eng
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
2019-12-01
26
2
151
163
10.21608/jvmr.2019.66094
66094
Original Article
Microbiological evaluation of chicken meat products
F. A. Khalafalla
1
Fatma, H. M. Ali
2
A. El-Fouley
3
Department of Food Hygiene, Faculty of Vet. Med., Beni-Suef University, Egypt.
Department of Food Hygiene, Faculty of Vet. Med., Beni-Suef University, Egypt.
Department of Food Hygiene, Faculty of Vet. Med., Beni-Suef University, Egypt.
The aim of the present study was to compare the microbiological quality and safety of chicken products collected from a poultry processing plant and from the retail market. The collected samples represented 120 chicken product samples (mortadella, frankfurters, burgers, nuggets, fillet and fajita); 60 samples were collected from a poultry processing plant and 60 samples were from retail markets. For assessing the microbiological quality of these products, total bacterial count (TBC), most probable number (MPN) of coliforms and total mold and yeasts were determined. While, for evaluating the safety of collected products, Staphylococcus aureus, Salmonella, E. coli and Listeria monocytogenes were investigated. As well as, sensory evaluation of collected products was carried out. It was found that the bacterial counts in samples collected from processing plants were lower than corresponding samples collected from retail market. For instance, the obtained mean values of TBC in processing plant samples were 1x10, 4x102, 2x10, 2x10, 3x10 and 6x10 CFU/g in case of chicken mortadella, chicken frank, chicken nuggets, chicken burger, chicken fillet and chicken fajita, respectively. While for retail market samples, TBC mean values were 2x10, 2x10, 3x10, 3x10, 4x10 and 3x10 CFU/g in chicken mortadella, chicken frank, chicken nuggets, chicken burger, chicken fillet and chicken fajita, respectively. It was evident that most of examined chicken product samples either from processing plant or retail markets were contaminated with investigated foodborne pathogens, namely; Staphylococcus aureus, Salmonella, E. coli and Listeria monocytogenes, in addition to contamination with mold and yeasts. In conclusion, the rate of contamination of chicken products from retail markets was higher than corresponding products obtained from processing plant, which is attributed to contamination of chicken products through bad handling during transportation, storage and marketing, as well as growth of contaminants as a result of improper storage conditions including temperature and humidity.
https://jvmr.journals.ekb.eg/article_66094_bfa4776501a7c0f070fda9801e9ad20f.pdf
Chicken products
E. coli
Foodborne pathogen
Poultry meat safety
Salmonella
eng
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
2019-12-01
26
2
164
173
10.21608/jvmr.2019.66098
66098
Original Article
The occurrence and distribution pattern of Eimeria species among domestic pigeons in Minia, Egypt
Sahar M Gadelhaq
1
Asmaa HAbdelaty
2
Department of Parasitology, Faculty of Veterinary Medicine, Minia University, El-Minia, Egypt
Animal Health Research, Minia province Lab
Avian coccidiosis is an important parasitic disease affecting poultry and causes high economic losses in poultry industry, which acts as an important sector in the Egyptian national income. It is caused by genus Eimeria that belongs to subphylum apicomplexa. It affects domestic pigeons causing great losses, particularly in squabs. So, this study was conducted to investigate the prevalence of Eimeria species infecting domestic pigeons (Columbia livia) in Minia province. Intestines of 400 domestic pigeons collected from various poultry butcher shops as well as 103 pooled fecal samples of household pigeons were microscopically examined by the flotation technique. The recovered oocysts were morphologically identified. The overall prevalence of Eimeria species infection was 27.0% (108/400) and 72.82% (75/103) in the intestinal and pooled fecal samples respectively. Seasonally, the highest infection rate in the pooled fecal samples was in both spring and autumn (80.0%), while the lowest was in summer (56.0%). Meanwhile, the infection rate of the intestinal samples was the highest in winter (33.33%) and the lowest in autumn (20.0%). Moreover, The monthly infection rate of intestinal samples revealed that September and April had the highest rates;(55.0% and 41.62%). However, no infection rates were found in October and November, while monthly infection rate of fecal samples recorded that January and February showed 100% infection rate. Meanwhile, March and August, showed the lowest rate of infection 28.57% and 42.86% respectively. The morphological identification revealed the presence of four Eimeria species, Eimeria labbeana, E. columbarum E.columbae and E.labbeana-like, in pigeons in Minia province. Further studies are recommended to investigate the life cycle and molecular differentiation of Eimeria species infecting domestic birds.
