computed Tomography. In conclusion, Using MRI in examination of donkey foot lameness is considered as a definitive and accurate diagnostic tool for design suitable treatment regimens for all affections.
https://jvmr.journals.ekb.eg/article_43330_120c671d17cc72330fb8805ad96a2d79.pdf
2019-06-01
22
33
10.21608/jvmr.2019.43330
MRI Forelimb Foot
Affections Donkeys
Ragab
G. A.
1
Surgery, Anesthesiology and Radiology Department, Faculty of Veterinary Medicine, Beni-Suef Univ., Beni Suef 62511, Egypt.
AUTHOR
Fathy
M. Z.
2
Surgery, Anesthesiology and Radiology Department, Faculty of Veterinary Medicine, Beni-Suef Univ., Beni Suef 62511, Egypt.
AUTHOR
ORIGINAL_ARTICLE
EFFECT OF VIRGIN OLIVE OIL SUPPLEMENTATION ON LIPID PROFILE AND OXIDATIVE STATUS IN RATS
The aim of the present study is to investigate the effect of virgin olive oil on some blood parameters in male Albino rats supplemented with normal diet. thirty male Sprague Dawley rats, (90-110 g), were used in the present study, and were divided into three groups (10 in each), 1st group (control), received basal diet and supplemented with 1ml saline. 2nd and 3rd groups received basal diet, and supplemented daily with 1ml/100 gm B.W and 2ml/100 gm B.W of virgin olive oil (VOO), respectively for 4 weeks. Blood samples were collected weekly from all rats. Serum samples was obtained for assay of lipid profile levels and hepatic lipid peroxidation (MDA) enzyme. Blood lysate was used for antioxidant enzymes activities SOD, GPx and CAT.
https://jvmr.journals.ekb.eg/article_43331_51fe15d46fd0e259c81345ca16d71937.pdf
2019-06-01
34
40
10.21608/jvmr.2019.43331
Antioxidant parameters
kidney functions
Lipid profile
Virgin olive oil
Farahat
A. A.
1
Physiology Department, Faculty of Veterinary Medicine, Cairo University, , Egyp
AUTHOR
Sawiress
F. A.
2
Physiology Department, Faculty of Veterinary Medicine, Cairo University, , Egypt.
AUTHOR
Aghwider
A. A.
3
Physiology Department, Faculty of Veterinary Medicine, Cairo University, , Egypt.
AUTHOR
ORIGINAL_ARTICLE
EFFECT OF VIRGIN OLIVE OIL SUPPLEMENTATION ON SOME HEMATOLOGIC AND THYROID HORMONES, LEVELS IN RATS
The aim of the present study is to investigate the effect of virgin olive oil on some blood parameters in male Albino rats. Thirty male Sprague Dawley rats, (90-110 g), were used in the present study and were divided into three groups (10 in each), 1st group (control), received basal diet and supplemented with 1ml saline. 2nd and 3rd groups received basal diet, and supplemented daily with 1ml/100gm B.W and 2ml/100 gm B.W of virgin olive oil (VOO), respectively. Blood samples were collected weekly from all rats. Whole blood was obtained for determination of some haematological parameters, while sera were collected for the assay of T3 and T4 hormone.
https://jvmr.journals.ekb.eg/article_43332_70f80bc4194f76740200f5823e80de66.pdf
2019-06-01
41
47
10.21608/jvmr.2019.43332
Haematological parameters
T3 and T4 hormone
Virgin olive oil
progesterone
Farahat
A. A.
1
Physiology Department, Faculty of Veterinary Medicine, Cairo University
AUTHOR
Sawiress
F. A.
2
physiology Department, Faculty of Veterinary Medicine, Cairo University, Egypt.
AUTHOR
Aghwider
A. A.
3
physiology Department, Faculty of Veterinary Medicine, Cairo University, Egypt.
