ORIGINAL_ARTICLE
Protein analysis for comparison between Salmonellae isolated from different poultry species
A total of 620 egg samples from different species (chickens, ducks and ostriches) and 1615 poultry samples (chickens, ducks, pigeons, quails, turkeys and ostriches) were examined for salmonella infection. 12 salmonella isolates were obtained from the egg samples (1.9%) and 67 isolates from poultry samples (4.1%). Salmonella isolates were serotyped into S. enteritidis (25 isolates), S. typhimurium (17 isolates), S. infantis (12 isolates), S. montivideo (7 isolates), 3 isolates for each of S. rubislaw and S. cerro , 2 isolates for each of S. virginia, S. agona, S. poona, and S. derby and 1 isolate for each of S. sandiago and S. kentucky. The incidence of isolation from different poultry species was discussed in details. Antibiogram of the isolated salmonellae against 10 different antibiotics revealed that norofloxacin, ciprofloxacin, cepheridin and gentamycin gave the highest activity against different salmonella isolates while amoxicillin, tetracycline, and nitrofurantoin showed the highest resistance rate. Pathogenicity of the isolated serovars was tested in chickens. All isolates were found pathogenic with various degree of virulence. SDSPAGE protein analysis for the salmonella isolated form different poultry species revealed 12 protein bands ranged from 22-289 kDa. The differences were insufficient for reliable differentiation between the isolates and accordingly, it could be used beside other molecular techniques in differentiation between the salmonella strains.
https://jvmr.journals.ekb.eg/article_77902_a58646a12e0bab5f0ababc314d687a3f.pdf
2007-03-01
1
9
10.21608/jvmr.2007.77902
protein
analysis
Comparison
Salmonellae
isolated
Poultry
Seham A.
El-Zeedy
1
Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo Egypt.
AUTHOR
Hussein K.
Eldeen
2
Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo Egypt.
AUTHOR
Jihan M.
Badr
3
Animal Health Research Institute, Dokki Giza, Egypt.
AUTHOR
ORIGINAL_ARTICLE
Trial to increase the sensitivity of Brucella antigens treated with Binary ethylene imine as inactivated agent
kills Brucella cells by causing lysis of the membrane, so the phenol-heat killed brucella antigen may lake specificity as a result of destruction the majority of proteins in the cell wall. Accordingly, attention was directed to produce antigen using binary ethylene imine as an inactivator. The produced antigen showed high specificity in detecting Brucella abortus and Brucella melitensis-infected animals, but sensitivity was not affected in comparison with the standard Rose Bengal antigen. In Enzyme immunotransfer blot (EITB), phenol–heat killed brucella cells showed only 3 bands (37.375, 23.47 and 7.83 kDa) that denotes denaturation for at least 6 bands whereas binary inactivated brucella cells showed similarity with non-treated ones
https://jvmr.journals.ekb.eg/article_77903_8b68acb99bfe9fb6f0a1a6cbfdfca389.pdf
2007-03-01
10
14
10.21608/jvmr.2007.77903
Increase
sensitivity
Brucella
antigens
Binary
ethylene imine
Agent
Hussein K.
Eldeen
1
Veterinary Serum and Vaccine Research Institute, Abbassia, Cairo, Egypt.
AUTHOR
Salwa S.
Awad
2
Veterinary Serum and Vaccine Research Institute, Abbassia, Cairo, Egypt.
AUTHOR
ORIGINAL_ARTICLE
Serodiagnostic studies on bovine leptospirosis in Beni-Suef Governorate
The present study was carried out in dairy farms experiencing low reproductive efficiency. Blood samples were collected from 84 cattle and 16 buffaloes suffered from infertility problems for detection and titration of leptospiral antibodies using the microscopic agglutination test (MAT). Eleven standardized leptospira serovars were used as living antigens for this purpose. Sixteen (19.05%) and 2 (12.5%) samples were found positive for L. interrogans serovar Icterohaemorrhagiae for cattle and buffaloes respectively, with titers ≥1:200. Antibodies against L. interrogans serovar Pomona were detected in 8 (9.52%) and 2 (12.5%) in cattle and buffaloes respectively with titers ≥1:400. Two cattle (2.38%) and two buffalo (12.5%) samples were positive for L. interrogans serovar Djasiman. On the other hand, two cattle samples were positive for both L. interrogans serovar Icterohaemorrhagiae and L. interrogans serovar Pomona. Second serum samples were rechecked for seroconversion from each positively reacted animal with 3-4 weeks interval.
