ORIGINAL_ARTICLE
Recent biological techniques for diagnosis of Chlamydophila abortus in aborted goats
Chlamydophila abortus (C. abortus) is one of the most important causative agents of enzootic abortion which has been caused a serious economic problem in domesticated and wild ruminants world wide. This study was aimed to diagnose C. abortus infection in aborted goats in Ras Suder Research Station (South Sinai) - Desert Research Center from 2004-2006. Twenty aborted cases from 130 pregnant nannies were recorded and examined serologically using complement fixation test (CFT). Eighty percent (16/20) of the aborted cases were serologically positive and 20% (4/20) randomly collected from apparently healthy pregnant nannies were also had antibodies against C. abortus. Pathological lesions were detected. Ten aborted fetal samples from serologically positive aborted nannies were subjected to diagnosis using Polymerase Chain Reaction (PCR) showed positive results at 119 bp. According to this result, PCR proved to be feasible, reliable, specific and sensitive diagnostic tool in diagnosis of C. abortus infection.
https://jvmr.journals.ekb.eg/article_77644_aa1b630d779be61b7a3aad0a49b35e2b.pdf
2010-03-01
1
6
10.21608/jvmr.2020.77644
biological
techniques
Diagnosis
Chlamydophila
aborted goats
Wafaa
A. Osman
1
Department o Animal Health, Desert Research Center, Cairo
AUTHOR
Azza
S. A. Goda
2
Department o Animal Health, Desert Research Center, Cairo
AUTHOR
Mona
A. Mahmoud
3
Department o Animal Health, Desert Research Center, Cairo
AUTHOR
T. R.
Abou EL Naga
4
Department o Animal Health, Desert Research Center, Cairo
AUTHOR
ORIGINAL_ARTICLE
Comparison between molecular and classical techniques for identification of Mycoplasma species isolated from mastitic ruminants
A total of 165 cows, 19 buffaloes, 192 sheep and 118 goats were examined for detection of Mycoplasma mastitis. The results revealed that 114 (69.59%) and 6 (31.57%) were clinically mastitic cows and buffaloes respectively while 51 (30.9%) and 13 (68.42%) were apparently healthy cows and buffaloes respectively. On examining the apparently healthy cows and buffaloes, 67 (32.84%) and 18 (34.61%) were subclinically mastitic cows and buffaloes respectively. Mycoplasmas were isolated in percentages of 8.9%, 5.5% from subclinically mastitic cows and buffaloes respectively and in percentages of 12.97%, 12.5% from clinically mastitic cows and buffaloes respectively. M. bovis was isolated from 8 (32%) and M. bovigenitalium from 7 (28%) and 10 (40%) unidentified Mycoplasma. Isolation of Mycoplasma from udder tissue in cows and buffaloes were in a percentage of 28.5% in cows while no Mycoplasma isolates were obtained from buffaloes' udder tissues. Application of PCR technique on these isolates and some of the negative samples was positive 100%. On the other hand, the results revealed that 82 of 192 (42.7%) and 43 of 118 (36.44) of the examined sheep and goats respectively were clinically mastitic. Isolation of Mycoplasma was from 11 (13.41%) and 17 (39.53%) of the examined sheep and goat respectively. Identification of these isolates revealed 8 (29%) M. agalactiae isolates and 20 (71%) unidentified Mycoplasma spp. Application of PCR technique on traditionally identified M. agalactiae isolates revealed negative results on using M. agalactiae specific primer while positive results were obtained for the same 8 isolates (100%) on using M. bovis specific primer.