AUTHOR
ORIGINAL_ARTICLE
Serogrouping and resistance gene detection in avian pathogenic E.coli isolated from broiler chickens
E coli infection in poultry is one of the principle causes of mortality and morbidity in chickens and turkeys resulting in retardationof growth and decreased feed conversion rate . The most common form of collibacillosis is characterized as an initial respiratoryinfection (air sacculitis) followed by generalized septicemia, perihepatitis and pericarditis. The present study aimed to identify thebacteria associated with pericarditis , airsacculitis and perihapatitis in Egyptian broiler chickens. A total 300 samples of diseasedand dead broilers from 3-6 weeks age were collected. The isolated bacteria included E. coli, Klebsiella spp., Shigella spp. AndEnterobacter spp. Sero-grouping of the isolated E. coli strains revealed O125, O158, O55,O129, O20 , O6 , O8 , O27, O115,O142 and un-typed strains with prevalence of 16%, 12%, 8%, 8%, 8% , 8% , 8% , 8% ,4%,4% and 16% respectively. Themajority of E coli isolates were sensitive to colistin sulphate (38%) and Norofloxacin (38%) followed by ciprofloxacin (19%),cefotaxim (19%) and Ofloxacin (19%). On the other hand, E coli were resistant (100%)to amoxicillin, clindamycin ,Erythromycin and streptomycin. PCR analysis for antibiotic resistance genes of E coli detected that 12 serogroups isolates werepositive using the specific primers for Aada2 , BlaCTX and TetA(A) genes. The current study demonstrated the high prevalenceof E. coli indeed broilers suffered from pericarditis , perihepatitis and airsacculitis . Measures are needed to control E. colicontamination in poultry farms to reduce economic losses caused by infection of E.coli.
https://jvmr.journals.ekb.eg/article_43333_34d773af8a8d2c6a4513aa4ac5a461e0.pdf
2019-06-01
48
54
10.21608/jvmr.2019.43333
chickens
Broilers
E. coli
Pericarditis
FCR
Asmaa
Abd Elatiff
1
Veterinarian, Faculty of agriculture, El Minya University
AUTHOR
Azza
A. El-Sawah
2
Poultry Diseases, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt.
AUTHOR
Mohamed
M. Amer
3
Poultry Diseases, Faculty of Veterinary Medicine, Cairo University, Giza12618, Egypt
AUTHOR
Al-Hussein
M. Dahshan
4
Poultry Diseases, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt.
AUTHOR
Salama
A.S.Shany
5
Poultry Diseases, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt.
AUTHOR
ORIGINAL_ARTICLE
Antimicrobial and immunological studies on Pasteurella multocida and Mannheimia haemolytica recovered from calves affected with respiratory manifestations
Pneumonic pasteurellosis is the main cause of severe respiratory tract infections incalves and causing great economic losses. The objective of this research was to studythe antimicrobial susceptibility of P. multocida and M. haemolytica and detection theeffect of Lysozyme and Nitric oxide; as immune parameters, on most importantbacteria causing pneumonic pasteurellosis in cattle calves. A total number of 406 deep < br />nasal swabs and blood samples were collected from 406 bovine calves suffered fromrespiratory manifestations. Bacteriological examination revealed that the overallprevalence of both P. multocida and M. haemolytica was of 26.6%; 18.2% for P.multocida and 8.4%for M. haemolytica. P. multocida was singly isolated from 4.9% ofcases. While it was mixed with S. aureus, E. coli, Streptococcus spp., both S. aureus andE. coli, both S. aureus and Streptococcus spp. and both E. coli and Streptococcus spp.with percentages of 4%, 1.2%, 2.2%, 1.7%, 3.2% and 1.0%, respectively. Meanwhile,M. haemolytica was isolated as a single isolate from 1.7% of cases while it was mixedwith S. aureus, Streptococcus spp., both S. aureus and Streptococcus spp. and both E.coli and Streptococcus spp. with percentages of 2.7%, 1.2%, 2.5% and 0.2%,respectively. The in in-vitro sensitivity testing of all isolates showed high susceptibilityto Fluoroquinolones and cephalosporins. On the other hand, high resistances wereobtained against tetracyclines, penicillins and aminoglycosides. On the immunologicallevel, the data of the existing research show that all respiratory affected calves recordsignificant elevation of nitric oxide level in compare with normal control calves.However, all infected calves elucidate significant reduction of lysozyme activity.
https://jvmr.journals.ekb.eg/article_43335_e257dc8423b337c67f7b07a1c8cc75cb.pdf
2019-06-01
55
63
10.21608/jvmr.2019.43335
P. multocida
M. haemolytica
Antimicrobial
immune parameters Nitric oxide
lysozyme
El-Seedy
F.R.