https://jvmr.journals.ekb.eg/article_77904_9f409f7e2539442e9ceef35a232df9a0.pdf
2007-03-01
15
20
10.21608/jvmr.2007.77904
Serodiagnostic
studies
leptospirosis
bovine
Beni-Suef
W. H.
Hassan
1
Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt
AUTHOR
ORIGINAL_ARTICLE
Polymerase chain reaction for differentiation of Pasteurella multocida isolates from turkeys in comparison to strains incorporating in fowl cholera vaccine
In the present study, polymerase chain reaction (PCR) using random primer (E-20) was used to characterize and identify strains included in this study. Strains included 4 vaccinal reference strains of Pasteurella multocida, CU strain and 4 field isolates of Pasteurella multocida isolated from diseased turkeys which were identified biochemically and serologically as A:1, A:3, A3x4 and D:11. The obtained results revealed that all strains were reacted positively and in different manner with the E20 primer except the 2 field isolates. The results of these reactions demonstrated in terms of bands of different molecular weight specific to each strain. This can be used as a base for characterization and differentiation of strains involved in the present study as the 2 field strains A:1 and A:3 react with primer. Mouse protection test was performed by vaccination of mice with local fowl cholera oil adjuvant vaccine then challenge with virulent field strains A:1, A:3, D:12 and untypable isolates. Results revealed that the local fowl cholera adjuvant vaccine could protect mice against virulent challenge with A:1, A:3 and D:12 field strains but it could not be protect mice against untypable isolates
https://jvmr.journals.ekb.eg/article_77913_016073371edf24afa31b8888676b80e2.pdf
2007-03-01
21
24
10.21608/jvmr.2007.77913
Polymerase
chain
reaction
Pasteurella
multocida
isolates
turkeys
Strains
incorporating
vaccine
fowl cholera
Nahed I.M.M.
Khamis
1
Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt.
AUTHOR
Zeinab M.
Souror
2
Central Laboratory for evaluation of Veterinary Biologics, Abbasia, Cairo, Egypt.
AUTHOR
Hanan N.
Ibrahim
3
Central Laboratory for evaluation of Veterinary Biologics, Abbasia, Cairo, Egypt.
AUTHOR
S. M.
Aboul Saoud
4
Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt. 2
AUTHOR
ORIGINAL_ARTICLE
Preparation and evaluation of kits for detection of antibodies of Pasteurella multocida
Polyclonal hyperimmune serum against Pasteurella multocida type A:5, A:8 and A:9 was prepared in boskat rabbits. The indirect haemagglutination test (IHT) showed that such serum had an antibody titer of 1114. The immunoglobulins in the prepared antiserum were precipitated using saturated ammonium sulphate solution. Its concentration was adjusted to be 18mg/ml in normal saline then it was conjugated with horse radish peroxidase and evaluated through the application of double sandwich ELISA. It was successful to detect Pasteurella multocida antibodies in positive serum samples with strong positive reactions up to a dilution of 1:100 ofthe prepared conjugate.In the present study, polymerase chain reaction (PCR) using random primer (E-20) was used to characterize and identify strains included in this study. Strains included 4 vaccinal reference strains of Pasteurella multocida, CU strain and 4 field isolates of Pasteurella multocida isolated from diseased turkeys which were identified biochemically and serologically as A:1, A:3, A3x4 and D:11. The obtained results revealed that all strains were reacted positively and in different manner with the E20 primer except the 2 field isolates. The results of these reactions demonstrated in terms of bands of different molecular weight specific to each strain. This can be used as a base for characterization and differentiation of strains involved in the present study as the 2 field strains A:1 and A:3 react with primer. Mouse protection test was performed by vaccination of mice with local fowl cholera oil adjuvant vaccine then challenge with virulent field strains A:1, A:3, D:12 and untypable isolates. Results revealed that the local fowl cholera adjuvant vaccine could protect mice against virulent challenge with A:1, A:3 and D:12 field strains but it could not be protect mice against untypable isolates
https://jvmr.journals.ekb.eg/article_77908_7151038bf26ce761ee138e2dbf3d1a07.pdf
2007-03-01
25
27
10.21608/jvmr.2007.77908
Preparation
Evaluation
kits
antibodies
Pasteurella
multocida
Zeinab M.
Souror
1
Central Laboratory for Quality Control of Veterinary Biologics Abbassia, Cairo, Egypt
AUTHOR
A. A.
Badawi
2
Central Laboratory for Quality Control of Veterinary Biologics Abbassia, Cairo, Egypt
AUTHOR
Hanan M.
Ibarahim
3
Central Laboratory for Quality Control of Veterinary Biologics Abbassia, Cairo, Egypt
AUTHOR
ORIGINAL_ARTICLE
Bovine Parapoxvirus: Isolation and pathogenicity studies
A disease characterized by papules, nodules, vesicles, pustules and ulcers on teats and udder as well as drastic drop in milk production was seen among a cattle farm in Fayoum Governorate, Egypt. A virus was isolated by inoculation of vesicle and scrap homogenate pool from infected cattle into the chorioallantoic membrane of specific pathogen free embryonated chicken eggs. The virus was identified by presence of pock lesions, intracytoplasmic inclusion bodies on the chorioallantoic membrane, polymerase chain reaction and immunohistochemistry of the inoculated membrane. A novel pathogenicity model was developed via ear pinna inoculation of Swiss mice. The virus produced vesicular and ulcerative lesions at the site of inoculation in inoculated mice. The virus identity was confirmed by the presence of intracytoplasmic viral antigens by immunohistochemistry