https://jvmr.journals.ekb.eg/article_66098_43f9be6662211fee731b8c30f96d3f2c.pdf
Eimeria
Infection
Minia
Pigeons
prevalence
eng
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
2019-12-01
26
2
174
185
10.21608/jvmr.2019.66100
66100
Original Article
Effect of nisin on the viability of Staphylococcus aureus in kareish cheese
Arafa. M. S. Meshref
1
Gamal. M. Hassan
2
Emad. M. Riad
3
Walaa. A. Ashour
4
Food Hygiene Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt.
Food Hygiene Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt.
Bacteriology Department, Animal Health Research Institute, Egypt.
Animal Health Research Institute, Beni-Suef Branch, Beni-Suef 62511, Egypt.
Staphylococcus aureus is a common cause of food-borne disease worldwide and food poisoning. This study reports the effect of nisin (0, 10 and 12.5 ppm) against Staphylococcus aureus in manufactured kareish cheese. Nisin was effective in reducing S. aureus count in cheese; a reduction of S. aureus count was observed from the 2nd day of storage period. S. aureus in kareish cheese decreased gradually from 4x108 to (8×107, 6.5×107, 5.8×107 CFU/gm.) in the 1st week till reached at the end of storage period of the 4th week to (4 ×104, 1.1×103, 1×102 CFU/gm.) for cheese containing (0, 10 and 12.5 ppm) of nisin, respectively during manufacture and storage for a month in the refrigerator at 4 ˚C. The data obtained in this study suggested that the use of nisin-containing cheese can be an effective method of controlling the growth and multiplication of S. aureus in cheese.
https://jvmr.journals.ekb.eg/article_66100_c9ce9c15326a5b64dd67d3d16f0002f9.pdf
kareish cheese
Nisin
S. aureus
eng
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
2019-12-01
26
2
186
199
10.21608/jvmr.2019.66101
66101
A retrospective study on the relationship among different dry period lengths, udder health status and their possible effects on the reproductive performance of Holstein-Frisian cows
Abdeltawab A.Y. Khalil
1
Mahmoud M. Hussein
2
Theriogenology Department, Faculty of Veterinary Medicine, Beni-Suef University, Postal code 62511, Beni-Suef/Egypt
Theriogenology Department, Faculty of Veterinary Medicine, Beni-Suef University, Postal code 62511, Beni-Suef/Egypt
This study aimed to evaluate the effect of different dry period lnghts (DPLs) on the udder health and fertility during the subsequent lactation. The impact of subclinical mastitis (SCM) and clinical mastitis (CM) on the reproductive efficiency were also investigated. Holstein-Friesian cows (n=894) were included in the study of udder health, of which only multiparous cows (n=499) were included in the investigation of the impact of different DPLs on the udder health and reproductive efficacy in the subsequent lactation. Cows were classified according to the DPLs into 3 groups: Short (SDPL, 60 d). A limit of 200,000 somatic cell/mL milk was established to separate between healthy and mastitic udder status. Accordingly, samples with 500,000-1,000,000 cell/ml were referred to cows affected with SCM and samples with >1,000,000 cells/ml were mentioned to cows affected with CM. A higher proportion of infected udder was detected in cows with LDPL (39.19%) Moreover, a higher proportion of SCM was recorded for cows with LDPL (17.57%) compared with that recorded for cows with SDPL (8.0%) and TDPL (6.13%). A higher proportion of CM was recorded for cows with a long dry period (LDP, 8.11%) compared with that recorded for cows with SDPL (4.0%) or with TDPL (4.8%). Cows with SDPL showed the best intervals reproductive indices compared with that recorded for both TDPL and LDPL. Furthermore, LDPL was found to be associated with significantly lower CR (33.11±2.91%), lower PR (50.39±3.97%) and higher NSPC (1.98±0.37) compared with values that recorded for either SDPL or TDPL. Cows with either SCM or CM showed the longest interval reproductive indices, less CR and PR and number of inseminations to become pregnant compared with healthy udder cows. A lower proportion of affected udder was recorded for Primiparous cow (24.3%) compared with that showed by multiparous cows (32.46%). Cows affected with mastitis during the voluntary waiting period (VWP) showed longer intervals to the first detected estrus and first services while those affected during the service period (SP) showed longer calving to conception intervals, lower CR and lower PR especially when the SCC exceed 500,000 cell/ml milk. Cows experienced udder infection during the SP showed the lowest PR (32.54% & 36.44%) and required more NSPC. In conclusion, a significant linkage among different dry period lengths and both the incidence of mastitis and fertility were recorded, with the best results were recorded for cows with TDPL and SDPL. Extending the dry period above 60 days increased the incidence of both clinical and subclinical mastitis and exerted a negative impact on the reproductive performance. The occurrence of mammary infection during the VWP increased the calving to first estrus and first service intervals, while its occurrence during the SP prolonged the days open, and extremely reduced the conception and pregnancy rates.