1
Bacteriology, Mycology and Immunology Department, Faculty of Veterinary Medicine, Beni-Suef University, Egypt.
AUTHOR
Abed
A.H.
2
Bacteriology, Mycology and Immunology Department, Faculty of Veterinary Medicine, Beni-Suef University, Egypt.
AUTHOR
Hassan
H.M
3
Animal Reproduction Research Institute, Giza, Egypt.
AUTHOR
Nabih
A.M.
4
Animal Reproduction Research Institute, Giza, Egypt.
AUTHOR
Khalifa
E.
5
Microbiology Department, Faculty of Veterinary Medicine, Matrouh University, Egypt.
AUTHOR
Salem
S.E.
6
Animal Reproduction Research Institute, Giza, Egypt.
AUTHOR
ORIGINAL_ARTICLE
Correlation between Aspergillus fumigatus isolates recovered from human and broiler chickens
A total of 225 samples were collected from suspected cases and they were 75 human samples (40 ear swabs, 29 vaginal swabs and 6 sputum samples) and 150 from broiler chickens for microbiological examination and fungal isolation, they collected from different areas in El-Fayoum and BeniSuef governorates, from which 129 fungal isolates were recovered, 22 fungal isolates( 29.3%) were recovered from human; of which 15 isolates of ear swabs ( A.fumigatus 37.5%) while 7 (24.1%) A.fumigatus isolates were recovered from women, but there was no A.fumigatus recovered from sputum samples, as well as there were 53 A.fumigatus isolates (35.3%) recovered from broiler chicken. The antifungal activities of thymol and carvacrol oils against the recovered fungal isolates were tested using agar dilution method. Thymol and carvacrol oils completely inhibited the growth of different fungal isolates at concentrations of 1% and 0.1%. On the other hand, the concentration of 0.01% was too weak to inhibit the fungal growth, but it completely reduced the colour of the fungal colony converted it into white coloured arial mycelium. PCR assay using oligonucleotide primer that amplifying 250bp fragment in its Gene of A.fumigatus and A.niger was performed. Sequence analysis of two isolates of A.niger and A.fumigatus using its Gene was performed.
https://jvmr.journals.ekb.eg/article_43337_d38749c0a18ff098dfccbef154c2b104.pdf
2019-06-01
64
75
10.21608/jvmr.2019.43337
A.fumigatus
human
Broiler chickens
Radwan
I.A
1
Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Beni-Suef University, Egypt.
AUTHOR
Kamel
. M.F
2
Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Beni-Suef University, Egypt.
AUTHOR
Hamdy
.D.A
3
Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Beni-Suef University, Egypt. .
AUTHOR
Mahmoud
.Z.A
4
Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Beni-Suef University, Egypt.
AUTHOR
ORIGINAL_ARTICLE
Clinical evaluation of epidural bupivacaine, butorphanol, and butorphanol - bupivacaine combination in goat
0px; "> Butorphanol is a synthetic opioid commonly used for epidural anesthesia in humaneither alone or in combination with bupivacaine but not in goat. The objective of thepresent study was to evaluate the analgesic effect of epidural butorphanol eitheralone or in combination with bupivacaine. Fifteen adult apparently healthy goatswere randomized into three equal groups to receive a lumbosacral epidural injectionof bupivacaine 0.5 % (0.5 mg/kg), butorphanol 1% (0.08 mg/kg) or theircombination (bupivacaine 0.25 mg/kg and butorphanol 0.04 mg/kg). Animals wereobserved for incoordination of hind limbs, perineal pin prick and sedation at 10minutes interval. Heart rate, respiratory rate and rectal temperature were assessedevery 15 minutes. Epidural butorphanol resulted into significantly (P < 0.05) rapidanalgesia (9.8 ± 1.1 Min) than the butorphanol– bupivacaine combination (12.8 ±0.84 Min) and bupivacaine (16.6 ± 0.55). Butorphanol-bupivacaine combinationprovoked significant ((P < 0.05) prolonged intense analgesia (208 ± 8.36 Min)compared to either bupivacaine (112 ± 8.37) or butorphanol (166 ± 5.48 Min).Ataxia accompanied administration of bupivacaine either alone or in combinationwith butorphanol, while sedation was observed in animals received butorphanolalone or combined with bupivacaine. No significant changes were observed in heartrate, respiratory rate or rectal temperature. The epidural administration ofbutorphanol-bupivacaine combination promoted longer-lasting analgesia in goatswithout motor disturbances compared to bupivacaine alone. This combination mightprove useful clinically to provide analgesia in goats for long-duration perinealobstetrical or surgical procedures.