https://jvmr.journals.ekb.eg/article_77909_ec1d3fb3bd192585d3214c84baf9a378.pdf
2007-03-01
28
34
10.21608/jvmr.2007.77909
bovine
Parapoxvirus
isolation
pathogenicity
studies
A. S.
Abdel-Moneim
1
Department of Virology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt
AUTHOR
S. M.
Tamam
2
Department of Virology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt
AUTHOR
ORIGINAL_ARTICLE
Clinical, haematological and some biochemical variations hypophosphataemia in buffaloes before and after treatment at Assiut Government
A total number of 28 buffaloes aged between 5-7years, body weight ranged between 400-500 kg, and belonged to private farms at Assiut Governorate constituted the materials of this study. Twenty of them showed the classical signs of hypophosphataemia while the other eight buffaloes were proved to be healthy by both clinical and laboratory investigations, used as control group. Biochemical analysis of blood sera showed a highly significant hypophosphataemia, hypocalcaemia and hypomagnesaemia in diseased buffaloes either pre or post treatment when compared with the healthy control ones. Meanwhile, the examination of blood showed marked decreases in erythrocytic count, haemoglobin concentration and packed cell volume in diseased animals. All parameters improved in affected buffaloes after 10 days of treatment. The chemical analysis of agronomical samples of soil and drinking water were done. The statistical analyses between the studied parameters were carried out in buffaloes before and after treatment.
https://jvmr.journals.ekb.eg/article_77910_b51d14b95b90f473bea9d880dc561496.pdf
2007-03-01
35
41
10.21608/jvmr.2007.77910
Clinical
haematological
biochemical
Variations
hypophosphataemia
buffaloes
Treatment
Assiut
M. E
Radwan
1
Animal Health Research Institute., Assiut Regional Laboratory.
AUTHOR
H. Z.
Rateb
2
Department of Animal Medicine, Faculty of Veterinary Medicine, Assiut University
AUTHOR
ORIGINAL_ARTICLE
Ultrasonography of normal, cystic and dysplastic kidney in cattle
Thirty four apparently healthy cattle (9 males and 25 females) of mixed breed (Balady X Friesen) were selected for ultrasonographic investigations in this study. Ultrasonographic measurements of vertical and horizontal diameters of kidney, the diameters of the renal parenchyma and the diameter of renal sinus were determined in the middle of right and left kidneys. Twenty nine cattle (9 males and 20 females), had normal ultrasonographic appearance of both right and left kidney while the other five cows had some pathological affections including cystic kidney and renal dysplasia in their left kidneys . The vertical diameter of the right kidney was (4.84±1.18 cm) , the horizontal diameter (9.16 ± 1.35 cm) , and the vertical diameter of the renal sinus was ( 3.54 ± 1.02 cm ) . The thickness of the renal cortex and medulla ( renal parenchyma) was (2.16 ± 0.46 cm ). On the other hand ,the vertical diameter of the left kidney was (5.89 ±1.13 cm), and the vertical diameter of the renal sinus was ( 3.83 ± 1.12 cm ). The thickness of the renal cortex and medulla (renal parenchyma) was (2.46 ± 0.35 cm ). It was concluded that the ultrasonographic values determined in this study can be used as references for the diagnosis of morphologic changes in the kidney of domestic dairy cattle
https://jvmr.journals.ekb.eg/article_77911_7d9553a7fe0243a24d4158e6def2358a.pdf
2007-03-01
42
49
10.21608/jvmr.2007.77911
Ultrasonography
normal
cystic
dysplastic
kidney
cattle
M. M.
Seif
1
Surgery, anesthesiology and radiology department, Faculty Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt
AUTHOR
H. A.
Bakr
2
Internal Medicine department, Faculty Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt
AUTHOR
ORIGINAL_ARTICLE
Gastric neobladder: an experimental study in dog
The urinary bladder of 15 clinically normal dogs was excised and the ureters wereimplanted into an isolated, vagotomized gastric segment derived from the fundic region of the stomach. The gastric segment was closed to form a neobladder. Continence was maintained with a "nipple valve" created at the tubularized end of isolated segment of stomach. Clinical, radiological, ultrasonographical, urine and blood analysis and histopathological examination were carried out for assessment of the technique. Eleven cases showed an apparently normal bladder function. Two cases suffered from renal hydronephrosis and other two suffered from incontinence. It was concluded that gastric neobladder urinary diversion is satisfactory for clinical use in dogs.
https://jvmr.journals.ekb.eg/article_77912_147b173a42467965240e95a7cb3a5cb3.pdf
2007-03-01
50
59
10.21608/jvmr.2007.77912
Gastric
neobladder
experimental
study
Dog
M. M
Seif
1
Department of Surgery, Anaethesiology and Radiology, Faculty of Veterinary Medicine Beni-Suef University
AUTHOR
M. S.
Aimen
2
Department of Surgery, Anaethesiology and Radiology, Faculty of Veterinary Medicine Beni-Suef University
AUTHOR
H. H.
Kamel
3
Department of Clinical Pathology, Faculty of Veterinary Medicine Beni-Suef University
AUTHOR