https://jvmr.journals.ekb.eg/article_66101_dcbe2f6459ba1b1a5e8f5bc28f838863.pdf
Dairy cow
dry period
mastitis
Reproductive efficiency
Somatic cell count
eng
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
2019-12-01
26
2
200
213
10.21608/jvmr.2019.66106
66106
Original Article
Prevalence of proteases and other virulence genes in APEC associated with respiratory viral infections in broilers
Asmaa A. Tolba
1
Azza A. El-Sawah
2
Salama A. S. Shany
3
Poultry Diseases Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt.
Poultry Diseases Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt.
Poultry Diseases Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt.
Acute upper respiratory disease in chickens is a major cause of economic losses due to high mortality rates especially in poorly managed cases. Respiratory disease in poultry is initiated by variety of viruses, bacteria and fungi. The current study aims to investigate the prevalence of avian pathogenic E. coli (APEC), their proteases and other virulence genes in respiratory viral disease outbreaks in broiler chickens. Quantitative RT-PCR (qRT-PCR) was performed on samples from 25 farms with respiratory affections, APEC was isolated and virulence determinants in E. coli were investigated phenotypically and genotypically. E. coli was isolated from different flocks (100%, n=25). They were positive to Congo red binding (100%, n=25), iss gene (100%, n=25), iutA gene (92%, n= 23), tsh gene (24%, n=6), vat gene (20%, n=5). Presence of iss gene and CR binding proves that all isolates are APEC. Although the entire 25 APEC isolates carried more than one virulence gene; either 2 genes (n=17), 3 genes (n=7) and 4 genes (n=1), no effect of the number of genes harbored on the mortality rates in different flocks was observed. The presence of two serine proteases genes (tsh and vat) was confirmed in a total of 10 isolates (40% of the isolates) with positivity to tsh gene (24%) and vat gene (20%). qRT-PCR for detection of IBV-S1, AIV-H9, AIV-H5 and velogenic NDV-F genes revealed that 96% (n=24), 44% (n=11), 12% (n=3) and 4% (n=1) of 25 farms were positive to IBV, AIV-H9, velogenic NDV and AIV-H5, respectively. The results showed that among the 25 flocks, single viral infection was observed in 12 flocks (11 IBV and 1 AIV-H9), while mixed viral infections were detected in 13 flocks; IBV/AIV-H9 (n=9), IBV/velogenic NDV (n=3) and IBV/AIV-H9/AIV-H5 (n=1).The average mortality rate was the lowest in flocks infected with IBV, higher rates of mortality were observed in flocks infected with AIV-H9, velogenic NDV and AIV-H5. Flock age seems to affect the mortality rate in flocks infected with AIV-H9 where flocks aging 16:20, 21:25 and 26:30 days suffered from 2.38%, 8.13%, 11.48% average mortality rates, respectively.