https://jvmr.journals.ekb.eg/article_43338_39f9d2263f6845465febfb42bf8429d2.pdf
2019-06-01
76
84
10.21608/jvmr.2019.43338
epidural
goats
Bupivacaine
butorphanol
Hagag
U.
1
Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt.
AUTHOR
Mahmoud
M.M.
2
Department of Animal Medicine (Veterinary Internal Medicine), Faculty of Veterinary Medicine, BeniSuef University, Beni-Suef 62511, Egypt.
AUTHOR
ORIGINAL_ARTICLE
Bacteriological studies on calves affected with respiratory manifestations
Bovine respiratory disease (BRD) is the most common and costly diseaseaffecting beef cattle calves in the world. The objectives of this study were to put ahigh light on epidemiology of P. multocida and M. haemolytica as important causesof bovine respiratory disease (BRD) in Egypt Governorates; Giza,El-Fayoum,BeniSuef, Assiut and Sohag. A total number of 406 deep nasal swabs and blood sampleswere collected from 406 bovine calves suffered from respiratory manifestationsreared in different Governorates in Egypt. Bacteriological examination wasachieved and isolation of P. multocida and M. haemolytica were attained. Theoverall prevalence of bothP. multocida and M. haemolytica was of 26.6%; 18.2%for P. multocida and 8.4%for M. haemolytica. EL-Fayoum Governorate showed thehighest prevalences while Beni-Suef Governorate showed the lowest prevalences.P. multocida was singly isolated from 4.9% of cases. While it was mixed with S.aureus, E. coli, Streptococcus spp., both S. aureus and E. coli, both S. aureus andStreptococcus spp. and both E. coli and Streptococcus spp. with percentages of4%, 1.2%, 2.2%, 1.7%, 3.2% and 1.0%, respectively. Meanwhile, M. haemolyticawas isolated as a single isolate from 1.7% of cases while it was mixed with S.aureus, Streptococcus spp., both S. aureus and Streptococcus spp. and both E. coliand Streptococcus spp. with percentages of 2.7%, 1.2%, 2.5% and 0.2%,respectively. In conclusion respiratory manifestations are very important disordersin bovine calves. P. multocida and M.haemolytica are the most common bacteriacausing infection.
https://jvmr.journals.ekb.eg/article_43339_721b665dc65564bdf0c78684b8024ebf.pdf
2019-06-01
85
90
10.21608/jvmr.2019.43339
P. multocida
M. haemolytica
Bovine respiratory disease
prevalence
El-Seedy
F.R.
1
Bacteriology, Mycology and Immunology Department, Faculty of Veterinary Medicine, Beni-Suef University, Egypt.
AUTHOR
Abed
A.H.
2
Bacteriology, Mycology and Immunology Department, Faculty of Veterinary Medicine, Beni-Suef University, Egypt.
AUTHOR
Hassan
H.M.
3
Animal Reproduction Research Institute, Giza, Egypt.
AUTHOR
Nabih
A.M.
4
Animal Reproduction Research Institute, Giza, Egypt.
AUTHOR
Khalifa
E.
5
Microbiology Department, Faculty of Veterinary Medicine, Matrouh University, Egypt
AUTHOR
Salem
S.E.
6
Animal Reproduction Research Institute, Giza, Egypt.