https://jvmr.journals.ekb.eg/article_77645_64906e44b23dadc874f85f8b0fb920a6.pdf
2010-03-01
7
15
10.21608/jvmr.2020.77645
Comparison
Molecular
techniques
Mycoplasma
isolated
mastitic
ruminants
W. H.
Hassan
1
Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, BeniSuef University, Beni-Suef, Egypt
AUTHOR
Mona
A. El-Shabrawy
2
Department of Microbiology and Immunology, National Research Centre, Cairo, Egypt
AUTHOR
E. G.
Sadek
3
Department of Microbiology and Immunology, National Research Centre, Cairo, Egypt
AUTHOR
ORIGINAL_ARTICLE
An approach towards bacterial pathogens of zoonotic importance harbored by commensal rodents prevalent in Beni-Suef Governorate
This study was conducted in the period July 2009 through June 2010 to determine the role of commensal rodents in transmitting bacterial pathogens to man in Beni-Suef Governorate, Egypt. A total of 50 rats of various species were selected from both urban and rural areas at different localities. In the laboratory, rodent species were identified and bacteriological examination was performed. Seven types of samples were cultured from external and internal body parts of each rat. The identified rodent spp. included Rattus norvegicus (16%), Rattus rattus rattus (42%) and Rattus rattus frugivorus (42%). The results demonstrated that S. aureus, S. lentus, S. sciuri and S. xylosus were isolated from the examined rats at percentages of 8, 2, 6 and 6 %, respectively. Moreover, E. durans (2%), E. faecalis (12%), E. faecium (24%), E. gallinarum (4%), Aerococcus viridans (12%) and S. porcinus (2%) in addition to Lc. lactis lactis (4%), Leuconostoc sp. (2%) and Corynebacterium kutscheri (8%) were also harbored by the screened rodents. On the other hand, S. arizonae, E. coli, E. cloacae and E. sakazakii were isolated from the examined rats at percentages of 4, 8, 4 and 6 %, respectively. Besides, Proteus mirabilis (6%), Proteus vulgaris (2%), Providencia rettgeri (6%), P. aeruginosa (4%), Burkholderia cepacia (2%) and V. fluvialis (2%) were also recovered from the investigated rodents. It was concluded that considerable bacterial pathogens could be harbored in/on different body parts of the examined rodents and that commensal rodents prevalent in Beni-Suef Governorate supply multiple potentials through which they may act as sources of infection and occasionally represent a serious threat to the public health.
https://jvmr.journals.ekb.eg/article_77646_ccdaad784c33044e65a1f4c85a4c63d1.pdf
2010-03-01
16
24
10.21608/jvmr.2020.77646
Bacterial
pathogens
zoonotic
harbored
Rodents
Beni-Suef
W. H.
Hassan
1
Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt
AUTHOR
A. E.
Abdel-Ghany
2
Department of Hygiene, Management and Zoonoses, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt
AUTHOR
ORIGINAL_ARTICLE
Some pharmacological studies of cephradine in broilers
The pharmacokinetic aspects of cephradine were studied after intravenous (IV), intramuscular (IM), subcutaneous (SC) and oral administration of a single dose of 50 mg kg-1 b.wt. in chickens. Tissue distribution and residues of cephradine after repeated oral administration for 5 consecutive days were also estimated. After IV injection of cephradine in a dose of 50 mg kg-1 b.wt., the serum concentration time curves were best described by a two compartment-open model. The drug was rapidly distributed with a distribution half-life (t0.5(α)) of 0.120 h and apparent volume of distribution (Vdss) was 2.187 L kg-1. The drug was rapidly eliminated with a half-life of elimination (t0.5(β)) of 1.047 h and the body clearance (ClB) was 2.35 L kg-1 h-1. The drug was rapidly absorbed after IM, SC and oral administration as indicated by short half-lives of absorption (t0.5(ab)) of 0.154, 0.364 and 0.65 h., respectively. While the elimination half-lives (t0.5(el)) and systemic bioavailabilities were 0.859, 2.652, 1.74 h and 59.386, 84.5, 97.97 %, respectively. Repeated oral administration of cephradine (50 mg kg-1 b.wt twice daily) for 5 consecutive days caused no change in serum enzyme activities of ALT and AST but induced a significant increase in serum uric acid concentration at 72 to 120 hours post administration.
https://jvmr.journals.ekb.eg/article_77647_171f9f5253c7af9b9273fb59875d7442.pdf
2010-03-01
25
30
10.21608/jvmr.2020.77647
Pharmacological
studies
cephradine
Broilers
A.A.M.
El-Gendy
1
Pharmacology Department, Faculty of Veterinary Medicine, Beni-Suef University
AUTHOR
Abeer
M. Radi
2
Pharmacology Department, Faculty of Veterinary Medicine, Beni-Suef University
AUTHOR
M.A.