https://jvmr.journals.ekb.eg/article_66106_c1fb91c62d2f8726ed00996f0411aeda.pdf
Broilers
chickens
E. coli
Proteases
Respiratory viruses
Virulence
eng
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
2019-12-01
26
2
214
234
10.21608/jvmr.2019.66110
66110
Original Article
Rescue effects of prenatal melatonin administration against bisphenol A- induced perturbations of reproductive and thyroid activities in male rat offsprings
Kamel M. A. Hassanin
1
Shawky S. Ibrahim
2
Ahmed Abdel-Wahab
ahmmedahmmed90@yahoo.com
3
Dina M. M. H. EL.Kossi
4
Abdel-Razik H. Abdel-Razik
5
1- Biochemistry Department, Faculty of Veterinary Medicine, Minia University, Minia 61519, Egypt
Physiology Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt
Physiology Department, Faculty of Veterinary Medicine, Minia University, Minia 61519, Egypt
Physiology Department, Faculty of Veterinary Medicine, Minia University, Minia 61519, Egypt
Histology Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt
The current study aimed to investigate the effects of prenatal melatonin “MLT” administration against bisphenol A “BPA”- induced infertility and thyroid dysfunction in male rat offsprings (First generation “F1”). For that purpose, fifty adult albino rats (40 females and 10 males) were used and classified equally into five groups (8 females and 2 males in each group). First group (control group) in which, pregnant rats were injected with 0.3 ml of vehicle /day. The second group (low dose BPA) where rats received a daily dose of 25 µg / kg B.W. The third group (high dose BPA) where rats received a daily dose of 250 µg / kg B.W. Fourth group (low dose BPA + MLT) where rats received a daily dose of 25 µg BPA /kg B.W. plus 10 mg MLT / Kg B.W. The fifth group (high dose BPA + MLT) where the rats received a daily dose of 250 µg BPA / kg B.W. plus 10 mg /Kg B.W. All rats within each group received their specific treatment daily with subcutaneous injection starting from the fourth day of pregnancy till full term. Then, the male offsprings of each group were selected and reared until the 60th day after birth. Serum and tissue samples were collected for analyses and microscopical examination. Although prenatal administration of both BPA doses didn’t affect the body weight gain and testicular weights of male offsprings, they reduced significantly the serum levels of testosterone and triodotyrosine when compared to the control group. Also, both BPA doses disturb significantly the oxidant/antioxidant ratio. Moreover, prenatal administration of both BPA doses affected negatively semen quality of the produced offsprings and induced marked histological alterations in their testes and prostate. Remarkably, all serological and histological alterations observed after BPA exposure were ameliorated significantly with MLT co-administration. Thus, prenatal MLT administration could be considered an optimal treatment to relieve many reproductive disorders, degenerative changes of testes and prostate and thyroid malfunction induced in male offsprings after gestational exposure of their dams to BPA.
https://jvmr.journals.ekb.eg/article_66110_8ebc4211d20ceba4a39c855e8d627996.pdf
bisphenol A
Male offspring
Melatonin
testis
testosterone
eng
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
2019-12-01
26
2
235
246
10.21608/jvmr.2019.67966
67966
Original Article
Taraxacum Officinale (Dandelion) Roots Extract Mitigates Doxorubicin-Induced HematoCardiotoxicity in Male Albino Rats
Dina R. S. Gad El-Karim
1
Department of Pathology and Clinical Pathology, Faculty of Veterinary Medicine, Alexandria University, Egypt.
The present study was designed to evaluate the probable ameliorative effect of dandelion extract against doxorubicin hemato-cardiotoxicity. To accomplish this study, four groups of male albino rats (n=7) were used as follow, Group I: served as a control group, Group II: received dandelion extract (200 mg/ kg), Group III: received doxorubicin (2.5 mg/kg) and Group IV: received dandelion extract and doxorubicin identically to groups II and III. Doxorubicin was administrated 3times/week for two consecutive weeks, while dandelion extract was administrated daily for two consecutive weeks before doxorubicin administration and continued during doxorubicin treatment. The results illuminated that, administration of doxorubicin has a deleterious effect on both of blood cellular components and cardiac tissues, which was indicated by significant pancytopenia (decrease in all blood cell types), elevated serum cardiac enzymes activity (CK-MB and LDH), increased serum level of cardiacrelated proteins (troponin I, atrial natriuretic peptide (ANP) and B-type natriuretic peptide (BNP) with a depletion of cardiac tissues antioxidant (GSH, and SOD enzyme) and elevated lipid peroxide (MDA) level in this tissues. Coadministration of dandelion extract with doxorubicin significantly alleviated its hemato-cardiotoxic effect which was reflected positively on hematobiochemical changes and cardiac histopathological alterations.
