eng
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
2009-03-01
19
1
1
10
10.21608/jvmr.2009.77801
77801
Original Article
The impact of probiotic (Biovet®) on some clinical, hematological and biochemical parameters in buffalo-calves
H. A. Bakr
1
E. M. Said
2
M. M. Abd El-Tawab
3
M. S. Hassan
4
Department of Animal Medicine, Faculty of Veterinary Medicine, Beni-Suef University
Department of Animal Medicine, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt
Department of Animal Medicine, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt
Department of Animal Medicine, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt
This study was conducted on 25 male buffalo-calves, with age range, 6-7 months old (158 - 165 kg bwt), belonged to a private farm in Beni-Suef governorate. The animals were divided into three groups; control group (5 buffalo-calves) received probiotic-free ration, Group I and Group II (10 buffalo-calves in each). Buffalo-calves in groups I and II were orally administered with 15 and 25 g (Biovet®)/animal/day with respectively. The experiment lasted for 84 days. The effect of probiotic (Biovet®) supplementation on clinical, hematological and biochemical parameters as well as on the body weight gain in growing buffalo-calves were investigated. Hemoglobin concentrations, packed cell volume (PCV %), erythrocyte counts (RBCs) and total leucocytes counts (WBCs) of group I, and II revealed insignificant alterations comparing to control group. Insignificant variations of aspertate aminotransferase activities (AST), alanine aminotransferase activities (ALT), albumin, globulin, urea and creatinine levels of groups I and II were also recorded. The activities of alkaline phosphatase (ALP) in groups I and II buffalo-calves were significantly increased comparing to that in control animals. The levels of the total protein and the glucose levels in the probiotic-treated buffalo-calves increased significantly (P<0.05) comparing to that in control animals starting from 28th and 42th day till the end of the experiment respectively. The levels of triglycerides, cholesterol, HDL-cholesterol decreased insignificantly in (Biovet®) whereas LDL-cholesterol levels significantly decreased (P<0.05) in treated groups comparing to that in control animals. The T3 and T4 concentrations and body weight gain in probiotic-treated buffalo-calves significantly increased (P<0.05) in comparison to control group. The study declared that the probiotic (Biovet®) has obvious effect on body weight gain in buffalo-calves without any deleterious effect on animal health.
https://jvmr.journals.ekb.eg/article_77801_966193c6850da4754c7064fcc2263a6d.pdf
probiotic
Clinical
hematological
biochemical
Buffalo
calves
eng
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
2009-03-01
19
1
11
17
10.21608/jvmr.2009.77802
77802
Original Article
Some anatomical studies on the arterial supply and venous drainage of the tail of the buffalo "Bos Bubalis L."
M. R. A. Gad
1
Z. A. Adam
2
M. G. Tawfiek
3
Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt
Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt
Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt
Twenty tails of buffaloes were used in this study from apparently healthy adult animals. They are dissected to reveal their arteries and veins. Fourteen photographs illustrated the results in the study. The study revealed that the arterial supply of the tail comes from the median caudal, superficial caudal, deep caudal, dorsolateral caudal arteries and the tail is drained by five caudal veins; median caudal, two ventrolateral caudal and two lateral caudal veins.
https://jvmr.journals.ekb.eg/article_77802_9169d3cf355204883a21ae8816835982.pdf
Anatomical
studies
arterial
supply
venous
drainage
Buffalo
eng
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
2009-03-01
19
1
18
22
10.21608/jvmr.2009.77803
77803
Original Article
Some anatomical studies on the nerve supply of the tail of the buffalo ”Bos Bubalis L. ”
Z. A. Adam
1
M. R. A. Gad
2
M. G. Tawfiek
3
Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef62511, Egypt.
Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef62511, Egypt.
Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef62511, Egypt.
Ten tails of buffaloes were used in this study from apparently healthy adult animals. They were dissected to reveal their nerve supply. Eight photographs illustrated the results in the study. The study revealed that there are five pairs of caudal nerves, sympathetic and parasympathetic innervations of the tail are demonstrated.