AUTHOR
ORIGINAL_ARTICLE
A study on the effect of age, breed and season on sexual behaviour, testosterone concentrations and scrotal circumference in Egyptian rams
h: 0px; "> The objective of this study was to detect the influence of age, breed and season on sexual behaviour, testosterone concentrations and scrotal circumference in Egyptian rams. The study was carried out at agricultural research center belonging to Minia University, Shosha village, Samalut city, El-Minia. Ten crossbred and five adult Ossimi rams were used during this study. Every ram was allowed to breed two ewes to evaluate its sexual behaviour for a twenty minutes test. Blood samples were collected three times per season at two weeks interval from each ram during the period of experiment to measure testosterone concentrations. Also, scrotal circumference was measured at the same frequency and time interval as blood samples. Sexual behaviour was better in old rams than young ones (p < 0.01), but it was not affected by breed or season (p>0.05). Serum testosterone level was higher at autumn (7.13±02.9 ng/ml) than at winter (5.54±0.29 ng/ml) in crossbred rams (p < 0.01), while in Ossimi rams, higher level was obtained in spring (8.24±.39 ng/ml) than in summer (3.99±0.40 ng/ml). Mean scrotal circumference was higher in older rams (30.20±1.20 cm) than younger rams (22.53±1.27 cm). There was no significant difference for measures of scrotal circumference between crossbred rams (30.86±1.09 cm) and Ossimi rams (30.93±1.06 cm). Season had no significant effect on scrotal circumference in crossbred rams, whereas in Ossimi rams the effect of season was significantly obvious with the highest and lowest values were recorded in spring (31.0±1.05 cm) and winter (30.60±1.17 cm) respectively. It could be concluded from this study that the age of rams had a great influence on their sexual behaviour and scrotal circumference, while the effect of breed and season on sexual behaviour and scrotal circumference was not clear.
https://jvmr.journals.ekb.eg/article_43340_b141e96dd6577d72154350d60d2ec9cb.pdf
2019-06-01
91
100
10.21608/jvmr.2019.43340
sheep
Sexual behaviour
Scrotal circumference and Testosterone
Ahmad
S. Mostafa
1
Department of Animal Behaviour, Management and Development of Animal Wealth, Faulty of Veterinary Medicine, Minia University
AUTHOR
Mostafa
M. Farghal
2
Department of Animal Behaviour, Management and Development of Animal Wealth, Faulty of Veterinary Medicine, Minia University.
AUTHOR
ORIGINAL_ARTICLE
Pesticides residues in retail meat and offal
Pesticides are used extensively especially in developing countries like Egypt to control pest either in animal or in agriculture, which may lead to harmful residues in foods of animal origin. The current study was conducted to estimate the residue level of OC and pyrethroid in 320 beef and sheep samples (160each) collected from different shops at Beni-Suef governorate during summer and winter season. The collected samples were liver, muscle, kidney, and fat (80 each; 4o from each animal species).Among fourteen organochlorine compound examined, only Alpha HCH was detected in samples of cattle and sheep collected through winter season in a level below the MRL, while through summer season, only Alpha HCH and Delta HCH were detected in sheep samples in a level below the MRL. Pyrethroid pesticides residues represented by cypermethrin, deltamethrin, Esfenvalerate, permethrin were not detected through winter season, while they were detected in muscles of cattle and fat of sheep through summer season, while Labdacyhalothrin, bifenthrin, cyfluthrin, Meothrin were detected in most of examined samples from different species through winter and summer seasons, most of these results revealed higher mean level than the maximum residue limits. From these results most of OC could not be detected may be due to these compounds not used science 1970, on other hand pyrethroid it still used nowadays in Egypt either in agriculture or as spray in animals to control ectoparasites spatially in summer season.
https://jvmr.journals.ekb.eg/article_43342_a8ea3b06951133e606d6abe88bc8ae44.pdf
2019-06-01
101
114
10.21608/jvmr.2019.43342
Pesticides residues
Gaschromatography
organochlorine
Pyrethroid
beef
sheep
Khalafalla
F.A
1
Food Hygiene Department, Faculty of Veterinary Medicine, Beni-Suef University.
AUTHOR
Abdel-Atty
N. S
2
Food Hygiene Department, Faculty of Veterinary Medicine, Beni-Suef University.