https://jvmr.journals.ekb.eg/article_67966_1a7c421aca90409a75a01dc96296281f.pdf
ANP
BNP
Dandelion
Doxorubicin
Hemato-cardiotoxicity
Rats
eng
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
2019-12-01
26
2
247
257
10.21608/jvmr.2019.67968
67968
The ameliorative effect of methanolic red carrot extract and vitamin E against cadmium-induced testicular toxicity in rats
Kamel M. A. Hassanin
1
Ahmed Abdel-Wahab
ahmmedahmmed90@yahoo.com
2
Ahmed A Mahmoud
3
Abdel-Razik H. AbdelRazik
4
Walaa I. E. Abdel-Badeea
5
Biochemistry Department, Faculty of Veterinary Medicine, Minia University, El-Minia, 61519 Egypt.
Phsiology Department, Faculty of Veterinary Medicine, Minia University, El-Minia, 61519 Egypt. 3
Chemistry Department, Faculty of Sciences, Minia University, El-Minia, 61519 Egypt.
Histology Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef University, 62512 Beni-Suef, Egypt.
Biochemistry Department, Faculty of Veterinary Medicine, Minia University, El-Minia, 61519 Egypt.
The current study aimed to investigate the effects of methanolic red carrot extract (MRCE) against cadmium intoxication on testis of adult Wister albino male rats. For that purpose, forty eight Wister albino male rats were randomly divided into four groups (12 rats per group). Group 1 (normal control), animals received corn oil. Group 2 (cadmium group), animals received cadmium chloride (CdCl2) at a dose of (5 mg/ kg BW). Group 3 (cadmium &Vit. E group), animals received vitamin E at a dose of (400mg/kg BW) and CdCl2at a dose of (5 mg/kg BW). Group 4 (cadmium & red carrot extract, animals received methanol: water red carrot extract (1:1) at a dose of 400 mg/kg and CdCl2 at a dose of (5 mg/kg BW). All rats were received their corresponding treatment orally by gastric gavage daily for 4 weeks. Result of organ weight analysis in Cd –exposed rats showed a decrease in testes weight. On the contrary, MRCE and vitamin E prophylactic co-treatments with cadmium showed significant increase in testis weights in comparison to cadmium group (P<0.05). Moreover, sperm concentrations were reduced markedly with cadmium while they were upturned greatly after prophylactic co-treatment with either MRCE or vitamin E (P<0.05). Moreover, cadmium induced a significant increase in testicular malondialdehyde (MDA) and significant decrease of total antioxidant capacity (TAC) but both MRCE and vitamin E supplementation succeeded markedly to produce a significant reduction in testicular MDA and noticeable increase of TAC level. Thus, MRCE and vitamin E could be considered optimal prophylactic treatments to protect the testis of rats from cadmium intoxication.
https://jvmr.journals.ekb.eg/article_67968_73b2a43fe051da4d8fef93a9a5f68d9e.pdf
cadmium
Methanolic red carrot extract
Oxidative Stress
Rats
testicular toxicity
Vitamin E
eng
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
2019-12-01
26
2
258
270
10.21608/jvmr.2019.67969
67969
Original Article
Cross-sectional Anatomy, Magnetic Resonance Imaging and Computed Tomography of Fetlock Joint in Camel
Ibrahim AH
1
Adam ZE
2
Mohamed Tawfiek
mgtawfiek@vet.bsu.edu.eg
3
Anatomy and Embryology Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt
Anatomy and Embryology Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt
Anatomy and Embryology Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt
The current study aimed to describe the normal cross-sectional anatomy, magnetic resonance imaging, and computed tomography of the fetlock joint in adult healthy dromedary camel. This study was carried out on twelve fetlock joints of the fresh cadavers from three camels. The gross examination of these camels revealed that they were normal with no orthopedic abnormalities. The cadaveric fetlock joints (n=12) were scanned using a computed tomographic (CT) scanner and a 1 Tesla MRI scanner. Then the joints were injected with a colored latex and sectioned into transverse, dorsal, and sagittal slices. Cross-anatomical sections were correlated with their corresponding CT and MR images for evaluation of the normal relevant anatomical structures that appeared with different signal intensities on the CT and MRI scans. The current investigation revealed that all the major soft tissue structures in the fetlock joint of the dromedary camel were clearly visualized on both CT and MR scans, except the short and the cruciate sesamoidean ligaments that could not be identified on both the CT and MR images. The anatomical sections with their corresponding CT and MR images obtained in this study could be used as a reference for subsequent clinical diagnosis and the interpretation of fetlock joint pathologies in dromedary camel.