https://jvmr.journals.ekb.eg/article_77803_114c593649ecf42a3bf3ea617092d7ea.pdf
Anatomical
studies
nerve
supply
tail
Buffalo
eng
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
2009-03-01
19
1
23
26
10.21608/jvmr.2009.77804
77804
Original Article
Detection of testosterone residues in farm fish tissue
H. R. Abdel-Dayem
1
Hanaa M. Soltan
2
G. Naser
3
Food Hygiene Department, Animal Health research Institute, Dokki, Giza
Food Hygiene Department, Animal Health research Institute, Dokki, Giza
Food Hygiene Department, Animal Health research Institute, Dokki, Giza
A total of one hundred samples of marketed fish farm; 25each of tilapia (Oreochromis niloticus), Mugil cephalus, Carp (Silver) and fry; were collected from different localities at Al- Behera and Kafr El-Shakh Provinces. The samples were examined for detection of testosterone hormone by Thin Layer chromatography (TLC) as well as it was quantitatively assayed by Radio-Immuno-Assay (RIA). The obtained result revealed that the testosterone residues were detected in flesh of farm fish of Tilapia (Oreochromis nilotocus) and Carp (Silver), each constituting 24% and 4 %; respectively but it could be failed to detect in each of Mugil cephalus and fry. The hormonal residues of testosterone was ranged from 3.25 to 34.9 ng/g with mean value 4.22±1.1 ng/g in positive samples of Tilapia, while only one sample of Carp showed 22.0 ng/g, Mugil cephalus and Fry showed no detectable level of hormonal residues. The public health significance of detectable levels was discussed as well as the recommendations to avoid health hazards from such fish were mentioned.
https://jvmr.journals.ekb.eg/article_77804_d41d8cd98f00b204e9800998ecf8427e.pdf
detection
testosterone
residues
Tissue
fish
eng
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
2009-03-01
19
1
27
33
10.21608/jvmr.2009.77805
77805
Original Article
Field study on control of chronic respiratory disease in vertically infected broiler chicks
M. M. Amer
1
K. M. El-Bayomi
2
Zenab, M. S. Gera
3
A. E. A. Hanafei
4
Poultry Diseases Department, Faculty of Vet. Med., Cairo University.
Poultry Diseases Department, National Research Center, Dokki. 3
Poultry Diseases Department, National Research Center, Dokki.
El-Watania Poultry Co. Giza, Egypt.
Our field studies had been carried out after in vitro antibiogram of E. coli to compare the effect of pulmotil (macroloide), enerofloxacin (fluoroquinolones) and doxycycline (tetracycline) in controlling mycoplasma and E. coli as a cause of CRD in broilers. The drugs were used in single or in combination. Two doses at the 3rd and 23rd day of age on performance of commercial broiler Ross derived from mycoplasma SPA-test positive breeders and E. coli positive isolation at the 1st day of age. The prevalence of marked air sac gross lesions in non treated control group indicated the development of CRD and severity of lesions increased with age. The used drugs played a role in controlling infection as treated groups showed milder lesions while more sever lesions were in doxycycline treated group. Protection against mortality was less in the treated pins than untreated ones. Cumulative culls % was low (1.1) in pen treated with enrofloxacin, (1.5) in pulmotil + enrofloxacin, (1.6) in doxycycline, and (1.7) in pulmotil + enrofloxacin; while pulmotil and control were the same (2.2%). Losses expressed as total mortality and culls % were the lowest in pulmotil + enrofloxacin and enrofloxacin (3.2 and 3.6), other treated pins showed the same values (4.2), while the highest was in non treated ones (5.8%). Average Body wt. in pulmotil + enrofloxacin, pulmotil, and enrofloxacin treated pens were higher (1934, 1924 and 1819 gm) than doxycycline (1802 gm), Pulmotil + Doxycycline (1705 gm) and non treated control (1708 gm). CFCR in pulmotil or enrofloxacin and in combination medicated pens were higher than other treatments and non medicated pen. Average day/ week/ gain in control non treated was equal to that of pulmotil or enrofloxacin (65g), slight lower value was in their combination (63g) followed by 58 g in doxycycline. The lowest ADG /w/g value was in pulmotil + doxycycline (52 g). Calculated EEF of treated and non medicated pens were higher than > 280. The medicated pens with either pulmotil or/ enrofloxacin and there compilation were superior (333, 313 and 330; respectively) and close to the farm stander (346). This study pointed out that E. coli, and Mycoplasma with life ND vaccine reduced broiler performance and the used drugs were of values in control such infections. The in vitro antibiotics sensitivity testing of E. coli is important to obtain good results and drug combinations must be carefully performed.