Tohamy
3
Pharmacology Department, Faculty of Veterinary Medicine, Beni-Suef University
AUTHOR
ORIGINAL_ARTICLE
Level of contamination with lead and aluminum in liver sandwiches served by street vendors and some restaurants with different hygienic practice
A total of fifty liver sandwiches served by street vendors and restaurants with different hygienic practices (25 of each), were collected from Giza Governorate. The levels of lead and aluminium residues were determined by using atomic absorption spectrophotometer. The mean values of lead residues in cooked liver sandwiches served by each of street vendors and restaurants were 1.68±0.51 and 0.73±0.2 mg/kg; respectively; as well as, it was constituted 6.2±2.1 and 4.3±1.1 mg/kg for aluminum residues, respectively. The levels of lead and aluminum residues in liver sandwiches served by street vendors were high as compared to that detected in restaurants. The sources of contamination with such elements (lead and aluminium) as well as the public health importance were discussed. The suggestive measures to avoid the contamination with such elements in cooked food were recommended.
https://jvmr.journals.ekb.eg/article_77648_17eb59ea936b70e556346baa0e6494ec.pdf
2010-03-01
31
34
10.21608/jvmr.2020.77648
Contamination
Lead
Aluminum
Liver
sandwiches
street vendors
restaurants
R. H.
Abdel-Dayem
1
Department of Food Hygiene Animal Health Research Institute Dokki, Giza
AUTHOR
N. M.
Marzouk
2
Department of Food Hygiene Animal Health Research Institute Dokki, Giza
AUTHOR
G.N.
Ali
3
Department of Food Hygiene Animal Health Research Institute Dokki, Giza
AUTHOR
ORIGINAL_ARTICLE
Detection of residues of some heavy metals in fish fillets in Egyptian markets
Fifteen samples of fish fillets were collected from different Egyptian markets to detect the residues of some heavy metals in them. The average weights of fish fillets samples were 250g. The samples were separately packed in polyethylene bag and sent to the laboratory for preparation and analysis. All equipment surfaces and utensils which used in this study were thoroughly cleaned with detergent, rinsed with water, dried and then rinsed with 2-propanol solution. The samples were stored at –2C prior to analysis. The mean levels of lead and cadmium in fish fillets collected from the Egyptian markets were 1.51 0.34 and 0.60 0.0.25 mg/kg; respectively. The concentration of lead in fish muscle were less than the permissible heavy metal limit in fish meat (2.0 mg kg) as stated by the World Health Organization, also these results were in line with the corresponding EU regulations. However the level of cadmium concentration was over than the heavy metal limits in fish meat (0.5 mg/kg) for the World Health Organization.
https://jvmr.journals.ekb.eg/article_77649_bf17a8066b8fa6eab6d34930adee51ed.pdf
2010-03-01
35
39
10.21608/jvmr.2020.77649
detection
residues
Heavy metals
fish
fillets
Egyptian markets
R. H.
Abdel-Dayem
1
Department of Food Hygiene, Animal Health Research Institute, Dokki, Giza
AUTHOR
H. M.
Soltan
2
Department of Food Hygiene, Animal Health Research Institute, Dokki, Giza
AUTHOR
N. M.
Marzouk
3
Department of Food Hygiene, Animal Health Research Institute, Dokki, Giza
AUTHOR
ORIGINAL_ARTICLE
The situation of poultry industry and related veterinary service in republic of Yemen, 2007
The purpose of the assignment is to provide technical assistance to support the preparation /strengthening of the currently drafted Integrated National Planning for disease prevention, require-ment from poultry products and required improvements for future. The obtained data here were col-lected from site visits and interviews with project owner as well as official documents and reports.
https://jvmr.journals.ekb.eg/article_77650_cf032323e123ee465e64834c21cda3c3.pdf
2010-03-01
40
45
10.21608/jvmr.2020.77650
Poultry
industry
veterinary
service
republic of Yemen
G. A.
Zohair
1
Department of animal production, Faculty of Agriculture, Sana'a University, Republic of Yemen.
AUTHOR