https://jvmr.journals.ekb.eg/article_67969_5d5201ecf81029e0d79265d038c51d1f.pdf
anatomy
Computed tomography (CT)
camel
Fetlock joint
Magnetic Resonance Imaging
eng
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
2019-12-01
26
2
271
279
10.21608/jvmr.2019.67970
67970
Original Article
Normal Cross-sectional Anatomy and Magnetic Resonance Imaging of Pastern and Coffin Joints in Camel
Ibrahim AH
1
Adam ZE
2
Mohamed Tawfiek
mgtawfiek@vet.bsu.edu.eg
3
Anatomy and Embryology Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt
Anatomy and Embryology Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt
Anatomy and Embryology Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt
The present study aimed to describe the normal cross-sectional anatomy and magnetic resonance imaging of the pastern and coffin joints of the dromedary camel. This study was conducted on twelve distal limbs (fore and hind limbs) of fresh cadavers from three healthy adult camels. The specimens were normal with no orthopedic disorders. Twelve distal limbs were scanned using a 1 Tesla MRI scanner and then injected with colored latex to be sectioned into sagittal, dorsal, and transverse slices. Cross anatomical sections were matched with their corresponding MR images for identification and evaluation of the clinically relevant anatomical structures that appeared with different signal intensities on MRI scans. The present study revealed that all the soft tissues of the pastern and coffin joints of the dromedary camels were clearly depicted on the obtained MR images. However, the palmar/plantar ligaments of the pastern joint and ligaments of the navicular cartilage could not be identified on the MR images. The annotated anatomical sections with their corresponding MR images could be used as a normal anatomical reference for the interpretation of some clinical diseases in the pastern and coffin joints of the dromedary camel.
https://jvmr.journals.ekb.eg/article_67970_43e54094662300faff855d712d5950ef.pdf
anatomy
Coffin joint
camel
Magnetic Resonance Imaging
Pastern joint
eng
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
2019-12-01
26
2
280
292
10.21608/jvmr.2019.74372
74372
Original Article
Antimicrobial resistance and molecular characterization of pathogenic E. coli isolated from chickens
El Seedy, F.R
1
Abed, A.H
2
Wafaa, M.M. H
3
Bosila, A.S
4
Abeer Mwafy
5
Department of Bacteriology, Mycology and Immunity, Faculty of Veterinary Medicine, Beni-Suef University, Beni Suef 62511, Egypt.
Department of Bacteriology, Mycology and Immunity, Faculty of Veterinary Medicine, Beni-Suef University, Beni Suef 62511, Egypt.
Animal Health Research Institute, Dokki, Giza, Egypt.
Animal Health Research Institute, Beni Suef, Egypt.
Department of Microbiology, Faculty of Veterinary Medicine, NewValley University, Egypt.
Forty eight clinically diseased broiler chickens from different (n=18) farms from Beni Suef and El-Fayoum Governorates were subjected to euthanasia and post mortem examination. Lesions include airsaculitis, pericarditis and perihepatitis. Bacteriological examination showed that 22.9% of isolates were E. coli positive. Serogrouping of isolates revealed O125, O112, O91, O157, O115 and O25 % of each serogroups with an incidence rate of 18.2%, 9.1%, 9.1%, 9.1%, 9.1%, and 9.1%, respectively and four strains were untyped. Antimicrobial sensitivity tests against 17 antimicrobials showed that the most common resistance patterns were against penicillin, lincomycin, oxytetracycline, clindamycin, amoxycillin and erythromycin followed by nalidix acid and trimethoprim. On the other hand, the most potent antimicrobials were colistin sulphate, gentamycin, doxycycline and ceftriaxone followed by enrofloxacin, ciprofloxacin, norfloxacin, chloramphenicol and lastly ampicillin. PCR showed that all isolates had B-lactam resistant gene (blaTEM) and tetracycline resistance gene A (tetA) but only 18 % have quinolones resistance gene A (qnrA).
https://jvmr.journals.ekb.eg/article_74372_ff192155b292d4ee653d168d04c94b32.pdf
Broiler chickens
E. coli
multidrug resistance
O157