https://jvmr.journals.ekb.eg/article_77805_2e49c4f27f2cda5b2916782cca3f2db8.pdf
Field study
control
chronic
respiratory
Disease
broiler chicks
eng
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
2009-03-01
19
1
34
38
10.21608/jvmr.2009.77806
77806
Original Article
Studies on the cumulative effect of sodium thiomersal on broilers vaccinated with inactivated poultry vaccines
Zeinab M. Sror
1
Anhar Abd El-Moety
2
Hanan, M. Ibrahim
3
M. L. Sayed, A. R. Mahmoud
4
A. R. Mahmoud
5
S. M. Shafei
6
M. H. Khodeir
7
Central Laboratory for Quality Control of Veterinary Biologics
Central Laboratory for Quality Control of Veterinary Biologics
Central Laboratory for Quality Control of Veterinary Biologics
Animal Health Research Institute
Animal Health Research Institute
Central Laboratory for Quality Control of Veterinary Biologics
Veterinary Serum and Vaccine Research Institute, Abassia, Cairo, Egypt
Merthiolate (Thiomersal) is known to be used as antimicrobial agent in inactivated vaccines without affecting vaccine potency. The present work investigated the effect of thiomersal contents in ND, AI and IBD inactivated vaccines on liver and kidney functions of vaccinated birds. The histopathological effect and the withdrawal time of such mercurial product from vaccinated chicken muscles were also investigated. Results revealed that residual thiomersal contents in ND, AI and IBD were 0.03; 0.02 and 0.03mg/ml respectively. Liver and kidney function parameters showed significant increases in serum activities of alanine aminotransferase (ALT) and serum aspartate aminotransferase (AST) up to the 4th week post vaccination. Serum urea was significantly decreased on the 3rd week in vaccinated birds compared to control ones. Significant increase in serum creatinine in vaccinated chickens was recorded on the 5th week post vaccination. Liver and kidney functions’ parameters remain high allover the experimental period (8 weeks). The histopathological examination of liver specimens revealed degeneration of hepatic cells and congestion of the central vein with inflammatory cell infiltration and congestion of blood vessels as well as coagulative necrosis. The spleen of vaccinated chickens showed depletion of lymphocytes while lungs showed thickening of the alveolar. Mercury contents in muscle were 0.72, 0.52; 0.046 and 0.00 mg/kg on the 1st, 2nd, 3rd and 4th week post last vaccination that considered safe to the consumer. It is recommended that vaccinated poultry with inactivated vaccines containing thiomersal should not be slaughtered before at least 4 weeks and it is preferable to use combined inactivated vaccines to reduce the thiomersal contents as possible.
https://jvmr.journals.ekb.eg/article_77806_82b9fab9489334ca81822dbf32a6ad10.pdf
studies
cumulative
Effect
sodium
thiomersal
Broilers
vaccinated
Poultry
vaccines
eng
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
2009-03-01
19
1
39
43
10.21608/jvmr.2009.77807
77807
Original Article
Effect of dietary selenium and vitamin E supplementation on productive and reproductive performance in rams
A. A. Baiomy
1
A. E. A. Mohamed
2
A. A. Mottelib
3
Animal Production Department Faculty of Agriculture, South Valley University, Qena, Egypt.
Animal Medicine Department Faculty of Veterinary Medicine, South Valley University, Qena, Egypt.
Animal Medicine Department Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt.
The studies were carried out on 32 rams of the ossimi breed (270 days of age). Sixteen rams (group 1) received a ration containing 0.2 ppm. Se , and 30 mg vit.E, the other 16 rams (group 2) received 0.5 ppm. Se, and 50 mg vit.E/kg feed mixture .The feeding test was conducted from 270 days until 360 days of age. During the experiment the rams were subjected to live evaluations, i.e. testes circumference, libido level, semen characteristics, as well as selenium concentration and glutathione peroxidase (GSH-Px) activity in blood serum and seminal plasma. Routine macroscopic and microscopic analyses of semen quality were accompanied by measurement of Se content and GSH-Px activity in blood and semen. The Se concentration in blood plasma, seminal fluid and spermatozoa was measured by fluorometric method, while the GSH-Px activity by method based on NADPH - coupled reaction .Comparing the results in animals of both groups , it was clear that the all were in good health conditions as ascertained by clinical examination. No significant variation was detected in body weight of animals of both groups. Significant elevation (p < 0.05) in the values of semen conc., semen conc./ ejac , semen motion , sperm motility / ejac.as well as total sperms count were observed in animals of group 2 as compared to those of group 1 .