AUTHOR
Omima
I.Ali
3
Beni – Suef Provincial Lab. Animal Health Research institute Dokki, Giza.
AUTHOR
ORIGINAL_ARTICLE
Molecular characterization and genotyping of recent isolate of Infectious Bronchitis Virus (IBV) in Egypt
In this study a total number of 22 organ samples (including trachea, lung and kidney) from 22 broiler farms from northern Upper Egypt were collected from Mars 2017 to June 2018 from chickens showing clear clinical and pathological signs of Infectious Bronchitis. The samples were prepared and examined by real time RT-PCR for diagnosis of IBV. A total number of 11 samples were positive (50%) which were used for further isolation on SPF eggs by three blind serial passages. Positive samples that showed the pathogenic lesions of IB (curling and dwarfing of embryos) were collected and tested with real time RT-PCR (rRT-PCR) for more confirmation then a part from S1 gene sequence was amplified by RT-PCR and the product was sequenced and the data have been compared with other related IBV strains. The results indicate that the Egyptian virus in this study has an identity percent reached up to 89% with other recent Egyptian isolates. However, it reached 67% with classical vaccine strains like H120 and variant I like CR88 strain. The lowest identity was observed with M41 strain (59%) in this study. The phylogenetic tree compared to other isolates from Middle East and worldwide showed that this isolate is related to the IBV variant 2 group closely related to IBVEg/1265B/2012 strain and the Israeli strain IS/1494/06.
https://jvmr.journals.ekb.eg/article_43343_79d8c97d2357eb07133e4aac84e26621.pdf
2019-06-01
115
124
10.21608/jvmr.2019.43343
Molecular characterization
Genotyping
IBV
Egypt
Mostafa
M. Hammouda
1
Beni-Suef, Egypt, National Laboratory for Veterinary Quality Control on Poultry Production
AUTHOR
Abdelsattar
M. Arafa
2
National Laboratory for Veterinary Quality Control on Poultry Production; Animal Health Research Institute; Dokki, Giza, Egypt.
AUTHOR
ORIGINAL_ARTICLE
Factors affecting the immunogenicity of E. coli O78 vaccine in chickens
Bacterial disease still has serious problem in the intensive poultry production. In the recent years, particular concern has been raised by high incidence of poultry infections by E. coli. Analyses of antibacterial properties of essential oils have been carried out by range of researches. This experiment aimed to study the effect of immunomodulators on the immunogenicity of vaccine E. coli O78. In this study 250 broiler chickens were used. They were divided into 5 separated groups all groups vaccinated with E. coli O78 cebel coarse spray vaccine, except control group, 2 groups treated with immunomodulators, 3 groups challenged with untyped E. coli strain, all chickens housed in separated anavar. First group was control, 2nd group was vaccinated only, 3rd group was vaccinated and challenged, 4th group was vaccinated and received immunomodulators and 5th group was vaccinated, received immunomodulators and challenged. All chickens were observed daily food consumption, weight gained mortality rate, lesion, bioavailability, and weekly collected blood samples from 2-5 birds. The results were summarized as follows; immunomodulators have positive effect on B.W.G, decreased mortality and morbidity rate. The challenge enhanced the effect of E. coli O78 vaccine and there was marked improvement in bioavailability, B.W.G and immune defense against bacterial and respiratory diseases. Also, immunomodulators increased immunogenicity against bacterial disease through enhancing immune response system, and had synergistic effect with vaccination against E. coli.
https://jvmr.journals.ekb.eg/article_43345_8ca638ea8a8598a98377a1d423582cfc.pdf
2019-06-01
125
133
10.21608/jvmr.2019.43345
E. coli
Broiler chickens
immunomodulators
E. coli vaccine
INF-γ
IL-6
El-Seedy
F.R.
1
Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Beni-Suef University, Egypt.
AUTHOR
Aml
Mokhtar
2
Department Microbiology and Immunology, Faculty of Veterinary Medicine, Aswan University, Egypt.
AUTHOR
Eman
Khalifa
3
Department of Microbiology, Faculty of Veterinary Medicine, Matrouh University, Egypt.
AUTHOR
Sara
Sayed
4
Department Microbiology and Immunology, Faculty of Veterinary Medicine, Aswan University, Egypt.