https://jvmr.journals.ekb.eg/article_77807_847cfcdcdbec765f36838dec58b7fb99.pdf
Effect
dietary
selenium
vitamin
productive
reproductive
performance
rams
eng
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
2009-03-01
19
1
44
50
10.21608/jvmr.2009.77808
77808
Original Article
Studies on Edwardsiella tarda infection in catfish and Tilapia nilotica
Heidy Abo El-Yazeed
1
Mai D. Ibrahem
2
Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Giza Egypt
Department of Fish Diseases and Management, Faculty of Veterinary Medicine, Cairo University, Giza Egypt
Edwardsiella tarda was recovered from cultured freshwater catfish (Claries gariepinus) with a prevalence rate of 0.42% in summer season. Experimental infectivity studies of E. tarda in Catfish and Tilapia nilotica were done using the intraperitoneal (I/P) and intramuscular (I/M) routes. Experimentally infected Catfish with a dose of 0.3 ml from E. tarda bacterial suspension of 108 CFU/ml manifested pathognomonic clinical signs and postmortem lesions than the experimentally infected Tilapia nilotica. Application of PCR technique for rapid detection of E. tarda infection in the experimentally infected fishes gave positive reaction in 4th day post infection in catfish and the 7th day post infection in Tilapia nilotica.
https://jvmr.journals.ekb.eg/article_77808_0963a4bea1735e1fb817f6c2d293c1a7.pdf
Studies . Edwardsiella
Infection
catfish
Tilapia nilotica
eng
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
2009-03-01
19
1
51
56
10.21608/jvmr.2009.77809
77809
Original Article
Mineral status in blood serum of newborn calves in Assiut Governorate
M. A. Mohammad
1
Biochemistry unit, Animal Health Research Institute, Assiut Lab., Assuit, Egypt.
The dynamics of some serum mineral concentrations during the first weeks of life of native and crossbred newborn calves in Assiut governorate were investigated. Blood samples of 25 Balady and 25 crossbred (Friesian x native) male calves were investigated. Blood was drawn from calves at 1, 7, 14 and 21 days after parturition. Serum levels of calcium (Ca), phosphorous (P), magnesium (Mg), sodium (Na), chloride (Cl), potassium (K), iron (Fe), Copper (Cu) and zinc (Zn) were monitored. Levels of Ca, P, Fe, Cu and Zn increased (P < 0.05) in relation to age of Balady and crossbred calves when compared by the day 1 of age. In contrast, decreased in concentrations of Na and Cl (P < 0.05) in relation to age of Balady and crossbred calves when compared by the day 1 of age were detected whereas Mg and K values remained unchanged. Native calves had higher (P < 0.05) Ca, P and Fe in the 7th day than crossbred calves whereas Na and Cl were higher (P < 0.05) in native calves than crossbred calves at the first day after birth. Results from this study suggested that breed and age may play an important role in mineral homeostasis during the first weeks of life in the newly borne bovine calves.
https://jvmr.journals.ekb.eg/article_77809_f47d2cda6f9d581fd972dc9221ce8d72.pdf
Mineral
status
Blood
serum
Newborn
calves
Assiut
eng
Beni-Suef University; Faculty of Veterinary Medicine
Journal of Veterinary Medical Research
2357-0512
2357-0520
2009-03-01
19
1
7
12
10.21608/jvmr.2009.77811
77811
Original Article
Clinicopathological studies on the antioxidant effect of barley on chicken affected by lead toxicities.
Samia M. Mohamed
1
Animal Health Research Laboratory, Beni Suef 62511, Egypt.
Forty five, 21-day-old balady chick were used in this experiment. Chicks were divided into three equal groups. The 1st group used as control. The 2nd and 3rd groups were supplemented with lead (Pb) acetate (1500 ppm) in drinking water but the latter group received ration containing 20% barley. Blood samples were collected from the wing vein after 1, 2 and 3 weeks. RBCs count Hb concentration and PCV were significantly decreased in group II during the experimental period. RBCs indices showed a significant decrease in MCH and MCHC in group II after 2 and 3 week of experiment. Leukogram showed heteropenia and moncytopenia. Total protein values showed significant decrease in group II due to decrease in serum albumin level after 2 and 3 week of experiment. Significant increase in activity of liver enzymes AST & ALT and serum uric acid were observed in group II after 3 weeks of experiment. Measurement of serum level of malondialhyde (MDA) revealed a significant increase in group II after 2 weeks of experiment while the total antioxidant capacity (TAO) showed significant decrease in group II after 2 and 3 weeks of experiment. Results of the lead residues in the experimental groups revealed that lead residue in muscle, liver and kidney were rise in group II compared with groups I and III.
https://jvmr.journals.ekb.eg/article_77811_94ac572ccb6f2d4cc93f5c809552e0b6.pdf
Clinicopathological
studies
antioxidant
Effect
Barley
chicken
lead toxicities