AUTHOR
ORIGINAL_ARTICLE
Antimicrobial patterns of Avian Pathogenic Escherichia coli isolated from broiler chickens
E. coli infections in avian species are an economic threat to the poultry industry worldwide. The spread of MDR bacteria has been recognized as an increasing problem in the veterinary and medical fields. The current study aimed to investigate the phenotypic and genotypic antimicrobial patterns of avian Pathogenic E. coli isolated from broiler chickens. Results of in-vitro antimicrobial susceptibility testing showed that E. coli isolates were more sensitive to imipenem only (72.4%). On the other hand, they were highly resistant to most of used antimicrobials including ciprofloxacin (95.9%), amikacin (94.9%), cefotaxime sodium (92.9%), gentamicin (89.9%), cefotriaxone (89.9%), topramycin (87.8%), sulphamethoxazole/trimethoprime (85.7%), ceftazidim (84.7%). Also, they were resistant to cefoprazone (79.9%), doxycycline (72.4%) and amoxycillin/clavulinic acid (69.4%). All the tested isolates of E. coli (100%) were multi drug resistant (MDR). PCR was applied on 15 MDR E. coli isolates to determine 4 genes responsible for antibiotic resistance included ampC, blaCTX, blaSHV and tetA (A). The results revealed that that ampC and blaCTX genes were the most prevalent found in all isolates (100%) while tetA (A) and blaSHV genes were harbored in 14 isolates (93.3%).
https://jvmr.journals.ekb.eg/article_43346_44da7ed6eccafc1ea06c6d9ae2614c8e.pdf
2019-06-01
134
142
10.21608/jvmr.2019.43346
E. coli
Broiler chickens
resistance genes
antimicrobial sensitivity. PCR
Ismail
A. Radwan
1
Bacteriology, Mycology and Immunology Department, Faculty of Veterinary Medicine, Beni-Suef University, Egypt.
AUTHOR
Sabry
M. Tamam
2
Virology Department, Faculty of Veterinary Medicine, Beni-Suef University, Egypt.
AUTHOR
Abeer
A. El Sayed
3
Animal Health Research Institute Dokki, El Egypt
AUTHOR
Rania
O. Qurani
4
Virology Department, Faculty of Veterinary Medicine, Beni-Suef University, Egypt.
AUTHOR
ORIGINAL_ARTICLE
Phenotypic characterization of Escherichia coli isolated from broiler chickens
Colibacillosis is one of the most important diseases of chickens, resulting in significant losses. The current study aimed to investigate the prevalence of E. coli infections in broiler chickens detecting their phenotypic characters such as Congo red binding activity, serum resistance and proteolytic activities. Samples were collected from 297 broiler chickens of different ages from different farms in El-Fayoum Governorate during the period from April 2017 up to March 2018. Bacteriological examination showed that 98 E. coli isolates were recovered with a prevalence rate of 33%. Results of Congo red binding activity and serum resistance revealed that all E. coli isolates (100%) showed Congo red binding activity and survive for 1 and 6 hrs and grown for 18 hrs in the presence of serum. Results of proteolytic activity revealed that 43 E. coli isolates (43.9%) were able to digest casein of skimmed milk while 16 isolates (16.3%) were positive for gelatin liquefaction test.
https://jvmr.journals.ekb.eg/article_43347_ea1bad031010b81ffd24f9e71cf90e9b.pdf
2019-06-01
143
150
10.21608/jvmr.2019.43347
E. coli
Broiler chickens
Congo red binding
Serum resistance
Proteolytic activities
Ismail
A. Radwan
1
Bacteriology, Mycology and Immunology Department, Faculty of Veterinary Medicine, Beni-Suef University, Egypt.
AUTHOR
Sabry
M. Tamam
2
Virology Department, Faculty of Veterinary Medicine, Beni-Suef University, Egypt
AUTHOR
Abeer
A. El Sayed
3
Animal Health Research Institute Dokki, El Egypt
AUTHOR
Rania
O. Qurani
4
Bacteriology, Mycology and Immunology Department, Faculty of Veterinary Medicine, Beni-Suef University, Egypt.
AUTHOR